Te Ātete ki te Waikawa Nukleika me te Rifampicin i te Mycobacterium Tuberculosis
Ingoa hua
Kete Kimi Ātete ki te Waikawa me te Rifampicin HWTS-RT074B-Mycobacterium Tuberculosis (Pōro Rewa)
Tiwhikete
CE
Te mate urutā
Ko te Mycobacterium tuberculosis, ko te ingoa poto ko te Tubercle bacillus, TB, te huakita mate e hua ai te mate kohi. I tēnei wā, ko ngā rongoā tuatahi e whakamahia whānuitia ana ko te isoniazid, rifampicin me te hexambutol. Ko ngā rongoā tuarua e whakamahia whānuitia ana ko te fluoroquinolones, amikacin me te kanamycin. Ko ngā rongoā hou kua whanaketia ko te linezolid, bedaquiline me te delamani. Heoi, nā te whakamahinga hē o ngā rongoā me ngā āhuatanga o te hanganga o te pakitara pūtau o te mycobacterium tuberculosis, ka whakawhanakehia e te mycobacterium tuberculosis te ātete rongoā ki ngā rongoā, ka puta he wero nui ki te aukati me te rongoā i te mate kohi.
Kua whānuitia te whakamahinga o te Rifampicin hei rongoā i ngā tūroro mate kohi pūkahukahu mai i te mutunga o te tekau tau atu i 1970, ā, he nui te pānga. Koinei te whiringa tuatahi hei whakapoto i te chemotherapy mō ngā tūroro mate kohi pūkahukahu. Ko te ātete ki te Rifampicin te take nui o te whakarerekētanga o te ira rpoB. Ahakoa kei te puta tonu mai ngā rongoā hou hei ārai i te kohi, ā, kei te piki haere tonu te whai huatanga haumanu o ngā tūroro mate kohi pūkahukahu, kei te iti tonu ngā rongoā hei ārai i te kohi, ā, he teitei te whakamahinga rongoā koretake i roto i ngā rongoā haumanu. He mārama, kāore e taea te whakamate katoa i te Mycobacterium tuberculosis i roto i ngā tūroro mate kohi pūkahukahu i te wā tika, ā, ka mutu, ka rerekē te ātete rongoā i roto i te tinana o te tūroro, ka roa ake te mate, ka nui ake te mōrearea o te mate o te tūroro.
Hongere
| Hongere | Ngā Hongere me ngā Pūorooro | Pūwero Tauhohenga A | Pūwero Tauhohenga B | Pūwero Tauhohenga C |
| Hongere FAM | Kairīpoata: FAM, Quencher: Kāore | rpoB 507-514 | rpoB 513-520 | 38KD me IS6110 |
| Hongere CY5 | Kairīpoata: CY5, Quencher: Kāore | rpoB 520-527 | rpoB 527-533 | / |
| Hongere HEX (VIC) | Kairīpoata: HEX (VIC), Quencher: Kāore | Mana whakahaere ā-roto | Mana whakahaere ā-roto | Mana whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ I te pōuri |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Huware |
| CV | ≤5.0% |
| LoD | te mate pukupuku mycobacterium 50 huakita/mL momo mohoao ātete-rifampicin: 2x103huakita/mL ira whakarerekētanga takatāpui: 2x103huakita/mL |
| Te motuhaketanga | Ka kitea e ia te mycobacterium tuberculosis momo-mohoao me ngā wāhi whakarerekētanga o ētahi atu ira ātete rongoā pērā i te katG 315G>C\A, InhA-15C> T, kāore ngā hua whakamātautau e whakaatu ana i te ātete ki te rifampicin, ko te tikanga kāore he tauhohenga whakawhiti. |
| Ngā Taputapu e Pā Ana: | Ngā Pūnaha PCR Wā-Tūturu SLAN-96P Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu LightCycler480® |
Rere Mahi
Ki te whakamahia te Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) te Macro & Micro-Test Viral DNA/RNA Column rānei (HWTS-3022-50) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mō te tango, tāpirihia te 200μL o te tauira Mana Pai, te mana kino me te tauira huware tukatuka hei whakamātautau i roto i te raupapa, ka tāpirihia te 10μL o te tauira mana ā-roto ki roto i te tauira Mana Pai, te mana kino me te tauira huware tukatuka hei whakamātautau, ā, me tino mahi ngā mahi e whai ake nei kia rite ki ngā tohutohu tango. Ko te rahinga tauira i tangohia he 200μL, ā, ko te rahinga wairewa kua tūtohutia he 100μL.
