Huaketo Rewharewha A Ao Whānui/H1/H3
Ingoa hua
Kete Kimi Waikawa Matū HWTS-RT012 mō te Huaketo Rewharewha A (PCR)
Te mate urutā
Ko te huaketo rewharewha he momo māngai o Orthomyxoviridae. He mate urutā e tino mōrearea ana ki te hauora o te tangata. Ka taea e ia te pangia te tinana e te mate urutā. E pāngia ana e te mate urutā wā te 600 miriona tāngata puta noa i te ao, ā, e 250,000 ~500,000 ngā mate, ko te huaketo rewharewha A te tino take o te pangia me te mate. Ko te huaketo rewharewha A he RNA kotahi-aho, he aho-kino. E ai ki tōna mata hemagglutinin (HA) me te neuraminidase (NA), ka taea te wehewehe i te HA ki ngā momo 16, NA Wehea ki ngā momo 9. I roto i ngā huaketo rewharewha A, ko ngā momo huaketo rewharewha ka pangia tika te tangata ko: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 me te H10N8. I roto i ēnei, he tino mate ngā momo H1 me te H3, ā, he mea tika kia arohia.
Hongere
| FAM | rewharewha momo whānui A waikawa karihi |
| VIC/HEX | huaketo rewharewha A momo H1 waikawa karihi |
| ROX | huaketo rewharewha A momo H3 waikawa karihi |
| CY5 | mana whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | ūkui ihu |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/μL |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti ki ētahi atu tauira manawa pērā i te rewharewha A, te rewharewha B, te Legionella pneumophila, te Rickettsia Q fever, te Chlamydia pneumoniae, te Adenovirus, te Respiratory Syncytial Virus, te Parainfluenza 1, 2, 3, te Coxsackie virus, te Echo virus, te Metapneumovirus A1/A2/B1/B2, te Respiratory syncytial virus A/B, te Coronavirus 229E/NL63/HKU1/OC43, te Rhinovirus A/B/C, te Boca virus 1/2/3/4, te Chlamydia trachomatis, te adenovirus, me ētahi atu, me te DNA ira tangata. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te Whakaora Tango Waikawa Karihi, te Whakaora Pure rānei (YDP315-R) nā Tiangen Biotech (Beijing) Co.,Ltd. Me mahi te tango kia rite tonu ki ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 140μL, ā, ko te rahinga wairewa i tūtohutia he 60μL.
Kōwhiringa 2.
Kete Whakamātautau Makro me te Iti mō te Ira/RNA Huakita (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Pūwero Waikawa Matū Aunoa Whakamātautau Makro me te Iti (HWTS-3006C, HWTS-3006B). Me mahi te tango kia rite ki ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 200μL, ā, ko te rahinga wairewa i tūtohutia he 80μL.
