Hepatitis E Virus
ingoa hua
HWTS-HP006 Hepatitis E Virus Te Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te mate Hepatitis E (HEV) he huaketo RNA e raru ai te hauora o te ao. He whanui te awhe kaihautu me te whai waahi ki te whakawhiti i nga arai mokowhiti. Koia tetahi o nga tukumate zoonotic tino nui ka pa te kino ki te tangata me te kararehe. Ko te HEV te nuinga o te tuku na roto i te tuku fecal-waha, ka taea hoki te tuku poutū ma roto i nga kukune, toto ranei. I roto i a raatau, i roto i te huarahi whakawhiti fecal-waha, ka horahia te wai me te kai kua pokea HEV, he nui te mate o te mate HEV ki te tangata me te kararehe [1-2].
Hongere
| FAM | HEV waikawa karihi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te korokoro |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kape/μL |
| Tauwhāiti | Ko te mate Hepatitis E (HEV) he huaketo RNA e raru ai te hauora o te ao. He whanui te awhe kaihautu me te whai waahi ki te whakawhiti i nga arai mokowhiti. Koia tetahi o nga tukumate zoonotic tino nui ka pa te kino ki te tangata me te kararehe. Ko te HEV te nuinga o te tuku na roto i te tuku fecal-waha, ka taea hoki te tuku poutū ma roto i nga kukune, toto ranei. I roto i a raatau, i roto i te huarahi whakawhiti fecal-waha, ka horahia te wai me te kai kua pokea HEV, he nui te mate o te mate HEV ki te tangata me te kararehe [1-2]. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Tere Pūnaha PCR Wā-tūturu QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1
Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006C). Me tango kia rite ki nga tohutohu. Ko te 80µL te rahinga o te elution.
Kōwhiringa 2
TIANamp Virus DNA/RNA Kit (YDP315-R) i hangaia e Tiangen Biotech (Beijing) Co., Ltd. Me tino tangohia kia rite ki nga tohutohu. Ko te rahinga tauira kua tangohia he 140μL. Ko te rōrahi hauhā e tūtohu ana ko te 60µL.v
