Ko Chlamydia Trachomatis kua whakamarokehia
ingoa hua
HWTS-UR032C/D-Ko Chlamydia Trachomatis kua whakamaroke-maroke i te Kete Rapu Waikawa Nucleic (Enzymatic Probe Isothermal Amplification)
Epidemiology
Ko Chlamydia trachomatis (CT) he momo moroiti prokaryotic he tino parapara i roto i nga pūtau eukaryotic.[1].Ka wehewehea a Chlamydia trachomatis ki nga AK serotypes i runga i te tikanga serotype.Ko te nuinga o nga mate o te urogenital i ahu mai i nga momo momo DK rerekee o te trachoma, a ko te nuinga o nga tane ka kitea ko te urethritis, ka taea te rongoa me te kore e rongoa, engari ko te nuinga o te hunga ka mau tonu, ka kaha haere i ia wa, ka taea te whakakotahi ki te epididymitis, proctitis, etc.[2].Ko nga wahine ka pa mai ki te urethritis, cervicitis, aha atu, me etahi atu mate kino o te salpingitis.[3].
Hongere
| FAM | Chlamydia trachomatis (CT) |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤30 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te uwhi uwha wahine ūkui urethral tane Mimi tane |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 kape/mL |
| Tauwhāiti | karekau he tauhohenga whakawhiti i waenga i tenei kete me etahi atu mate tukumate i roto i te taihemahema penei i te momo mate papillomavirus tangata 16, momo papillomavirus tangata 18, momo huaketo Herpes simplex Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylodiscoccus , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei me te DNA genomic tangata, etc. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu(Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu me BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu Ngawari Amp Pūnaha Tirohanga Isothermal Waa-Tuturu（HWTS-1600）. |
Rere Mahi
Kōwhiringa 1.
Tonotono & Moroiti-Whakamātautau Tauira Tuku Reagent (HWTS-3005-8).Ko te tangohanga me whakahaere kia rite ki te IFU.Tāpirihia te tauira DNA i tangohia e te tauira tuku tauhohenga ki roto i te parepare tauhohenga me te whakamatautau tika ki te taputapu, me penapena ranei nga tauira kua tangohia ki te 2-8 ℃ mo te kore neke atu i te 24 haora.
Kōwhiringa 2.
Tonotono & Moroiti-Whakamātautau Kete DNA/RNA Whānui (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Me whakahaere te tangohanga i roto i te tino rite ki te IFU, a ko te 80μL te rōrahi elution tūtohu.Ko te tauira DNA i tangohia mai i te tikanga pirepire autō ka whakamahana ki te 95°C mo te 3 meneti, katahi ka horoi tonu i te huka mo te 2 meneti.Tāpirihia te tauira DNA kua tukatukahia ki roto i te parepare tauhohenga me te whakamatautau ki te taputapu, me pupuri ranei nga tauira kua tukatukahia ki raro -18°C mo te kore neke atu i te 4 marama.Kia kaua e neke ake i te 4 nga huringa te maha o te tio me te whakamaroke.
