Orientia tsutsugamushi Waikawa Nucleic
ingoa hua
HWTS-OT002-Orientia tsutsugamushi Kete Rapu Waikawa Nucleic(Fluorescence PCR)
Epidemiology
Ko te Scrub typhus he mate febrile whakapeka na te mate Orientia tsutsugamushi (Ot). Ko Orientia scrub typhus he moroiti parasitic intracellular obligate Gram-negative. Ko Orientia scrub typhus no te puninga Orientia no te raupapa Rickettsiales, te whanau Rickettsiaceae, me te puninga Orientia. Ko te nuinga o nga wa ka tukuna te tipu tipu ma te ngau o nga torongū chigger e mau ana i nga mate tukumate. Kei te haumanu te ahua o te kirika nui ohorere, te eschar, te lymphadenopathy, te hepatosplenomegaly, me te leukopenia toto peripheral, me etahi atu I roto i nga keehi kino, ka taea e ia te mate pukupuku, te ate me te whatukuhu, te ngoikore o te tinana maha, tae atu ki te mate.
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | tauira serum |
| CV | ≤5.0% |
| LoD | 500 kape/μL |
| Nga taputapu e tika ana | E tika ana ki te reagent rapunga momo I: Hangaia nga Pūnaha Koiora 7500 Pūnaha PCR Wā-tūturu, QuantStudio®5 Pūnaha PCR Wā-tūturu, SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer), MA-6000 Waa-Tuturu Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.), BioRad CFX96 Pūnaha PCR Wā-tūturu, BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu. E tika ana ki te reagent rapunga momo II: EudemonaTMAIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Rere Mahi
Tonotono & Micro-Test General DNA / RNA Kit (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 100μL.
