15 Nga Momo o te Human Papillomavirus E6/E7 Gene mRNA

Whakaahuatanga Poto:

Ko tenei kete e whai ana ki te rapunga kounga o te 15 tino morearea tangata papillomavirus (HPV) E6/E7 taumata whakaputanga mRNA ira ira i roto i nga pūtau exfoliated o te cervix wahine.


Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-CC005A-15 Nga Momo o te Papillomavirus Tangata E6/E7 Ira ira MRNA Kit Rapu (Fluorescence PCR)

Epidemiology

Ko te mate pukupuku servikal tetahi o nga momo mate pukupuku wahine e tino kitea ana i te ao katoa, a, he tata te papillomavirus o te tangata (HPV), engari he iti noa te wahanga o nga mate HPV ka puta hei mate pukupuku.Ko te HPV morearea nui ka pangia nga pūtau epithelial cervical me te whakaputa e rua oncoproteins, E6 me E7.Ka taea e tenei pūmua te pa ki nga momo momo pūmua pūtau (pērā i te pūmua aukati puku pRB me te p53), ka whakaroa i te huringa pūtau, ka pa ki te whakahiato DNA me te pumau o te ira, me te whakararu i nga urupare parepare antiviral me te antitumor.

Hongere

Hongere Waehanga I whakamatauria te momo momo momo
FAM Pūrei Tauhohenga HPV 1 HPV16,31,33,35,51,52,58
VIC/HEX Ira β-actin tangata
FAM Pūrei Tauhohenga HPV 2 HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX Ira INS tangata

Tawhā Hangarau

Rokiroki Wai: ≤-18 ℃
Papa-ora 9 marama
Momo Tauira Te ūkui waha
Ct ≤38
CV <5.0%
LoD 500 kape/mL
Nga taputapu e tika ana Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturuWhakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere

QuantStudio®5 Pūnaha PCR Wā-tūturu

SLAN-96P Pūnaha PCR Wā-tūturu

LightCycler®480 Pūnaha PCR Wā-tūturu

LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu

MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha

BioRad CFX96 Pūnaha PCR Wā-tūturu

BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu

Rere Mahi

Reagent Tangohanga Manakohia: Tohutono & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me tino whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi .Ko te 50μL te rōrahi elution tūtohu.Ki te kore te tauira i tino keri, whakahokia ki te taahiraa 4 mo te kena ano.Na ka whakamatau kia rite ki nga tohutohu mo te whakamahi.

Reagent tangohanga Manakohia: RNAprep Pure Animal Tissue Tapeke RNA Extraction Kit (DP431).Me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi tino (I roto i te taahiraa 5, whawhati te kukū o te otinga mahi DNaseI, ara, tango 20μL o RNase-Free DNaseI (1500U) otinga kararehe ki roto i te ngongo centrifuge RNase-Free hou, tāpirihia te 60μL o te RDD buffer, ka uru ata).Ko te 60μL te rōrahi elution tūtohu.Ki te kore te tauira i tino keri, whakahokia ki te taahiraa 5 mo te kena ano.Na ka whakamatau kia rite ki nga tohutohu mo te whakamahi.


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