Orientia tsutsugamushi
Ingoa hua
HWTS-OT002B Orientia tsutsugamushiKete Kimi Waikawa Karihi (PCR Whakapūngao)
Te mate urutā
He mate kirika nui te Scrub typhus i puta mai i te mate Orientia tsutsugamushi (Ot). He moroiti pirinoa ā-roto Gram-negative te Orientia scrub typhus. Nō te puninga Orientia i roto i te raupapa Rickettsiales, te whānau Rickettsiaceae, me te puninga Orientia te Orientia scrub typhus. Ko te nuinga o te wā ka horapa te Scrub typhus mā roto i ngā ngau a ngā torongū chigger e mau ana i ngā pathogens. Ko tōna āhua haumanu he kirika nui ohorere, he eschar, he lymphadenopathy, hepatosplenomegaly, me te leukopenia toto ā-taha, me ētahi atu. I ngā take kino, ka taea e ia te mate meningitis, te ngoikore o te ate me te whatukuhu, te ngoikore o te maha o ngā okana, tae atu ki te mate.
Hongere
| FAM | Orientia tsutsugamushi |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | serum hou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/μL |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Pūnaha PCR Wā-Tūturu 480 Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96, Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Te Whakamātautau-Makro me te Whakamātautau-ItiWhānuiKete DNA/RNA (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tangohanga kia rite ki ngā tohutohu mō te whakamahinga o tēnei matū tangohanga. Ko te rahinga tauira i tangohia he 200µL, ā, ko te rahinga wairewa i tūtohutia ko100µL.
