Enterovirus Universal, EV71 me CoxA16
Ingoa Hua
HWTS-EV026B-Enterovirus Universal, EV71 me CoxA16 Kete Rapu Waikawa Nucleic(Fluorescence PCR)
HWTS-EV020Y/Z-Enterovirus Universal, EV71 me te CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
Tiwhikete
CE/MDA（HWTS-EV026）
Epidemiology
Ko te mate ringa-waewae-waha (HFMD) he mate hopuhopu whakapeka noa i roto i nga tamariki. Ko te nuinga ka pa ki nga tamariki kei raro iho i te 5 tau te pakeke, a ka mate pea te mate herpes i runga i nga ringa, waewae, waha me etahi atu waahanga, a he iti te maha o nga tamariki ka puta he raruraru penei i te myocarditis, pulmonary edema, aseptic meningoencephalitis, me etahi atu.
I tenei wa, kua kitea 108 serotypes o enteroviruses, e wehewehea ana ki nga roopu e wha: A, B, C me D. Ko nga enteroviruses e puta ai te HFMD he rereke, engari ko te enterovirus 71 (EV71) me te coxsackievirus A16 (CoxA16) ko te mea tino noa me te taapiri atu ki te HFMD, ka taea e te mate te mate o te punaha o te puku, te paralysis me te pararutiki o te punaha.
Hongere
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Mana whakahaere o roto |
Tawhā Hangarau
| Rokiroki | Wai: ≤-18 ℃ I te pouriLyophilization: ≤30 ℃ |
| Papa-ora | Wai: 9 maramaLyophilization: 12 marama |
| Momo Tauira | He tauira mo te korokoro, te wai Herpes |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Kape/mL |
| Nga taputapu e tika ana | Ka taea e ia te rite ki nga taputapu PCR fluorescent i runga i te maakete.ABI 7500 Pūnaha PCR Wā-tūturu ABI 7500 Pūnaha PCR Wā-tūturu Tere SLAN-96P Pūnaha PCR Wā-tūturu QuantStudio®5 Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Tapeke PCR Solution
Reagent tangohanga Manakohia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ka taea te whakamahi me te Tohutono & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tangohanga kia rite ki te tohutohu. Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 80μL.
Reagent tangohanga Manakohia: Tonotono & Moroiti-Whakamātautau Tauira Reagent Tuku (HWTS-3005-8). Me whakahaere te tangohanga kia rite ki te pukapuka tohutohu. Ko nga tauira tangohanga he oropharyngeal swabs, he tauira wai herpes ranei o nga turoro i kohia ki te waahi. Taapirihia nga tapahi kua kohia ki te Tohutono & Micro-Test Sample Reagent Reagent, vortex me te uru pai, tuu ki te pāmahana rūma mo te 5 meneti, tangohia, ka huri, ka uru pai kia whiwhi te RNA o ia tauira.
Reagent tangohanga Manakohia: QIAamp Viral RNA Mini Kit (52904) na QIAGEN, Nucleic Acid Extraction ranei Purification Reagent (YDP315-R). Ko te tangohanga me tino rite ki te pukapuka tohutohu.
