Mai i te marama o Mei 2022, kua pūrongohia ngā kēhi MPOX i roto i ngā whenua maha o te ao kāore i te horapa te mate urutā me te tuku i te mate ki te hapori.
I te 26 o Ākuhata, i whakarewahia e te Whakahaere Hauora o te Ao (WHO) he kaupapa ā-aoTe Whakarite Rautaki me te Mahere Uruparehei aukati i ngā pakarutanga o te tuku i te mate urutā MPOX mai i te tangata ki te tangata mā roto i ngā mahi ā-ao, ā-rohe, me te ā-motu kua whakaritea. Whai muri i te whakapuakitanga o tētahi āhuatanga ohorere hauora tūmatanui e āwangawangatia ana e te Kaiwhakahaere Matua o te WHO i te 14 o Ākuhata.
Me mōhio he rerekē te pakarutanga o te MPOX i tēnei wā i tērā i te tau 2022, i horapa nui atu i waenga i ngā tāne e moe tāne ana, ā, ko te reiti mate o te hunga i pangia he iti iho i te 1%.
Ko te momo "Clade Ib" e horapa ana i ēnei rā, he momo o te Clade I, he nui ake te mate. I tīmata te horapa o tēnei momo hou ki te DRC i te marama o Hepetema kua taha ake nei, i te tīmatanga i waenga i ngā kaimahi ira tangata, ā, kua horapa inaianei ki ētahi atu rōpū, me ngā tamariki e tino pāngia ana.
I roto i tētahi pūrongo a te Africa CDC i te marama kua taha ake nei, i kī te pūrongo a Africa CDC kua kitea ngā pakarutanga o te mpox i roto i ngā whenua 10 o Āwherika i tēnei tau, tae atu ki te Democratic Republic of Congo (DRC), nāna nei i pūrongo te 96.3% o ngā kēhi katoa i Āwherika i tēnei tau, me te 97% o ngā matenga. He mea tika kia mōhiotia tata ki te 70% o ngā kēhi i roto i te Democratic Republic of Congo he tamariki kei raro iho i te 15 tau te pakeke, ā, ko tēnei rōpū te 85% o ngā matenga i roto i te whenua.
He mate zoonotic te Mpox nā te huaketo mpox, ā, ko te wā whakatipuranga he 5 ki te 21 ngā rā, arā, ko te nuinga he 6 ki te 13 ngā rā. Ka pāngia te tangata pangia e te kirika, te ānini me te pupuhi o ngā kōpū lymph, ā, ka puta he ponana ki te mata me ētahi atu wāhanga o te tinana, ka huri haere hei pustule, ā, ka roa pea mō te wiki pea i mua i te puta o te hakihaki. Ka horapa te mate mai i te tīmatanga o ngā tohu tae noa ki te ngaromanga noa o ngā hakihaki.
Kei te whakarato a Macro & Micro-Test i ngā whakamātautau tere, ngā kete ngota ngota me ngā otinga raupapatanga mō te kimi huaketo mpox, hei āwhina i te tātaritanga huaketo mpox i te wā tika, te tirotiro i tōna takenga mai, te whakapapa, te tuku me ngā rerekētanga ira:
Ārai Mate Huaketo MonkeypoxKete Kimi (Te Immunochromatography)
He māmā te tango tauira (he wai ponana/tauira korokoro) ā, ka tere te puta o ngā hua i roto i te 10-15 meneti;
Teitei o te aro me te LoD o te 20pg/mL e kapi ana i te Clade I me te II;
He tino motuhake, kāore he tauhohenga whakawhiti ki te huaketo rewharewha, te huaketo varicella zoster, te huaketo rubella, te huaketo herpes simplex, me ētahi atu.
OPA o te 96.4% ki te whakaritea ki ngā NAAT;
He whānuitia te whakamahinga pērā i ngā tikanga tāke, ngā CDC, ngā whare rongoā, ngā wāhi hauora, ngā hōhipera, i te kāinga rānei.
Kete Kimi Paturopi IgM/IgG mō te Huaketo Monkeypox(Immunochromatography)
He ngāwari te whakamahi i ngā taputapu, ā, ka tere te hua i roto i te 10 meneti;
Teitei o te aro me te motuhake e hipoki ana i te Clade I me te II;
Ka tautuhi i te IgM me te IgG hei whakatau i ngā wāhanga o te mate mpox;
He whānui te whakamahinga pērā i ngā tikanga, ngā CDC, ngā whare rongoā, ngā wāhi hauora, ngā hōhipera, i te kāinga rānei;
He mea tika mō te tirotiro nui i te hunga e whakapaetia ana he mate mpox.
