Orientia tsutsugamushi
ingoa hua
HWTS-OT002B Orientia tsutsugamushiKete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te Scrub typhus he mate febrile whakapeka na te mate Orientia tsutsugamushi (Ot). Ko Orientia scrub typhus he moroiti parasitic intracellular obligate Gram-negative. Ko Orientia scrub typhus no te puninga Orientia no te raupapa Rickettsiales, te whanau Rickettsiaceae, me te puninga Orientia. Ko te nuinga o nga wa ka tukuna te tipu tipu ma te ngau o nga torongū chigger e mau ana i nga mate tukumate. Kei te haumanu te ahua o te kirika nui ohorere, te eschar, te lymphadenopathy, te hepatosplenomegaly, me te leukopenia toto peripheral, me etahi atu I roto i nga keehi kino, ka taea e ia te mate pukupuku, te ate me te whatukuhu, te ngoikore o te tinana maha, tae atu ki te mate.
Hongere
| FAM | Orientia tsutsugamushi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | serum hou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kape/μL |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu RainaGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu, BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent tangohanga e manakohia ana: Tonotono me te Whakamatau MokoitiWhānuiKete DNA/RNA (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te rahinga tauira kua tangohia he 200µL, ko te rōrahi elution e taunaki ana ko100µL.
