Ira Aatete Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
ingoa hua
HWTS-OT045 Carbapenem Ātete Ira (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kete Rapu (Fluorescence PCR)
Epidemiology
Ko nga rongoā paturopi Carbapenem he paturopi β-lactam atypical me te whanui o te awhi antibacterial me te kaha antibacterial mahi.Na tona pumau ki te β-lactamase me te iti o te paitini, kua noho ko ia tetahi o nga raau taero antibacterial tino nui mo te maimoatanga o nga mate huakita nui.Ko nga Carbapenems he tino pumau ki te plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, me te plasmid-mediated cephalosporinases (AmpC enzymes).
Hongere
| PCR-Ranu 1 | PCR-Ranu 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Mana o roto | Mana o roto |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Sputum, koroni parakore, swab rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Tauwhāiti | a) Ka kitea e te kete nga tohutoro kino a te kamupene paerewa, ka tutuki nga hua ki nga whakaritenga o nga tohutoro e rite ana. b) Ko nga hua o te whakamatautau-whakawhitinga e whakaatu ana karekau he tauhohenga whakawhiti o tenei kete ki etahi atu tukumate manawa, penei i te Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influentoza, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, he tauira ranei kei roto i etahi atu ira aukati tarukino CTX, mecA, SME, SHV, TEM. c) Anti-interference: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin kua tohua mo te whakamatautau pokanoa, a ko nga hua e whakaatu ana ko nga matū pokanoa kua whakahuahia ake nei kaore he raruraru. ki te kitenga o nga ira aukati carbapenem KPC, NDM, OXA48, OXA23, VIM, me IMP. |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd. ) LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A，HangzhouHangarau Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Reagent Tangohanga Manakohia: Tonotono me te Whakamatau Mokoiti General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Tāpirihia te 200μL o te saline noa ki te thallus precipitate.Ko nga mahi ka whai ake me whai i nga tohutohu mo te tangohanga, a ko te rahinga o te elution e taunaki ana100μL.
Kōwhiringa 2.
Reagent Tangohanga Manakohia: Tangohanga Acid Nucleic or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Me timata te tangohanga kia rite ki te taahiraa 2 o te tohutohu mo te whakamahi (taapirihia te 200μL o te buffer GA ki te thallus precipitate , ka wiri kia mutu ra ano te thallus).Whakamahia te wai kore utu RNase/DNase mo te elution, a ko te 100μL te rōrahi elution e taunaki ana.
Kōwhiringa 3.
Reagent tangohanga Manakohia: Tonotono & Moroiti-Whakamātautau Tauira Reagent Tuku.Me horoi te tauira sputum ma te taapiri i te 1mL o te tote noa ki te tote thallus kua whakahuahia i runga ake nei, ka werohia ki te 13000r/min mo te 5 meneti, ka makahia te supernatant (pupuri 10-20µL o te supernatant).Mo te koroni parakore me te ūkui tapau, tāpirihia te 50μL o te tauira tuku tauhohenga tika ki te thallus parapara kua whakahuahia i runga ake nei, a me tango nga mahi ka whai ake kia rite ki te tohutohu mo te whakamahi.
