Huaketo Encephalitis Ngāhere
Ingoa hua
Kete Kimi Waikawa Matū HWTS-FE006 mō te Huaketo Encephalitis Ngāhere (PCR Fluorescence)
Te mate urutā
Ko te mate pukupuku ngahere (FE), e mōhiotia ana ko te mate pukupuku kawe-tiki (Tick-borne encephalitis, TBE), he mate hopuhopu whakapeka o te pūnaha io matua i puta mai i te huaketo mate pukupuku ngahere. Nō te puninga Flavivirus o te whānau Flaviviridae te huaketo mate pukupuku ngahere. He porowhita ngā matūriki huaketo, ā, he 40-50nm te whānui. Ko te taumaha ngota he tata ki te 4×106Da, ā, ko te ira huaketo he RNA kotahi-aho, he pai te tikanga[1]. I roto i te āhua haumanu, ko te kirika nui, te ānini, te koma, te tere o te mumura o te meningeal, me te pararutiki o ngā uaua o te kaki me ngā peka, ā, he nui te mate. Ko te tautuhi wawe me te tere o te huaketo encephalitis ngahere te mea nui ki te rongoā i te encephalitis ngahere, ā, ko te whakatū i tētahi tikanga tautuhi māmā, motuhake me te tere o te take he mea nui ki te tautuhi haumanu o te encephalitis ngahere.[1,2].
Hongere
| FAM | huaketo encephalitis ngahere waikawa karihi |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | serum hou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Ko te matū tango e taunakitia ana: Kete Tango Waikawa Nucleic (YDP315-R) nā Tiangen Biotech (Beijing) Co.,Ltd., me whakahaere te tango kia rite ki ngā tohutohu. Ko te rōrahi tauira tango e taunakitia ana ko 140μL, ā, ko te rōrahi wairewa e taunakitia ana ko 60μL.
Te matū tango e taunakitia ana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Me whakahaere te tango kia rite ki ngā tohutohu. Ko te rōrahi tauira tango e taunakitia ana ko te 200μL, ā, ko te rōrahi wairewa e taunakitia ana ko te 80μL.
