Klebsiella Pneumoniae, Acinetobacter Baumannii me Pseudomonas Aeruginosa me ngā Ira Ātete Rongoā (KPC, NDM, OXA48 me IMP) Multiplex
Ingoa hua
Kete Kimi Maha o te HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii me Pseudomonas Aeruginosa me ngā Ira Ātete Rongoā (KPC, NDM, OXA48 me IMP) (PCR Fluorescence)
Tiwhikete
CE
Te mate urutā
Ko te Klebsiella pneumoniae he mate urutā haumanu noa, ā, ko tētahi o ngā huakita nui e mate ai ngā mate i roto i te tinana. Ina heke te ātete o te tinana, ka uru ngā huakita ki roto i ngā pukahukahu mai i te ara manawa, ka pā te mate ki ngā wāhanga maha o te tinana, ā, ko te whakamahinga wawe o ngā paturopi te mea nui ki te whakaora.[1].
Ko ngā pūkahukahu te wāhi tino kitea o te mate Acinetobacter baumannii, he mate nui tēnei mō te niumōnia i whiwhihia e te hōhipera (HAP), inā koa ko te niumōnia e pā ana ki te manawa (VAP). He maha ngā wā ka haere tahi me ētahi atu mate kitakita me te harore, me ngā āhuatanga o te nui o te mate me te nui o te mate.
Ko te Pseudomonas aeruginosa te bacilli gram-negative kore-whakapūkara tino kitea i roto i te mahi haumanu, ā, he pathogen whai wāhi nui mō te mate i riro mai i te hōhipera, me ngā āhuatanga o te noho ngāwari, te rerekētanga ngāwari me te ātete ki ngā rongoā maha.
Hongere
| Ingoa | PCR-Ranu 1 | PCR-Ranu 2 |
| Hongere FAM | Āpa | IMP |
| Hongere VIC/HEX | Mana Whakahaere ā-roto | KPC |
| Hongere CY5 | PA | NDM |
| Hongere ROX | KPN | OXA48 |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Huware |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Te motuhaketanga | a) E whakaatu ana te whakamātautau tauhohenga-whakawhiti kāore he tauhohenga-whakawhiti o tēnei kete ki ētahi atu mate manawa, pērā i te Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus me ngā tauira huware kāore he ūnga, me ētahi atu. b) Te kaha ārai-whakararuraru: Kōwhiria te mucin, te minocycline, te gentamicin, te clindamycin, te imipenem, te cefoperazone, te meropenem, te ciprofloxacin hydrochloride, te levofloxacin, te waikawa clavulanic, me te roxithromycin, me ētahi atu mō te whakamātautau whakararuraru, ā, e whakaatu ana ngā hua kāore ngā matū whakararuraru kua whakahuatia i runga ake nei e whakararuraru i te kitenga o ngā ira ātete Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa me te carbapenem KPC, NDM, OXA48 me IMP. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Otinga PCR Katoa
