Ko te waikawa Mycobacteriosis waikawa me te riri rifampin
Ingoa Hua
HWST-RT074B-MycoAMosiosis waikawa Nukuna me te kiri kino o te tarukino a rifampicin (ka rewa te pihi)
Tiwihikete
CE
Manganga
MycobaCterium Kohuru, he rite ki te hinu ngongo, TB, ko te huakita pathogen e raru ana te mate kohi. I tenei wa, ko te rongoa anti-otaota tuatahi i whakamahia, ko te raau taero anti-otaota me te kanamycin, me etahi atu . Heoi, na te whakamahi i te kino o Ko nga raau anti-otaota me nga ahuatanga o te kohinga taiepa o te mate urupara, ka tipu te mate urupa Mycobactelis ki te aukati me te maimoatanga o te mate kohi.
Kua whakamahia e rifampicin i te maimoatanga o nga turoro o te mate pukupuku totokore mai i te mutunga o nga tau 1970, he nui te painga. Koinei te whiringa tuatahi ki te whakapoto i te Chepotherapy o nga turoro o te mate kohi mate pukupuku. Ko te riri o te rifampicin e tino hua ana e te kohinga o te ira rpob. Ahakoa ko nga raau anti-otaota hou kei te haere tonu, ka kaha tonu te whakamahi i te haumanu ki te rongoa o te mate pukupuku, me te ahuatanga o te tarukino anti-otaota. Ma te papu, kaore e tino patua te mate pukupuku mycobacterium i roto i nga turoro me te mate o te tarukino, ka kaha ake te mate o te mate.
Takiwā rerenga
| Takiwā rerenga | Tuhinga ka whai mai | Whakahauhau i te | Te urupare urupare b | Tauhohenga whana c |
| He whanau | Kairipoata: Whanau, Quencher: Kaore | rpob 507-514 | rpob 513-520 | 38kd me te IS6110 |
| Channel CY5 | Kairipoata: CY5, quencher: Kaore | rpob 520-527 | rpob 527-533 | / |
| Hex (Vic) Channel | Kairipoata: Hex (Vic), Quencher: Kaore | Te mana o roto | Te mana o roto | Te mana o roto |
Tohu hangarau
| Pākoro | ≤-18 ℃ i roto i te pouri |
| Te Haahi-Ora | 12 marama |
| Momo tauira | Sputum |
| CV | ≤5.0% |
| Koropū | MycobaCterium te huakita 50 o te huakita / ml Momo Wifampicin-Taumaha Momo: 2x103Pūkino / ML Mutant homozygous: 2x103Pūkino / ML |
| Whakawhāititanga | Ka kitea e ia te waikawa mycobacterosis mohoao me nga papaanga mo etahi atu ira tarukino penei i te katg 315g> C \ A inukia-15c> |
| Nga taputapu e tika ana: | Slan-96P Pūnaha PCR tūturu Biorad CFX96 Pūnaha Real-Time Pūnaha PaetahiWhakaahuaWhakaahua |
Rere mahi
Mena ka whakamahi i te macro & Micro-Rona Micro / RNA Kit (HWTS-3019-5, HWS-3019-48, HWS-3019-48, HWS-3019-96) Ko te tangohanga o te waikawa a roto (HWS-3006C, HWTS-3006B)) me te mate-a-ringa-whakamatautau ranei DNA / RNA Tīwae (HWS-3022-50) na Jiangsu Macro & Middo-Whakamatau Mid-O Tech-Tech CO., Ltd. Mo te tangohanga, Tāpiri 200μL te mana whakahaere, te mana whakahaere kino me te tauira Sputum Sputum. 10μL o te whakahaere o roto motuhake ki te mana whakahaere, te mana whakahaere kino me te tukatuka Sputum Sputum hei whakamatautau, me mahi tonu nga huarahi e whai ake nei E ai ki nga tohutohu tangohanga. Ko te kohinga tauira kua tangohia ko te 200μl, a ko te kohinga kohinga e taunakitia ana ko te 100μl.
