Ira Ātete Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Ingoa hua
Kete Kimi ira Ātete Carbapenem HWTS-OT045 (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (PCR Whakapūngao)
Te mate urutā
Ko ngā paturopi Carbapenem he paturopi β-lactam rerekē, he whānui te whānuitanga o ngā paturopi me te kaha paturopi kaha rawa atu. Nā tōna pumau ki te β-lactamase me te iti o te paitini, kua noho ko tētahi o ngā rongoā paturopi tino nui mō te rongoā i ngā mate huakita kino. He tino pumau ngā Carbapenem ki ngā β-lactamases whānui-arotahi (ESBL), ngā chromosomes, me ngā cephalosporinases (ngā whākōkī AmpC) e arohia ana e te plasmid.
Hongere
| PCR-Ranu 1 | PCR-Ranu 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Mana Whakahaere ā-roto | Mana Whakahaere ā-roto |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Hupa, koroni parakore, ūkui rectum |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Te motuhaketanga | a) Ka kitea e te kete ngā tohutoro kino a te kamupene kua whakaritea, ā, ka tutuki i ngā hua ngā whakaritenga o ngā tohutoro e pā ana. b) E whakaatu ana ngā hua o te whakamātautau tauhohenga-whakawhiti kāore he tauhohenga-whakawhiti o tēnei kete ki ētahi atu mate manawa, pērā i te Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, ngā tauira rānei kei roto ko ētahi atu ira ātete-rongoa CTX, mecA, SME, SHV, TEM, me ētahi atu. c) Ārai-whakararuraru: Ko Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin ngā mea kua tīpakohia mō te whakamātautau whakararuraru, ā, e whakaatu ana ngā hua kāore he tauhohenga whakararuraru o ngā matū whakararuraru kua whakahuatia ake nei ki te kimi i ngā ira ātete carbapenem arā, arā, a KPC, NDM, OXA48, OXA23, VIM, me IMP. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A，HangzhouHangarau Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Pūmua tango e taunakitia ana: Kete DNA/RNA Whānui Macro & Micro-Test (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Tāpirihia he 200μL o te tote noa ki te matū thallus. Me whai ngā mahi e whai ake nei i ngā tohutohu mō te tango, ā, ko te rōrahi elution kua tūtohutia ko100μL.
Kōwhiringa 2.
Te matū tango e taunakitia ana: Te matū tango waikawa karihi, te matū horoi rānei (YDP302) nā Tiangen Biotech (Beijing) Co., Ltd. Me tīmata te tango i runga anō i te taahiraa 2 o te tohutohu whakamahi (tāpirihia he 200μL o te GA pūmau ki te matū thallus, ka rūrū kia tau katoa ngā thallus). Whakamahia te wai kore RNase/DNase mō te elution, ā, ko te rōrahi elution e taunakitia ana he 100μL.
Kōwhiringa 3.
Te matū tango e taunakitia ana: Te matū tuku tauira Macro & Micro-Test. Me horoi te tauira huware mā te tāpiri i te 1mL o te tote noa ki te matū thallus kua rongoatia i runga ake nei, ka hurihia ki te 13000r/min mō te 5 meneti, kātahi ka maka atu te waikeri (pupurihia te 10-20µL o te waikeri). Mō te koroni parakore me te ūkui rectum, tāpirihia te 50μL o te matū tuku tauira tika ki te matū thallus kua rongoatia i runga ake nei, ā, me tango ngā mahi e whai ake nei kia rite ki te tohutohu whakamahi.
