Te Huaketo Ebola o Zaire
Ingoa hua
Kete Kimi Waikawa Matū HWTS-FE008 mō te Huaketo Ebola Zaire (PCR Whakapūngao)
Te mate urutā
Nō Filoviridae te huaketo Ebola, he huaketo RNA kotahi-aho kore-wāhanga, he aho kino. He miro roa ngā huaketo, ko te roa toharite o te virion he 1000nm, ā, ko te whānui he 100nm pea. Ko te ira o te huaketo Ebola he RNA aho kino kore-wāhanga, he 18.9kb te rahi, e whakamārama ana i ngā pūmua hanganga e 7 me te pūmua kore-hanganga e 1. Ka taea te wehewehe i te huaketo Ebola ki ngā momo pēnei i a Zaire, Sudan, Bundibugyo, Tai Forest me Reston. I roto i ēnei, kua pūrongohia ko te momo Zaire me te momo Sudan te take o te mate o te tini tāngata. Ko te EHF (Ebola Hemorrhagic Fever) he mate pāwera toto nui i puta mai i te huaketo Ebola. Ko te nuinga o te tangata ka pangia e te pā atu ki ngā wai tinana, ngā mea huna me ngā paru o ngā tūroro, o ngā kararehe pangia rānei, ā, ko ngā whakaaturanga haumanu ko te kirika e puta mai ana, te toto me te kino o ngā okana maha. He teitei te reiti mate o te EHF, 50%-90%.
Hongere
| FAM | Waikawa karihi MP |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | Serum hou、Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/μL |
| Te motuhaketanga | Whakamahia ngā kete hei whakamātautau i ngā tohutoro kino a te kamupene, kia tutuki ai ngā hua i ngā whakaritenga. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96, me te Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Te matū tango e taunakitia ana: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nā Tiangen Biotech (Beijing) Co.,Ltd. Me tango kia rite ki ngā tohutohu, ā, ko te rōrahi tango e taunakitia ana mō te tauira he 140μL, ā, ko te rōrahi wairewa e taunakitia ana he 60μL.
Kōwhiringa 2.
Te matū tango e taunakitia ana: Kete Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Me tango kia rite ki ngā tohutohu. Ko te rōrahi tauira tango he 200μL, ā, ko te rōrahi wairewa e taunakitia ana he 80μL.
Kōwhiringa 3.
Ngā matū tango e taunakitia ana: Me tango te matū tango waikawa karihi (1000020261) me te pūnaha whakarite tauira aunoa a te High Throughput (MGISP-960) mā te BGI kia rite ki ngā tohutohu. Ko te rōrahi tango he 160μL, ā, ko te rōrahi wairewa e taunakitia ana he 60μL.
