SARS-CoV-2/rewharewha A/rewharewha B
Ingoa hua
Kete Kimi Waikawa Matū HWTS-RT148-SARS-CoV-2/rewharewha A/rewharewha B (PCR Whakapūmau)
Hongere
| Ingoa Hongere | PCR-Ranu 1 | PCR-Ranu 2 |
| Hongere FAM | Ira ORF1ab | IVA |
| Hongere VIC/HEX | Mana whakahaere ā-roto | Mana whakahaere ā-roto |
| Hongere CY5 | Ira N | / |
| Hongere ROX | Ira E | IVB |
Ngā Tawhā Hangarau
| Rokiroki | -18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | ngā ūkui ihu me ngā ūkui oro |
| Whāinga | Ngā ūnga e toru o te SARS-CoV-2 (ngā ira Orf1ab, N me E)/rewharewha A/rewharewha B |
| Ct | ≤38 |
| CV | ≤10.0% |
| LoD | SARS-CoV-2：300 Ngā Tārua/mL huaketo rewharewha A: 500 Tārua/mL huaketo rewharewha B: 500 Tārua/mL |
| Te motuhaketanga | a) I whakaatuhia e ngā hua o te whakamātautau whakawhiti he hototahi te kete ki te mate korona tangata SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, te huaketo syncytial manawa A me B, te huaketo parainfluenza 1, 2 me 3, te rhinovirusA, B me C, te adenovirus 1, 2, 3, 4, 5, 7 me 55, te metapneumovirus tangata, te enterovirus A, B, C me D, te huaketo pūkahukahu cytoplasmic tangata, te huaketo EB, te huaketo rewharewha Te cytomegalovirus tangata, te rotavirus, te norovirus, te huaketo mumps, te huaketo varicella zoster, te Mycoplasma pneumoniae, te Chlamydia pneumoniae, te Legionella, te pertussis, te Haemophilus influenzae, te Staphylococcus aureus, te Streptococcus pneumoniae, te Streptococcus pyogenes, te Klebsiella pneumoniae, te Mycobacterium tuberculosis, te Aspergillus fumigatus, te Candida albicans, Candida glabrata Kāore he tauhohenga whakawhiti i waenganui i a Pneumocystis yersini me Cryptococcus neoformans. b) Āheinga ārai pokanoa: tīpakohia te mucin (60mg/mL), 10% (V/ V) toto tangata, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), konutai pūhaumāota (kei roto he matū tiaki) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) me te tobramycin (0.6mg/mL). I whakaatuhia e ngā hua kāore he urupare pokanoa o ngā matū whakararuraru i ngā kukū o runga ake nei ki ngā hua o te kitenga o ngā pathogens. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 SLAN ®Ngā Pūnaha PCR Wā-Tūturu -96P Ngā Pūnaha PCR Wā-Tūturu a QuantStudio™ 5 LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Otinga PCR Katoa
