Enterovirus Universal, EV71 me CoxA16
Ingoa Hua
Kete Kimi Waikawa Karihi HWTS-EV026B-Enterovirus Ao Whānui, EV71 me CoxA16 (PCR Whakaata)
HWTS-EV020Y/Z-Kete Kimi Waikawa Nucleic Enterovirus Universal, EV71 me CoxA16 kua maroke-tio (PCR Fluorescence)
Tiwhikete
CE/MDA（HWTS-EV026）
Te mate urutā
Ko te mate ringa-waewae-waha (HFMD) he mate hopuhopu nui noa i roto i ngā tamariki. Ko te nuinga o te wā ka pā ki ngā tamariki kei raro iho i te 5 tau te pakeke, ā, ka pāngia e te herpes ki ngā ringa, waewae, waha me ētahi atu wāhanga, ā, ka pāngia pea e te iti o ngā tamariki ngā raruraru pērā i te myocarditis, te pulmonary edema, te aseptic meningoencephalitis, me ētahi atu. Ka tere te heke o te kaha o te mate o ia tamaiti me ngā mate kino, ā, ka mate pea mēnā kāore e rongoatia wawetia.
I tēnei wā, e 108 ngā momo enterovirus kua kitea, e wehea ana kia whā ngā rōpū: A, B, C me D. He rerekē ngā enterovirus e puta ai te HFMD, engari ko te enterovirus 71 (EV71) me te coxsackievirus A16 (CoxA16) ngā mea tino kitea, ā, hei tāpiri atu ki te HFMD, ka taea e rātou te puta he raruraru nui ki te pūnaha io matua pērā i te meningitis, te encephalitis, me te acute flaccid paralysis.
Hongere
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Mana whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | Wai: ≤-18℃ I te pōuriTe whakapūmautanga o te toto: ≤30℃ |
| Te roa o te oranga | Wai: 9 maramaTe whakapūmautanga o te toto: 12 marama |
| Momo Tauira | Tauira ūkui korokoro, wai Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Ka taea e ia te taurite ki ngā taputapu PCR hihiko matua kei te mākete.Ngā Pūnaha PCR Wā-Tūturu ABI 7500 Ngā Pūnaha PCR Tere Wā-Tūturu ABI 7500 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu LightCycler®480 Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Otinga PCR Katoa
Te matū tango e taunakitia ana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me mahi te tango kia rite ki te pukapuka tohutohu. Ko te rahinga tauira i tangohia he 200μL, ā, ko te rahinga wairewa e taunakitia ana he 80μL.
Te matū tango e taunakitia ana: Te matū tuku tauira Macro & Micro-Test (HWTS-3005-8). Me mahi te tango kia rite ki te pukapuka tohutohu. Ko ngā tauira tango he tauira ūkui o te waha, he tauira wai herpes rānei o ngā tūroro i kohia i te wāhi. Tāpirihia ngā ūkui i kohia tika ki te matū tuku tauira Macro & Micro-Test, vortex ka whakaohokia kia pai, waiho ki te pāmahana rūma mō te 5 meneti, tangohia atu, kātahi ka hurihia ka whakaohokia kia pai kia whiwhi ai i te RNA o ia tauira.
Te matū tango e taunakitia ana: QIAamp Viral RNA Mini Kit (52904) mā te QIAGEN, te matū tango waikawa nucleic rānei, te matū horoi rānei (YDP315-R). Me whakahaere te tango kia rite tonu ki te pukapuka tohutohu.
