Ko Chlamydia Trachomatis kua whakamarokehia
ingoa hua
HWTS-UR032C/D-Ko Chlamydia Trachomatis kua whakamaroke-maroke i te Kete Rapu Waikawa Nucleic (Enzymatic Probe Isothermal Amplification)
Epidemiology
Ko Chlamydia trachomatis (CT) he momo moroiti prokaryotic he tino parapara i roto i nga pūtau eukaryotic.[1]. Ka wehewehea a Chlamydia trachomatis ki nga AK serotypes i runga i te tikanga serotype. Ko te nuinga o nga mate o te urogenital i ahu mai i nga momo momo DK rerekee o te trachoma, a ko te nuinga o nga tane ka kitea ko te urethritis, ka taea te rongoa me te kore e rongoa, engari ko te nuinga o te hunga ka mau tonu, ka kaha haere i ia wa, ka taea te hono atu ki te epididymitis, proctitis, etc.[2]. Ko nga wahine ka pa mai ki te urethritis, cervicitis, aha atu, me etahi atu mate kino o te salpingitis.[3].
Hongere
| FAM | Chlamydia trachomatis (CT) |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤30 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te uwhi uwha wahine ūkui urethral tane Mimi tane |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 kape/mL |
| Tauwhāiti | karekau he tauhohenga whakawhiti i waenga i tenei kete me etahi atu mate tukumate o te waahanga taihemahema penei i te momo papillomavirus tangata morearea nui 16, momo papillomavirus tangata 18, momo huaketo Herpes simplex Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus, Epidermis, Epidermis. Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei me te DNA genomic tangata, etc. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu(Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu me BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu Ngawari Amp Pūnaha Tirohanga Isothermal Waa-Tuturu（HWTS-1600）. |
Rere Mahi
Kōwhiringa 1.
Tonotono & Moroiti-Whakamātautau Tauira Tuku Reagent (HWTS-3005-8). Ko te tangohanga me whakahaere kia rite ki te IFU. Tāpirihia te tauira DNA i tangohia mai e te tauira tuku tauhohenga ki roto i te parepare tauhohenga me te whakamatautau tika i te taputapu, me pupuri ranei nga tauira kua tangohia ki te 2-8 ℃ mo te kore neke atu i te 24 haora.
Kōwhiringa 2.
Tonotono & Moroiti-Whakamātautau Kete DNA/RNA Whānui (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Me whakahaere te tangohanga i roto i te tino rite ki te IFU, a ko te 80μL te rōrahi elution tūtohu. Ko te tauira DNA i tangohia mai i te tikanga pirepire autō ka whakamahana ki te 95°C mo te 3 meneti, katahi ka horoi tonu i te huka mo te 2 meneti. Tāpirihia te tauira DNA kua tukatukahia ki roto i te parepare tauhohenga me te whakamatautau ki te taputapu, me pupuri ranei nga tauira kua tukatukahia ki raro -18°C mo te kore neke atu i te 4 marama. Kia kaua e neke ake i te 4 nga huringa te maha o te tio me te whakamaroke.
