Huaketo Hepatitis E
Ingoa hua
Kete Kimi Waikawa Matū HWTS-HP006 mō te Huaketo Hepatitis E (PCR Whakapūngao)
Te mate urutā
Ko te huaketo Hepatitis E (HEV) he huaketo RNA e whakararuraru ana i te hauora o te ao. He whānui tōna awhe manaaki, ā, he āhuatanga tōna ki te whiti i ngā arai i waenga i ngā momo. Ko tētahi o ngā mate urutā nui rawa atu, ā, he nui te kino ki te tangata me te kararehe. Ko te nuinga o te wā ka horapa te HEV mā te tuku paru-waha, ā, ka taea hoki te tuku poutū mā te kukune, te toto rānei. I roto i ēnei, i te ara tuku paru-waha, ka horapa whānuitia te wai me te kai kua poke i te HEV, ā, he nui te mōrearea o te mate HEV i roto i te tangata me te kararehe [1-2].
Hongere
| FAM | Waikawa karihi HEV |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Te ūkui korokoro |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/μL |
| Te motuhaketanga | Ko te huaketo Hepatitis E (HEV) he huaketo RNA e whakararuraru ana i te hauora o te ao. He whānui tōna awhe manaaki, ā, he āhuatanga tōna ki te whiti i ngā arai i waenga i ngā momo. Ko tētahi o ngā mate urutā nui rawa atu, ā, he nui te kino ki te tangata me te kararehe. Ko te nuinga o te wā ka horapa te HEV mā te tuku paru-waha, ā, ka taea hoki te tuku poutū mā te kukune, te toto rānei. I roto i ēnei, i te ara tuku paru-waha, ka horapa whānuitia te wai me te kai kua poke i te HEV, ā, he nui te mōrearea o te mate HEV i roto i te tangata me te kararehe [1-2]. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Pūnaha PCR Tere-Tūturu a Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1
Kete Whakamātautau Ira Whānui mō te Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Me tango kia rite ki ngā tohutohu. Ko te rōrahi elution e taunakitia ana ko te 80µL.
Kōwhiringa 2
Kete DNA/RNA Huakita TIANamp (YDP315-R) i hangaia e Tiangen Biotech (Beijing) Co., Ltd. Me tango kia rite ki ngā tohutohu. Ko te rahinga tauira i tangohia he 140μL. Ko te rahinga wairewa i tūtohutia he 60µL.v
