Clostridium difficile toxin A/B ira（C.diff）
ingoa hua
HWTS-OT031A Kete Rapu Waikawa Nucleic mo te Clostridium difficile toxin A/B ira (C.diff) (Fluorescence PCR)
Tiwhikete
CE
Epidemiology
Ko Clostridium difficile (CD), he karamu pai anaerobic sporogenic Clostridium difficile, ko tetahi o nga tukumate matua e mate ai nga whekau nosocomial. Haumanu, mo te 15% ~ 25% o te mate pukupuku e pa ana ki te antimicrobial, 50% ~ 75% o te colitis e pa ana ki te antimicrobial me te 95% ~ 100% o te enteritis pseudomembranous na te mate Clostridium difficile (CDI). Ko te Clostridium difficile he tukumate here, tae atu ki nga riaka toxigenic me nga riaka kore-toxigenic.
Hongere
| FAM | tcdAira |
| ROX | tcdBira |
| VIC/HEX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | kurupae |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Tauwhāiti | whakamahia tenei kete ki te kite i etahi atu tukumate intestinal penei i te Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic riaka, Adenovirus, rotavirus, norovirus, rewharewha A huaketo, rewharewha B huaketo me nga hua ira tangata kino katoa. |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu(FQD-96A，HangzhouHangarau Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Taapirihia te 180μL o te lysozyme buffer ki te waipara (whakarihia te lysozyme ki te 20mg/mL me te lysozyme diluent), te paipa paipa ki te uru pai, ka tukatuka i te 37°C mo te neke atu i te 30 meneti. Tangohia te 1.5mL o te ngongo centrifuge RNase/DNase-kore, ka tapiri180μL o te mana pai me te mana kino i roto i te raupapa. Tāpiri10μL o te mana o roto ki te tauira kia whakamatauria, te whakahaere pai, me te whakahaere kino i roto i te raupapa, me te whakamahi i te Nucleic Acid Extraction or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Whakamahia te DNase/RNase kore utu H2O mo te elution, a ko te 100μL te rōrahi elution e taunaki ana.
Kōwhiringa 2.
Tangohia 1.5mL o RNase/DNase-free centrifuge ngongo, ka tāpiri 200μL o te mana pai me te mana kino i roto i te raupapa. Tāpiri10μL o te mana o roto ki te tauira kia whakamatauria, te mana pai, me te mana kino i roto i te raupapa, me te whakamahi i te Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor-30 (HWTS-3004-32). Ko te tangohanga me tino rite ki nga tohutohu mo te whakamahi, a ko te 80μL te rahinga elution e taunaki ana.
