Hepatitis A Virus
ingoa hua
HWTS-HP005 Hepatitis A Virus Te Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Hepatitis A huaketo (HAV) Ko te tino take o te mate kakā viral whakapeka.Ko te wheori he huaketo RNA kotahi-waa pai, no te puninga Hepadnavirus o te whanau Picornaviridae.Ko te mate Hepatitis A, ko te nuinga ka tukuna ma te ara fecal-waha, he atete ki te wera, ki te waikawa, me te nuinga o nga whakarewa pararopi, ka ora mo te wa roa i roto i te maataitai, i te wai, i te oneone, i nga parataiao takere moana[1-3].Ka tukuna ma te kai poke, te wai ranei, ka tuku tika mai i te tangata ki te tangata.Ko nga kai e pa ana ki te HAV ko te tio me te pipi, te rōpere, te raspberries, te blueberries, te ra, te huawhenua rau matomato, me te tōmato āhua-maroke[4‒6].
Hongere
| FAM | HAV waikawa karihi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | Wai: 9 marama, Lyophilized: 12 marama |
| Momo Tauira | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Tārua/μL |
| Tauwhāiti | Whakamahia nga kete ki te whakamatautau i etahi atu mate pukupuku penei i te mate hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV me te genome tangata.Kare he tauhohenga whakawhiti. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-Tuturu (FQD-96A, Hangarau Hangarau Hangzhou Bioer), MA-6000 Waa-Tuturu Ine Hukarere Ngaariki BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Nga tauira serum
Kōwhiringa 1.
Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Me tango kia rite ki nga tohutohu.Ko te 80µL te rahinga o te elution.
Kōwhiringa 2.
TIANamp Virus DNA/RNA Kit (YDP315-R) i hangaia e Tiangen Biotech (Beijing) Co., Ltd. Me tango kia rite ki nga tohutohu.Ko te rahinga tauira kua tangohia he 140μL.Ko te rōrahi hauhā e tūtohu ana ko te 60µL.
2.Tauira kuhu
Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Me tango kia rite ki nga tohutohu.Ko te 80µL te rahinga o te elution.
