Te Whakapūtanga Ira ALDH
Ingoa hua
HWTS-GE015Kete Kimi i te Pūāhua Ira ALDH (ARMS -PCR)
Te mate urutā
Kei te ira tangata 12 te ira ALDH2 (acetaldehyde dehydrogenase 2). Kei te ALDH2 te mahi esterase, dehydrogenase me te reductase i te wā kotahi. Kua whakaatuhia e ngā rangahau ko te ALDH2 he whākōkī pākia o te nitroglycerin, e huri ana i te nitroglycerin hei hauota hauota, ā, mā reira ka whakangāwari i ngā oko toto me te whakapai ake i ngā mate rere toto. Heoi, he maha ngā momo rerekētanga i roto i te ira ALDH2, e kitea nuitia ana i Āhia ki te Rāwhiti. He kaha te kaha pākia o te momo-mohoao ALDH2*1/*1 GG, ko te momo-heterozygous he 6% noa iho o te mahi whākōkī momo-mohoao, ā, tata kore he mahi whākōkī o te momo-whakarerekē homozygous, he tino ngoikore te pākia o te pākia, ā, kāore e taea te whakatutuki i te hua e hiahiatia ana, ā, ka pā kino ki te tinana tangata.
Hongere
| FAM | ALDH2 |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Toto EDTA ārai totoka |
| CV | <5.0% |
| LoD | 103Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Ngā matū tango e taunakitia ana: whakamahia te Kete Tango Ira Ira Toto (DP318) nā Tiangen Biotech (Beijing) Co.,Ltd. rānei te Kete Tango Ira Ira Toto (A1120) nā Promega hei tango i te Ira Ira toto kua āraihia te toto EDTA.
Ngā matū tango e taunakitia ana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tango kia rite ki ngā tohutohu. Ko te rōrahi elution e taunakitia ana ko100μL.
