Waikawa Matūriki Ira MTHFR
Ingoa hua
Kete Kimi Waikawa Matūriki Ira HWTS-GE004-MTHFR (ARMS-PCR)
Te mate urutā
He huaora wairewa-wai te waikawa folic, he mea nui ki ngā ara pākia o te tinana. I ngā tau tata nei, he maha ngā rangahau kua whakaū ko te whakarerekētanga o te ira whākōkī MTHFR e pāngia ai te tinana e te korenga o te waikawa folic, ā, ko te kino noa o te korenga o te waikawa folic i roto i ngā pakeke ka pāngia e te anemia megaloblastic, te kino o te endothelial vascular, me ētahi atu. Kāore e taea e te korenga o te waikawa folic i roto i ngā wāhine hāpu te whakatutuki i ngā hiahia o rātou anō me te tamaiti i roto i te pēpi, ka pāngia ai e ngā hapa ngongo neural, te anencephaly, te whānautanga mate, me te mate whānau. Ka pāngia ngā taumata folate toto e ngā momo āhua o te 5,10-methylenetetrahydrofolate reductase (MTHFR). Ko ngā whakarerekētanga 677C>T me te 1298A>C i roto i te ira MTHFR ka puta te hurihanga o te alanine ki te waikawa valine me te waikawa glutamic, ia, ka heke te mahi MTHFR, ā, ka heke te whakamahinga o te waikawa folic.
Hongere
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Mana whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Toto EDTA hou kua kohia |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Ngā Taputapu e Pā Ana: | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu SLAN ®-96P Ngā Pūnaha PCR Wā-Tūturu a QuantStudio™ 5 Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1
Ngā matū tango e taunakitia ana: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Kōwhiringa 2
Ngā matū tango e taunakitia ana: Kete Tango Ira Ira Toto (YDP348, JCXB20210062) nā Tiangen Biotech (Beijing) Co.,Ltd. Kete Tango Ira Toto (A1120) nā Promega.
