14 Nga Momo o te Papillomavirus Tangata Tino Morearea (16/18/52 Pato)
ingoa hua
HWTS-CC019A-14 Momo o te Papillomavirus Tangata morearea nui (16/18/52 Pato) Kete Rapu Waikawa Nucleic(Fluorescence PCR)
Epidemiology
Kua kitea e nga rangahau ko nga mate HPV mau tonu me nga mate maha tetahi o nga tino take o te mate pukupuku pukupuku.I tenei wa, kei te ngaro tonu nga rongoa whai hua mo te mate pukupuku pukupuku na te HPV, no reira ko te kitenga wawe me te aukati i te mate pukupuku na te HPV te mea matua hei aukati i te mate pukupuku pukupuku.He mea nui ki te whakarite i tetahi whakamatautau tātaritanga etiology ngawari, motuhake me te tere mo te taatai haumanu me te maimoatanga o te mate pukupuku pukupuku.
Hongere
| Hongere | Momo |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te Mimi |
| Ct | ≤28 |
| LoD | 300 kape/mL |
| Tauwhāiti | Karekau he tauhohenga whakawhiti me etahi atu tauira rewharewha penei i te Rewharewha A, Rewharewha B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc me te DNA genomic tangata. |
| Nga taputapu e tika ana | MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu me BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
1.Tauira mimi
A: Tangohia1.4mL o te tauira mimi hei whakamatautau me te centrifuge i te 12000rpm mo te 5 meneti;makahia te supernatant (e taunakitia ana kia mau ki te 10-20μL supernatant mai i te raro o te ngongo centrifuge), taapirihia te 200μL o te reagent tuku tauira, a me whakahaere te tangohanga o muri mai i runga i nga tohutohu mo te whakamahi i te Tonotono & Micro-Test Sample Release Reagent (HWTS-3005-8).
B: Tangohia1.4mL o te tauira mimi hei whakamatautau me te centrifuge i te 12,000rpm mo te 5 meneti;makahia te supernatant (e taunaki ana kia mau ki te 10-20μL o te supernatant mai i te raro o te ngongo centrifuge), me te taapiri i te 200μL o te saline noa ki te whakamutu, hei tauira hei whakamatautau.Ko te tangohanga o muri ka taea te whakahaere me te Tohutono & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Tonotono & Whakamatau Moroiti Aunoa Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. i runga ano i nga tohutohus hei whakamahi.Ko te 80μL te rōrahi elution tūtohu.
C: Tangohia1.4mL o te tauira mimi hei whakamatautau me te centrifuge i te 12,000rpm mo te 5 meneti;makahia te supernatant (e taunaki ana kia mau ki te 10-20μL o te supernatant mai i te raro o te ngongo centrifuge), me te taapiri i te 200μL o te saline noa ki te whakamutu, hei tauira hei whakamatautau.Ko te tangohanga o muri ka taea te whakahaere meQIAamp DNA Mini Kit(51304) na QIAGEN or Tonotono & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Me tukatuka te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te rahinga tauira tangohanga he 200μL, a, ko te 80 te rahinga haunga e taunaki anaμL.
2. Tauira ūkui ū / ūkui tara
A: Tangohia te 1mL o te tauira kia whakamatauria ki te 1.5mLof ngongo centrifuge,acentrifuge i te 12000rpm mo te 5 meneti. Discard te supernatant (e taunaki ana kia mau ki te 10-20μL o te supernatant mai i te raro o te ngongo centrifuge), taapirihia te 100μL o te reagent tuku tauira, ka tango kia rite ki nga tohutohu mo te whakamahi i te Tonotono & Micro-Test Tauira Reagent Reagent ( HWTS-3005-8).
B: Ka taea te tangohanga me te Tohutono & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Tonotono & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. i runga i nga tohutohu mo te whakamahi.Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 80μL.
C: Ko te tangohanga ka taea te whakahaere me te QIAamp DNA Mini Kit(51304) na QIAGEN or Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Me tukatuka te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te rahinga tauira tangohanga he 200 μL, a ko te rōrahi elution e taunaki ana80μL.
3、Waepuu uwha/Waea Wahine
I mua i te whakatauira, whakamahia he miro hei muru ata i te taikaha o nga mea huna mai i te kopu, ka whakamahi i tetahi atu miro ūkui kua kuhu ki te otinga tiaki pūtau, te paraihe tauira pūtau kua whakahaehaehia ranei hei piri ki te mucosa cervical me te huri i te karaka kia 3-5 nga rauna kia whiwhi. nga pūtau kua werohia ki te kopu.Me ata tango i te miro, te paraihe ranei,akuhua ki roto i te ipuipu tauira me te 1mL o te tote noa. AI muri i te horoinga katoa, kothia kia whakamarokehia te ūkui miro, paraihia ranei ki te pakitara ngongo ka rukea, whakamauhia te potae ngongo, tohuhia te ingoa tauira (te nama ranei) ka pato ki te ngongo tauira.
