Hepatitis B Virus Genotyping
ingoa hua
HWTS-HP002-Hepatitis B Virus Te Kete Rapu Tohua (PCR)
Epidemiology
Epidemiology
Hongere
| HongereIngoa | Tauhohenga Kaipupuri 1 | Tauhohenga Pūreirei 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ I te pouri |
| Papa-ora | 12 marama |
| Momo Tauira | Serum, Plasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 1×102IU/mL |
| Tauwhāiti | Karekau he tauhohenga whakawhiti ki te huaketo hepatitis C, te cytomegalovirus tangata, te huaketo Epstein-Barr, te huaketo immunodeficiency tangata, te mate hepatitis A, te syphilis, te huaketo herpes, te huaketo rewharewha A, te propionibacterium acnes (PA), etc. |
| Nga taputapu e tika ana | Ka taea e ia te rite ki nga taputapu PCR fluorescent i runga i te maakete. ABI 7500 Pūnaha PCR Wā-tūturu ABI 7500 Pūnaha PCR Wā-tūturu Tere SLAN-96P Pūnaha PCR Wā-tūturu QuantStudio®5 Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Reagent tangohanga Manakohia: Tohutono & Micro-Test Viral DNA / RNA Kit (HWTS-3017) ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tangohanga i runga i te pukapuka o te tangohanga, kia rite ki te pukapuka tangohanga.200μL, me te rōrahi elution tūtohu ko80μL.
Reagent tangohanga e taunakihia ana: Tangohanga Acid Nucleic, Reagent Purification ranei (YDP315). Ko te tangohanga me tino rite ki nga tohutohu. Ko te ruri tauira kua tangohia ko te 200µL, me te 100µL te rōrahi elution e taunaki ana.
