Whakakotahitia nga Tukino Respiratory
ingoa hua
HWTS-RT183-Kete Tirohanga Whakakotahitanga Whakahaehae Whakahaehae(Fluorescence PCR)
Epidemiology
Ko te Corona Virus Disease 2019, e kiia ana ko 'COVID-19', e pa ana ki te pneumonia na te mate SARS-CoV-2. Ko te SARS-CoV-2 he coronavirus no te puninga β. Ko te COVID-19 he mate hopuhopu rewharewha whakapeka, a, ko te nuinga o te taupori he whakaraerae. I tenei wa, ko te nuinga o nga turoro e pangia ana e te 2019-nCoV te take o te mate, a ko nga tangata kua pangia asymptomatic ka noho ano te take o te mate. I runga i nga rangahau mate urutaru o naianei, ko te wa whakamomori he 1-14 ra, ko te nuinga 3-7 ra. Ko te kirikaa, te maremare me te ngenge nga tohu nui. Ko etahi o nga turoro i puta nga tohu penei i te ihu ihu, te ihu rere, te korokoro, te myalgia me te korere, me era atu. Ko te rewharewha, e mohiotia ana ko 'rewharewha', he mate hopuhopu whakapeka na te huaketo rewharewha. He tino hopuhopu. Ko te nuinga o te wa ka tukuna ma te maremare me te tihe. Ko te tikanga ka pakaru i te puna me te takurua. Kua wehea nga mate rewharewha ki te rewharewha A (IFV A), rewharewha B (IFV B), me te rewharewha C (IFV C) e toru nga momo, no te huaketo piri katoa, ka puta te mate o te tangata mo te rewharewha A me te rewharewha B, he rewharewha kotahi, he wheori RNA kua wehea. Ko te huaketo rewharewha A he mate manawa whakapeka, tae atu ki te H1N1, H3N2 me etahi atu momo iti, e kaha ana ki te whakarereke me te pakarutanga puta noa i te ao. E tohu ana te 'Shift' ki te rerekeetanga o te rewharewha A wheori, ka puta mai he 'momo iti' huaketo hou. Kua wehea nga wheori rewharewha B ki nga whakapapa e rua, ko Yamagata me Wikitoria Rewharewha B ko te rewharewha B anake he paheketanga antigenic, a ka karo i te tirotiro me te whakakorenga a te tangata na roto i te whakarereketanga. Heoi, he puhoi ake te tere kunenga o te rewharewha B i te rewharewha A te tangata. Ka taea hoki e te rewharewha B te mate manawa o te tangata, ka arahi ki nga mate uruta.
Respiratory syncytial virus (RSV) he huaketo RNA, no te whanau paramyxoviridae. Ka tukuna mai e nga pata o te hau me te whakapiri tata, ko te mate matua o te mate rewharewha o raro o nga kohungahunga. Ko nga kohungahunga kua pangia e te RSV ka tupu pea te bronchiolitis kino me te pneumonia, e pa ana ki te mate huango i roto i nga tamariki. He kino nga tohu o nga kohungahunga, tae atu ki te kirikaa nui, te rhinitis, te pharyngitis me te laryngitis, katahi ka bronchiolitis me te pneumonia. Ko etahi o nga tamariki e mate ana ka uaua ki te otitis media, pleurisy me te myocarditis, etc.Ko te mate o te rewharewha o runga te tohu matua o te mate i roto i nga pakeke me nga tamariki pakeke.
Tawhā Hangarau
| Rokiroki | -18℃ I te pouri |
| Papa-ora | 9 marama |
| Momo Tauira | Te paninga oropharyngeal; Puka nasopharyngeal |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 kape/μL |
| Tauwhāiti | Ko nga hua o te mahi whakawhiti e whakaatu ana kaore he tauhohenga whakawhiti i waenga i te kete me te cytomegalovirus, herpes simplex virus momo 1, varicella zoster huaketo, Epstein-Barr huaketo, adenovirus, metapneumovirus tangata, rhinovirus, parainfluenza momo huaketo I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma perpneumonia, Borcoplasma pnemoniatus, Chlamydia pneumoniae, Chlamdettac spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, riaka whakangoikore o te Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp., Pseudomonas aeruginosaure, Staphylococcus aeropumonius, Staphylococcus aruginosaure epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus Ptismococcus, Aspergillus Ptismollusgatus carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q kirika me te waikawa nucleic genomic tangata. |
| Nga taputapu e tika ana | Biosystems Whakamahinga 7500 Pūnaha PCR Wā-tūturu, Pūnaha-koiora kua whakamahia 7500 Pūnaha PCR Wa-Tuturu Tere, QuantStudio®5 Pūnaha PCR Wā-tūturu, SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Pūnaha PCR Wā-Tuturu, LineGene 9600 Plus Pūnaha Tirohanga PCR Waa-Tuturu (FQD-96A, Hangarau Hangarau Hangzhou Bioer), MA-6000 Waa-Tuturu Ine Hukarere Ngaariki (Suzhou Molarray Co., Ltd.), BioRad CFX96 Pūnaha PCR Waa-Tuturu, BioRad CFX Opus Opus . |
Rere Mahi
Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), me te Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8 ka taea te whakamahi me Euch)TM AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ko 200μL te rōrahi tauira tangohia me te rōrahi elution tūtohu ko 150μL.
