28 Ngā Momo Kete Kimi Waikawa Nukleika o te HPV (Fluorescence PCR)
Ingoa hua
28 Ngā Momo Kete Kimi Waikawa Nukleika o te HPV (Fluorescence PCR)
Te mate urutā
Ko te Human Papillomavirus (HPV) nō te whānau Papillomaviridae o tētahi huaketo DNA porowhita-rua, he iti te ngota, kāore i te takai, ā, e 8000 ngā takirua turanga (bp) te roa o te ira. Ka pangia te tangata e te HPV mā te whakapā tika, mā te whakapā ā-kore rānei ki ngā taonga kua poke, mā te tuku i te taatai rānei. Ehara i te mea he motuhake ki te tinana anake te huaketo, engari he motuhake hoki ki te kiko, ā, ka taea anake te pangia te kiri tangata me ngā pūtau epithelial mucosal, ka puta he momo papilloma, he kiri rānei i roto i te kiri tangata, ka nui haere te kino o te epithelium o te ara whakaputa uri.
Ko te mate pukupuku o te kōpū tetahi o ngā pukupuku kino tino kitea o te ara whakaputa uri wahine. Kua whakaatuhia e ngā rangahau ko ngā mate HPV pumau me ngā mate maha tetahi o ngā tino take o te mate pukupuku o te kōpū. I tēnei wā, kāore anō kia kitea he maimoatanga whai hua mō te mate pukupuku o te kōpū i puta mai i te HPV, nō reira ko te kitenga wawe me te aukati i te mate pukupuku o te kōpū i puta mai i te HPV te mea nui ki te aukati i te mate pukupuku o te kōpū. He mea nui te whakatū i tētahi whakamātautau tātaritanga take māmā, motuhake me te tere mō te tātaritanga haumanu me te maimoatanga o te mate pukupuku o te kōpū.
Hongere
| S/N | Hongere | Momo |
| PCR-Whakaranu1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Mana Whakahaere ā-roto | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Ngā Tawhā Hangarau
| Rokiroki | ≤ -18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Tauira mimi, Tauira ūkui waha wahine, Tauira ūkui tara wahine |
| CV | <5.0% |
| LoD | 300 Ngā Tārua/mL |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti me ētahi atu huaketo e pā ana ki te ara whakaputa uri me te DNA ira tangata. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu Applied Biosystems 7500, Ngā Pūnaha PCR Wā-Tūturu Tere Applied Biosystems 7500, Ngā Pūnaha PCR Wā-Tūturu QuantStudio®5, Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.), Ngā Pūnaha PCR Wā-Tūturu LightCycler®480, Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Pūnaha PCR Wā-Tūturu BioRad CFX96, Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96. |
Rerenga mahi: Tirohia te Kete IFU mō ētahi atu kōrero
Otinga 1 (Tangohanga waikawa karihi aunoa)
Otinga 2: (Mātū Whakamātautau Tauira Macro me Micro-Test)
Me whakarato ngā tauhohenga engari kāore i te whakaratohia
Te Pūhui Tukunga Tauira Macro & Micro-Test (HWTS-3005-8), Kete DNA/RNA Huakita Macro & Micro-Test (HWTS-3017) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Pou DNA/RNA Huakita Macro & Micro-Test (HWTS-3020-50).
E hiahiatia ana ngā mea e pau ana, engari kāore i te whakaratohia
1.5mL o ngā ngongo kore-DNase/RNase, ngā pito kore-DNase/RNase, ngā rīpene ngongo-8 mō te PCR, te miihini whakapūkara i runga tepu, te mīhini whakaranu rūrū i runga tepu.