Kete Kimi Waikawa Matū mo te Huaketo Monkeypox (Whakanuia te Whakamātautau Enzymatic Isothermal)
Te tairongo teitei me te LoD o te 200 Tārua/mL me te IC, he ōrite ki te PCR whakakorikori;
Ngāwari te whakahaere: I tāpirihia te tauira kua tangohia ki te ngongo matū kua whakakīia kia tika ai te whakanui i runga i te tono mā te whakahohe i ngā kōwae motuhake o te Pūnaha Whakakaha Ngāwari;
He tino motuhake, kāore he tauhohenga whakawhiti ki te huaketo rewharewha, te huaketo vaccinia, te huaketo rewharewha kau, te huaketo rewharewha kiore, te huaketo herpes simplex, te huaketo varicella-zoster, me te ira tangata, me ētahi atu;
He māmā te tango tauira (he wai ponana/he ūkui o te waha) ā, ka tere te puta o te hua pai i roto i te 5 meneti;
He tino pai te mahi haumanu e kapi ana i te Clade I me te II me te PPA o te 100%, te NPA o te 100%, te OPA o te 100% me te Kappa Value o te 1.000 ki te whakatauritea ki te kete Fluorescence PCR;
Mā te putanga lyophilized e hiahia ana kia kawe me te rokiroki i te pāmahana rūma anake ka taea te uru atu ki ngā rohe katoa;
Ngā āhuatanga ngāwari i roto i ngā whare hauora, i te pokapū hauora, me te Easy Amp mō te kimi i runga i te tono;
Kete Kimi Waikawa Matū mo te Huaketo Monkeypox (PCR Fluorescence)
Ira takirua kua arotahihia me te aro nui me te LoD o te 200 kape/mL;
Te tango tauira ngāwari o te wai ponana, te ūkui korokoro me te serum;
He tino motuhake, kāore he tauhohenga whakawhiti ki te huaketo rewharewha, te huaketo vaccinia, te huaketo rewharewha kau, te huaketo rewharewha kiore, te huaketo herpes simplex, te huaketo varicella-zoster, me te ira tangata, me ētahi atu;
Ngāwari te whakahaere: ka tere te pakaru o te tauira mā te matū tuku tauira hei tāpiri atu ki te ngongo tauhohenga;
Te kitenga tere: ka puta te hua i roto i te 40 meneti;
Ka whakapūmautia te tika e te mana whakahaere ā-roto e tirotiro ana i te tukanga kimi katoa;
He tino pai te mahi haumanu e kapi ana i te Clade I me te II me te PPA o te 100%, te NPA o te 99.40%, te OPA o te 99.64% me te Uara Kappa o te 0.9923 ki te whakatauritea ki te raupapatanga;
Mā te putanga lyophilized e hiahia ana kia kawe me te rokiroki i te pāmahana rūma anake ka taea te uru atu ki ngā rohe katoa;
Hototahi ki ngā Pūnaha PCR Fluorescence matua;
Ngā horopaki ngāwari mō ngā hōhipera, ngā CDC me ngā taiwhanga;
Kete Kimi Waikawa Matū mo te Huaketo Orthopox Momo Ao/Huaketo Maki (PCR Fluorescence)
Kapinga katoa: ka whakamātautau i ngā huaketo orthopox e 4 ka pangia te tangata me te mpox whānui (tae atu ki te Clade I&II) i roto i te whakamātautau kotahi hei karo i te ngaro o te kitenga;
Te tairongo nui me te LoD o te 200 kape/mL;
He tino motuhake, kāore he tauhohenga whakawhiti ki ētahi atu mate e puta ai ngā ponana pēnei i te huaketo herpes simplex, te huaketo varicella-zoster, me te ira tangata, me ētahi atu;
Ngāwari te whakahaere: ka tere te pakaru o te tauira mā te matū tuku tauira hei tāpiri atu ki te pūwero tauhohenga ngongo kotahi;
Te kimi tere: te whakanui tere me te hua i roto i te 40 meneti;
Ka whakapūmautia te tika e te mana whakahaere ā-roto e tirotiro ana i te tukanga kimi katoa;
Hototahi ki ngā Pūnaha PCR Fluorescence matua;
Ngā horopaki ngāwari mō ngā hōhipera, ngā CDC me ngā taiwhanga;
RewharewhaViru Tte ypingNucleicAwaikawaDte āraiKia (F(PCR kanapa)
Ka tautuhi i te Clade I me te Clade II i te wā kotahi, he mea nui tēnei mō te mārama ki ngā āhuatanga mate urutā o te huaketo, te whai i tōna horapa, me te whakatakoto i ngā mahi ārai me te whakahaere kua arotahihia.
