Ko te ira o te Carbapenem Garbapenem (KPC / NDM / oxa 48 / oxa / vim / qu)
Ingoa Hua
HWSS-OT045 Carbapenem Gradence Arbapene (KPC / NDM / oxa 48 / oxa / vim / qim) Kite "
Manganga
Ko nga rongoā haumanu o te carbapenem he antibiotics anypical β-lactam me te whanui antibacteriach me te mahi antibactete kaha. Na te mea e pumau tonu ana ki te β-lactamase me te iti o te waikore, kua riro i tetahi o nga raau taero antibacterial tino nui mo te maimoatanga o nga mate mate pukupuku kino. He tino pumau nga Carbapenes ki nga plactrum e whai kiko ana-whaaia-lactamas (esbls), chromosomes, me te cephalosporinases (me te cephalosporinases.
Takiwā rerenga
| PCR-Mix | PCR-Mix | |
| Whānau | Whakariki | Hāmene |
| Vic / Hex | Te mana o roto | Te mana o roto |
| Cy5 | Ndm | Kpc |
| Rox | Oo48
| Oo23 |
Tohu hangarau
| Pākoro | ≤-18 ℃ |
| Te Haahi-Ora | 12 marama |
| Momo tauira | Sputum, nga koroni ora, Swab Swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Koropū | 103CFU / ML |
| Whakawhāititanga | a) Ka kitea e te kete nga tohu kino a te kamupene, me nga hua e tutuki ana i nga whakaritenga o nga tohutoro e rite ana. b) Nga hua o te whakamatautauranga whakawhiti-tauhohenga kaore he uruparenga o tenei kete ki etahi atu o nga pathiniel pneumonia, a Aiptherelocaches I roto i te rewharewha, a Actinesabacter Junii, a Actinelbacter Haemolyticus, legheylasla pneumophi, lesida aldicanonia, chlaida aldicaccus, chaslidashinias, me te tauira smev, sme, sme, sme, SMV, SME, SMV etc. c) Anti-wawaotanga: Mucin, Minocycline, Clindazone, Cefopycin, Cefoproflom, Ciprofloecin Hydrochloride, Ko nga hua kua tohua, ko Roxoflocin, ko Roxoflonic Tuhinga o mua Ko nga ira o Carbapenem Genes KPC, NDM, oxa48, oxa23, vim, me te kanga. |
| Nga taputapu e tika ana | Te tono Biosystems 7500 nga punaha PCR-tūturu Raupaparorohiko PCR-RealTudio®5 Slan-96p Real-96P tūturu (Hongshi Medical Hangarau Hangarau Co., Ltd.) Pūnaha PCR Realcycler®480 LINEGENE 9600 PLUL DATER TRACTRANGA PCR (FQD-96AKāore,HangzhouHangarau Bioer) Ma-6000 Motuhake Thermal Thermal-Time (Suzhou Molahy Co., Ltd.) Biorad CFX96 Pūnaha Real-Time Biorad CFX Opus 96 Pūnaha Real-Time |
Rere mahi
Kōwhiringa 1.
Whakahōu Whakawhiwhinga: Macro & Micro-whakamatautau General DNA / RNA Kit (HWS-3019-50, HWS-3019-32, HWS-3019-48, HWS-3019-96) (ka taea te whakamahi me te macro & te miihini-3006c, hwts-3006b)) na Jiangsu Macro & Midd-Orfory Med ... tāpiri 200μl o te tote noa ka raru te thallus. Ko nga mahi e whai ake nei me whai i nga tohutohu mo te tangohanga, me te rōnakitanga e taunakitia ana100μl.
Kōwhiringa 2.
Te Whakahoki Whakawhiwhinga: Te tangohanga waikawa Nuku, te Whakatutuhia ranei (Beijing) a Tiangen Biotech (Beijing) Comper , ka wiri tae noa ki te mea kua tino parea katoatia te thalelus). Whakamahia te Rnase / DNASE FREE Mo Te Maramatanga, me te kohinga Econdecksing Ertive ko 100μl.
Kōwhiringa 3.
Whakahoutanga Tangohanga: Macro & Whakamatau tauira tauira-whakamatautau. Me horoi te tauira sputum ma te taapiri i te 1ml o te tote-a-tau i whakahuatia ake nei, i te 13000r / min mo te 5 meneti, a ka whakakorea te supernatant (pupuri 10 garnatant). Mo te koroni parakore me te papa tapawha, whakauruhia te 50μl o te tauira tuku i runga ake nei, me neke atu i nga waahanga o muri mai i te tohutohu mo te whakamahi.
