Chlamydia Trachomatis kua maroke-tio
Ingoa hua
HWTS-UR032C/D-Kete Kimi Waikawa Nucleic Chlamydia Trachomatis kua Maroke-tio (Whakanuia te Whakamātautau Enzymatic Isothermal)
Te mate urutā
Ko te Chlamydia trachomatis (CT) he momo moroiti prokaryotic e pirinoa ana i roto i ngā pūtau eukaryotic.[1]. Kua wehea te Chlamydia trachomatis ki ngā momo AK e ai ki te tikanga momo. Ko ngā mate pūkahukahu e puta mai ana i ngā momo DK rerekē o te trachoma te nuinga o te wā, ā, ko ngā tāne ka kitea ko te urethritis, ka taea te whakaora me te kore rongoā, engari ko te nuinga ka roa, ka kino haere i ētahi wā, ā, ka taea te hono atu ki te epididymitis, te proctitis, me ētahi atu.[2]Ka pāngia pea ngā wāhine e te urethritis, te cervicitis, me ētahi atu, me ngā raruraru kino atu o te salpingitis.[3].
Hongere
| FAM | Chlamydia trachomatis (CT) |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤30℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Te ūkui waha o te wahine Te ūkui urethral tāne Mimi tāne |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Ngā Tārua/mL |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti i waenga i tēnei kete me ētahi atu mate pāwera pūnaha urogenital pērā i te Human papillomavirus momo 16, Human papillomavirus momo 18, Herpes simplex virus momo Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei me te human genomic DNA, me ētahi atu. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 me te Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 Pūnaha Kimi Whakapūmau Hikohiko Wā-tūturu Māmā（HWTS-1600）. |
Rere Mahi
Kōwhiringa 1.
Te Tauhohenga Tukunga Tauira Macro me Micro-Test (HWTS-3005-8). Me whakahaere te tangohanga i runga i te tino rite ki te IFU. Tāpirihia te DNA tauira i tangohia e te tauhohenga tukunga tauira ki roto i te pūwero tauhohenga ka whakamātautau tika ki te taputapu, ki te kore, me penapena ngā tauira i tangohia ki te 2-8℃ mō te kore e neke atu i te 24 hāora.
Kōwhiringa 2.
Kete Whakamātautau Ira/RNA Whānui (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Me whakahaere te tangohanga i runga i te tino rite ki te IFU, ā, ko te rōrahi elution e taunakitia ana ko te 80μL. Ka whakamahanahia te DNA tauira i tangohia mā te tikanga aukume ki te 95°C mō te 3 meneti, kātahi ka kaukau tonu ki te hukapapa mō te 2 meneti. Tāpirihia te DNA tauira kua tukatukahia ki roto i te pūwero tauhohenga ka whakamātautau ki runga i te taputapu, ki te kore, me penapena ngā tauira kua tukatukahia ki raro i te -18°C mō te kore e neke atu i te 4 marama. Kaua e neke atu i te 4 huringa te maha o te tio me te whakarewatanga.
