E waru nga ahua o nga huaketo respiratory
ingoa hua
HWTS-RT184-E waru nga ahua o nga huaketo rewharewha i te Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te mate rewharewha te momo mate o te tangata, ka puta mai i nga ira tangata, i nga tau me nga rohe, a ko tetahi o nga tino take o te mate me te mate o te ao.[1]. Ko nga tukumate rewharewha noa i te haumanu ko te rewharewha A (IFV A), te rewharewha B (IFV B), te rewharewha syncytial virus, te adenovirus, te tangata metapneumovirus, te rhinovirus, te parainfluenza virus (I/II/III) me te mycoplasma pneumoniae, etc.[2,3]. He rite tonu nga tohu haumanu me nga tohu i puta mai i te rewharewha, engari ko te mate na nga momo tukumate rereke he rereke nga tikanga maimoatanga, nga hua whakaora me te ahua o te mate.[4,5]. I tenei wa, ko nga tikanga matua mo te tirotiro taiwhanga o nga tukumate rewharewha ko: te wehenga huaketo, te kitenga antigen me te kimi waikawa nucleic. Ka kitea, ka tautuhia e tenei kete nga waikawa nucleic viral motuhake i roto i nga tangata takitahi me nga tohu me nga tohu o te mate manawa, i te taha o etahi atu huanga haumanu me te taiwhanga hei awhina i te tohu o te mate rewharewha.
Tawhā Hangarau
| Rokiroki | 2-8 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te paninga oropharyngeal; Puka nasopharyngeal |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 kape/mL |
| Tauwhāiti | Whakahohenga whakawhiti: Karekau he tauhohenga whakawhiti i waenga i te kete me te huaketo Boca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, wheori karawaka, coronavirus tangata, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptoellacoccus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Klebsigene Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobaclicus DNA, Corynebaclicus DNA. Whakamātautau pokanoa: Tīpakohia te mucin (60mg/mL), toto tangata (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), konutai pūhaumāota me te 5% te tiaki (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL) (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpirocin (20mg/mL), mucinpirocin (20mg/mL) oseltamivir (60ng/mL), ribavirin (10mg/L), Ko nga hua i whakaatu ko nga matū pokanoa i te concentra o runga |
| Nga taputapu e tika ana | E tika ana ki te reagent rapunga momo I: Applied Biosystems 7500 Pūnaha PCR Wā-tūturu, QuantStudio®5 Pūnaha PCR Wā-tūturu, SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangarau Hangzhou Bioer), MA-6000 Waa Tuturu Ine Waahanga Hukarere Ngawha (Suzhou Molarray Real Time CFX96), Pūnaha BioRad FX96. Opus 96 Pūnaha PCR Wā-tūturu. E tika ana ki te momo II reagent rapunga: EudemonTM AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Rere Mahi
Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), me te Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8 ka taea te whakamahi me Euch)TM AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ko 200μL te rōrahi tauira tangohia me te rōrahi elution tūtohu ko 150μL.
