Clostridium dificile toxin a / b ira (c.diff)
Ingoa Hua
HWST-OT031A Ko te kete werohanga waikawa not031
Tiwihikete
CE
Manganga
Ko te ClOstridium Dicfilica (CD), he karamu-cram-pai anaerobic smoutnium smostridium dficalical, ko tetahi o nga pathogens matua i mate aisocolial mate. Ma te haumanu, neke atu i te 15% 25% o te mate antimicrobial e pa ana, 50% 75% o te kohinga antimicrobial me te 95% o te kainoni pseudomemrane na te clostridium Dilficile Idfious Diplificile (CDI). Ko te ClOstridium Dicficile he pathogen haangai, tae atu ki nga riipene toxigenic me nga uaua kore-toxigenic.
Takiwā rerenga
| Whānau | tcdatangata |
| Rox | tcdbtangata |
| Vic / Hex | Te mana o roto |
Tohu hangarau
| Pākoro | ≤-18 ℃ |
| Te Haahi-Ora | 12 marama |
| Momo tauira | manga |
| Tt | ≤38 |
| CV | ≤5.0% |
| Koropū | 200cfu / ml |
| Whakawhāititanga | Whakamahia tenei kete ki te kite i etahi atu pathichina coli, saphylococcus aureus, shigella, satmonium, mirovirus dietszar DNA, TE He kino nga hua. |
| Nga taputapu e tika ana | Te tono Biosystems 7500 nga punaha PCR-tūturu Te tono Biosystems 7500 nga punaha PCR-tino-tino tere Āneni®5 pūnaha procr pocr tūturu Slan-96p Real-96P tūturu (Hongshi Medical Hangarau Hangarau Co., Ltd.) Tairapa®480 Pūnaha PCR Real LINEGENE 9600 PLUL DATER TRACTRANGA PCR (FQD-96AKāore,HangzhouHangarau Bioer) Ma-6000 Motuhake Thermal Thermal-Time (Suzhou Molahy Co., Ltd.) Biorad CFX96 Pūnaha Real-Time Biorad CFX Opus 96 Pūnaha Real-Time |
Rere mahi
Kōwhiringa 1.
Tāpirihia ki te 180μl o te besozyme Buffer ki te tiihi (waimeha i te lysozyme ki te 20mg / ml me te riihi kia pai ake ai te 30 meneti.Take. me te tāpiri180μL o te mana whakahaere me te whakahaere kino i te raupapa. Tāpiri10μL o te mana whakahaere ki te tauira ka whakamatautauria, ki te whakahaere kino, ki te whakamahi i te tangohanga waikawa a te Tiangen Biotech (Beijing) Co., Ltd. Tena koa te whai i nga tohutohu hei whakamahi mo nga waahanga motuhake. Whakamahia te DNASE / RNASE FREE H2O mo te Eltive, a ko te rōrahi envined e taunakitia ana ko te 100μl.
Kōwhiringa 2.
Tangohia te 1.5ml o te Rnase / DNASE-KOREUTU TUPE, me te taapiri i te 200μL o te mana whakahaere me te whakahaere kino i te raupapa. Tāpiri10μμ o te mana whakahaere ki te tauira kia whakamatautauria, te mana whakahaere, me te whakahaere kino, me te whakamahi i te Macro & RNA Kit (HWS-3004-48, HWS-3004-48, HWS-3004-48, HWS-3004-48 96) me te macro & Te Micro-whakamatautau aunoa i te tangohanga waikawa Nuku (HWS-3006). Me kawe te tangohanga i roto i te kaha ki te tohutohu mo te whakamahinga, me te kohinga tino pai ko te 80μl.
