17 Momo HPV (16/18/6/11/44 Pato)
ingoa hua
HWTS-CC015 17 Momo Papillomavirus Tangata (16/18/6/11/44 Pato) Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te mate pukupuku servikal tetahi o nga pukupuku kino e tino kitea ana i roto i te waahanga whanau wahine. Kua kitea ko te mate HPV tohe me nga mate maha tetahi o nga take nui o te mate pukupuku pukupuku. I tenei wa kei te iti tonu nga rongoa whai hua mo te mate pukupuku pukupuku na te HPV. No reira, ko te kite wawe me te arai i te mate pukupuku na te HPV te mea matua hei aukati i te mate pukupuku pukupuku. Ko te whakarite i nga whakamatautau whakamaarama ngawari, motuhake me te tere mo nga tukumate he mea tino nui mo te taatai haumanu o te mate pukupuku pukupuku.
Hongere
| PCR-Ranu1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Mana whakahaere o roto |
| PCR-Ranu2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Mana whakahaere o roto |
Tawhā Hangarau
| Rokiroki | -18℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Tauira mimi, tauira ūkui waha wahine, tauira ūkui tara wahine |
| Ct | ≤28 |
| LoD | 300Topi/mL |
| Tauwhāiti | Karekau he tauhohenga whakawhiti ki te Ureaplasma urealyticum, Chlamydia trachomatis o te waahanga whanau, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mould, Gardnerella me etahi atu momo HPV kaore i kapi i te kete. |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent tangohanga Manakohia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor-300 HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Taapirihia te 200μL o te tote noa hei whakamutu i te pire i te taahiraa 2.1, katahi ka whakahaerehia te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te 80μL te rōrahi elution tūtohu.
Reagent tangohanga Manakohia: QIAamp DNA Mini Kit (51304) or Tonotono & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Taapirihia te 200μL o te tote noa ki te whakamutu i te pire i te taahiraa 2.1, katahi ka whakahaerehia te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te 200μL te rahinga tauira kua tangohia, ko te 100μL te rōrahi elution e taunaki ana.
Reagent tangohanga Manakohia: Tohutono & Moroiti-Whakamātautau Tauira Tuku Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Tāpirihia he 200μL o te tauira tuku reagent ki te whakatārewa i te pire i te taahiraa 2.1, katahi ka whakahaerehia te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga.
