ALDH ira Polymorphism
ingoa hua
HWTS-GE015Kete Tirohanga Ira Polymorphism ALDH (ARMS -PCR)
Epidemiology
Ko te ira ALDH2 (acetaldehyde dehydrogenase 2), kei runga i te chromosome tangata 12. Kei te ALDH2 te mahi esterase, dehydrogenase me te reductase i te wa ano. Kua whakaatuhia e nga rangahau ko te ALDH2 he whaakiki metabolic o te nitroglycerin, e huri ana i te nitroglycerin ki te waikura nitric, na reira ka whakangawari i nga oko toto me te whakapai ake i nga mate rere toto. Heoi ano, he polymorphisms kei roto i te ira ALDH2, kei te Rawhiti o Ahia te nuinga. Ko te momo mohoao ALDH2 * 1 / * 1 GG he kaha te kaha o te tinana, ko te momo heterozygous he 6% noa iho o te momo mahi enzyme mohoao, a ko te momo mutant homozygous he tata kore te mahi enzyme, he ngoikore rawa te paopao, kaore e taea te whakatutuki i te paanga e hiahiatia ana, na reira ka kino te tinana o te tangata.
Hongere
| FAM | ALDH2 |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | EDTA toto anticoagulated |
| CV | <5.0％ |
| LoD | 103Nga kape/mL |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Nga tauhohenga tangohanga e taunakitia ana: whakamahia te Kete Tangohanga DNA Toto Genome DNA (DP318) na Tiangen Biotech (Beijing) Co., Ltd. ranei Blood Genome Extraction Kit (A1120) na Promega ki te tango EDTA anticoagulated toto Genomic DNA.
Ko nga reagents tangohanga e manakohia ana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me tino whakahaere te tangohanga kia rite ki nga tohutohu. Ko te rōrahi elution e tūtohu ana100μL.
