Waenga Rawhiti Respiratory Syndrome Coronavirus Waikawa Nucleic

Whakaahuatanga Poto:

Ka whakamahia te kete mo te kitenga tohu o te MERS coronavirus waikawa nucleic i roto i te nasopharyngeal swabs me te Middle East Respiratory Syndrome (MERS) coronavirus.

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Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Kete Rapu Waikawa Nucleic(Fluorescent PCR)

Epidemiology

Middle East respiratory syndrome coronavirus (MERS-CoV), he β-coronavirus ka putamate manawa i roto i te tangata, i kitea tuatahi i roto i te mate 60-tau-tawhito Saudi Arabian manawanui tane i runga i te 24th Hōngongoi, 2012. Ko te whakaaturanga haumanu o MERS-ngaahi mate awhe i te āhua asymptomatic ranei tohu manawa romahā ngawari ki tino mate manawa whakapeka mate.

Hongere

FAM	MERS huaketo RNA
VIC(HEX)	Mana o roto

Tawhā Hangarau

Rokiroki	≤-18 ℃ I te pouri
Papa-ora	9 marama
Momo Tauira	Kohia hou nasopharyngeal swabs
CV	≤5.0%
Ct	≤38
LoD	1000 kape/mL
Te motuhake	Karekau he tauhohenga whakawhiti me nga coronaviruses tangata coronavirus tangata SARSr-CoV me etahi atu tukumate noa.
Nga taputapu e tika ana:	Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu

Rere Mahi

Kōwhiringa 1.

Reagent tangohanga Manakohia: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.

Kōwhiringa 2.

Reagent tangohanga Manakohia: Tohutono & Micro-Test General DNA/RNA Kit (HWTS-3017) me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

Tōmua: Waikawa Nucleic SARS-CoV-2

Panuku: SARS-CoV-2 IgM/IgG Antibody

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