Ngahere Encephalitis Virus
ingoa hua
HWTS-FE006 Ngahere Encephalitis Virus Huori Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Ngahere encephalitis (FE), e mohiotia ana ko tick-borne encephalitis (Tick-borne encephalitis, TBE), he mate hopuhopu whakapeka o te punaha io matua na te huaketo encephalitis ngahere. Ko te huaketo encephalitis ngahere no te puninga Flavivirus o te whanau Flaviviridae. Ko nga matūriki huaketo he porowhita me te diameter o te 40-50nm. Ko te taumaha irapoi mo te 4×106Da, me te ira tangata wheori he pai-whakaaro, RNA pae kotahi[1]. I runga i te haumanu, he nui te kirikaa, he mahunga, he koma, he tere te tiimata o te mate pukupuku, me te pararutiki uaua o te kaki me nga peka, he nui te mate. Ko te whakamaarama wawe me te tere o te huaketo encephalitis ngahere te mea nui ki te maimoatanga o te ngahere encephalitis.[1,2].
Hongere
| FAM | ngahere encephalitis huaketo waikawa nucleic |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 9 marama |
| Momo Tauira | serum hou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Kape/mL |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent tangohanga Manakohia: Tangohanga Acid Nucleic ranei Kit Purification (YDP315-R) na Tiangen Biotech(Beijing) Co., Ltd., me whakahaere te tangohanga kia rite ki nga tohutohu. Ko te 140μL te rahinga tauira kua tangohia ko te 60μL te rōrahi elution tūtohutia.
Reagent tangohanga Manakohia: Tohutono & Moroiti-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-6C-306) HWTS-3006B). me whakahaere te tangohanga kia rite ki nga tohutohu. Ko te rōrahi tauira kua tangohia ko te 200μL me te rōrahi elution tūtohu ko 80μL.
