Ira A/B o te paitini Clostridium difficile（C.diff）
Ingoa hua
Kete Kimi Waikawa Karihi HWTS-OT031A mō te ira paitini Clostridium difficile A/B (C.diff) (Fluorescence PCR)
Tiwhikete
CE
Te mate urutā
Ko te Clostridium difficile (CD), he Clostridium difficile sporogenic anaerobic gram-positive, tētahi o ngā mate pukupuku matua e puta ai ngā mate puku i roto i te whare. I roto i te rangahau haumanu, tata ki te 15%~25% o te mate korere e pā ana ki te antimicrobial, 50%~75% o te colitis e pā ana ki te antimicrobial me te 95%~100% o te enteritis pseudomembranous e puta mai ana i te mate Clostridium difficile (CDI). He mate pukupuku ā-tinana te Clostridium difficile, tae atu ki ngā momo toxigenic me ngā momo kore-toxigenic.
Hongere
| FAM | tcdAira |
| ROX | tcdBira |
| VIC/HEX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | tūru |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Te motuhaketanga | Whakamahia tēnei kete hei kimi i ētahi atu mate puku pērā i te Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus me te DNA ira tangata, ā, he kino katoa ngā hua. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 PlusFQD-96A，HangzhouHangarau Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Tāpirihia he 180μL o te pūwero lysozyme ki te wairewa (whakaranua te lysozyme ki te 20mg/mL me te wairewa lysozyme), tāpirihia he pipette kia pai te whakaranu, ā, tukatukahia i te 37°C mō te neke atu i te 30 meneti. Tangohia he 1.5mL o te ngongo centrifuge kore-RNase/DNase, ka tāpirihia.180μL o te mana whakahaere pai me te mana whakahaere kino i te raupapatanga. Tāpirihia10μL o te mana whakahaere ā-roto ki te tauira hei whakamātautau, te mana whakahaere pai, me te mana whakahaere kino i te raupapatanga, ā, whakamahia te Nucleic Acid Extraction or Purification Reagent (YDP302) nā Tiangen Biotech (Beijing) Co., Ltd. mō te tangohanga DNA tauira i muri mai, ā, me whai pūmau i ngā tohutohu whakamahi mō ngā mahi motuhake. Whakamahia te DNase/RNase free H2O mō te whakaputanga, ā, ko te rōrahi whakaputanga e taunakitia ana ko 100μL.
Kōwhiringa 2.
Tangohia te 1.5mL o te ngongo whakapūtātanga kore-RNase/DNase, ka tāpirihia te 200μL o te mana whakahaere pai me te mana whakahaere kino i roto i te raupapa. Tāpirihia10μL o te mana whakahaere ā-roto ki te tauira hei whakamātautau, te mana whakahaere pai, me te mana whakahaere kino i te raupapatanga, ā, whakamahia te Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Me whakahaere te tangohanga i runga i te tino rite ki ngā tohutohu whakamahi, ā, ko te rōrahi wairewa kua tūtohutia he 80μL.