Te tairongo nui me te LoD o te 200 kape/mL;
Te tango tauira ngāwari o te wai ponana, te ūkui waha me te toto;
He tino motuhake, kāore he tauhohenga whakawhiti i waenga i te Clade I me te II, ētahi atu mate pukupuku e hua ai ngā ponana pēnei i te huaketo herpes simplex, te huaketo varicella-zoster, me te ira tangata, me ētahi atu;
Ngāwari te whakahaere: ka tere te pakaru o te tauira mā te matū tuku tauira hei tāpiri atu ki te pūwero tauhohenga ngongo kotahi;
Te kitenga tere: ka puta te hua i roto i te 40 meneti;
Ka whakapūmautia te tika e te mana whakahaere ā-roto e tirotiro ana i te tukanga kimi katoa;
Mā te putanga lyophilized e hiahia ana kia kawe me te rokiroki i te pāmahana rūma anake ka taea te uru atu ki ngā rohe katoa;
Hototahi ki ngā Pūnaha PCR Fluorescence matua;
Ngā horopaki ngāwari mō ngā hōhipera, ngā CDC me ngā taiwhanga;
Te Huaketo Makimaki o te Ira Katoa o te AoTe kimiKete (NGS maha-PCR)
Ka taea e te Kete Kimi Ira Katoa o te Huaketo Monkeypox hou te hanga mā te Macro & Micro-Test mō ngā āhuatanga rerekē, me te whakakotahitanga o te ONT nanopore sequencing, te whiwhi i te raupapatanga ira katoa MPXV me te kapinga o te 98% i roto i ngā haora 8.
Ngāwari ki te whakahaere: he hangarau whakanui kotahi-taahiraa kua patenttia, ka taea te whiwhi i te raupapa ira katoa o te huaketo mpox mā te whakanui kotahi-a tawhio noa;
He tairongo, he tika hoki: ka kitea ngā tauira iti ki te 32CT, ā, ka taea e te raupapatanga nanopore amplicon 600bp te whakatutuki i te huihuinga ira kounga teitei ake;
Tino tere: Ka taea e te ONT te whakaoti i te huihuinga ira i roto i ngā haora 6-8;
Hototahi whānui: me ONT, Qi Carbon, SALUS Pro, lllumina, MGI me ētahi atu 2 matuandme te 3rdngā pūwhakarārangi whakatupuranga.
Tino tairongoTe Ira Katoa o te Huaketo MakiTe kimiKete-Illumina/MGI(NGS maha-PCR)
Mō te nui o ngā mea e rua o nāianeindmō ngā raupapatanga whakatupuranga puta noa i te ao, kua whakawhanakehia hoki e Macro & Micro-Test ngā kete tino tairongo e urutau ana ki ngā raupapatanga matua hei whakatutuki i te raupapatanga ira huaketo tauira iti-kukū;
Whakareitanga whai hua: 1448 ngā takirua o te hoahoa paninga tino matotoru 200bp mō te whai huatanga whakareitanga teitei me te hipoki ōrite;
Ngāwari te whakahaere: Ka taea te tiki i te whare pukapuka huaketo Mpox llumina/MGI mā te whakanui rua-wāhanga i roto i ngā hāora e 4, kia kore ai e uaua te hanga whare pukapuka me ngā utu matū;
Teitei o te aro: ka kitea ngā tauira iti ki te 35CT, ka ārai i ngā hua kino teka i puta mai i te pakaru o ngā kongakonga, i te iti rānei o te maha o ngā tārua;
He whānui te hototahitanga ki te ao matua 2ndngā pūtāhui whakatupuranga pēnei i a lllumina, Salus Pro, MGI rānei;Tae noa mai ki tēnei wā, neke atu i te 400 ngā kēhi haumanu kua oti.
Wā tuku: 28 o Ākuhata-2024