Adenovirus Ao Whānui
Ingoa hua
Kete Kimi Waikawa Karihi Ao HWTS-RT017A (PCR Whakapūngao)
Te mate urutā
Ko te adenovirus tangata (HAdV) nō te momo adenovirus Mammalian, he huaketo DNA aho-rua kāore he takai. Ko ngā adenovirus kua kitea tae noa mai ki tēnei wā ko ngā rōpū iti e 7 (AG) me ngā momo e 67, ā, e 55 ngā momo he mate ki te tangata. I roto i ēnei, ko ngā mate pea e arahi ana ki ngā mate manawa ko te rōpū B (Momo 3, 7, 11, 14, 16, 21, 50, 55), Rōpū C (Momo 1, 2, 5, 6, 57) me te Rōpū E (Momo 4), ā, ko ngā mate puku ko te Rōpū F (Momo 40 me te 41) [1-8]. He rerekē ngā tohu haumanu o ia momo, engari ko ngā mate manawa te nuinga. Ko ngā mate manawa e puta mai ana i ngā mate manawa o te tinana tangata e 5%~15% o ngā mate manawa o te ao, me te 5%-7% o ngā mate manawa o ngā tamariki o te ao [9]. He whānuitia te horapa o te adenovirus, ā, ka taea te pangia i te tau katoa, inā koa i ngā wāhi kī tonu i te tangata, e ngāwari ana te horapa o ngā mate urutā ā-rohe, inā koa i ngā kura me ngā puni hōia.
Hongere
| FAM | adenovirus whānuiwaikawa karihi |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Te ūkui ihu，Te ūkui korokoro |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Ngā Tārua/mL |
| Te motuhaketanga | a) Whakamātauria ngā tohutoro kino a te kamupene paerewa mā te kete, ā, ka tutuki i te hua whakamātautau ngā whakaritenga. b) Whakamahia tēnei kete hei kimi, ā, kāore he tauhohenga whakawhiti ki ētahi atu mate manawa (pēnei i te huaketo rewharewha A, te huaketo rewharewha B, te huaketo rewharewha syncytial, te huaketo parainfluenza, te huaketo rhinovirus, te huaketo metapneumovirus tangata, me ētahi atu) me ngā huakita rānei (streptococcus pneumoniae, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus, me ētahi atu). |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plusngā (FQD-96A, HangzhouHangarau Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Ngā Pūnaha PCR Wā-Tūturu BioRad CFX96, Ngā Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
(1) Te matū tango e taunakitia ana:Te Whakamātautau Tauira Macro me Micro-Test (HWTS-3005-8). Me mahi te tangohanga kia rite ki ngā tohutohu. Ko te tauira i tangohia ko ngā tūroro'He tauira ūkui ihu, he tauira ūkui korokoro rānei i kohia i te wāhi. Tāpirihia ngā tauira ki te matū tuku tauira nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kia pai te whakaranu, waiho ki te pāmahana rūma mō te 5 meneti, tangohia atu, kātahi ka hurihia ka whakaranu kia pai te whakaranu kia whiwhi ai i te DNA o ia tauira.
(2) Te matū tango e taunakitia ana:Whakamātautau-Makro me te Moroiti huaketo Kete DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).me mahi te mahi i runga anō i ngā tohutohu. Ko te rahinga o te tauira i tangohia he 200µL, me tete nui o te wairewa e taunakitia anais80μL.
(3) Te matū tango e taunakitia ana: Te matū tango waikawa karihi, te matū horoi rānei (YDP)315) nā Tiangen Biotech (Beijing) Co.,Ltd., teme whakahaere te mahi kia rite tonu ki ngā tohutohu. Ko te rōrahi tauira i tangohia he 200µL, me tete nui o te wairewa e taunakitia anais80μL.
