Borrelia Burgdorferi Waikawa Nucleic
ingoa hua
HWTS-OT068 Borrelia Burgdorferi Kete Rapu Waikawa Nucleic(Fluorescence PCR)
Tiwhikete
CE
Epidemiology
Ko te mate Lyme ka puta mai i te mate ki a Borrelia burgdorferi, ka tukuna te nuinga i waenga i nga kaihautu kararehe, i waenga i nga kararehe manaaki me te tangata ma nga tohu pakeke.Ka taea e te pathogen Borrelia burgdorferi te mate tangata Erythema chronicum migrans, me nga mate e pa ana ki nga punaha maha penei i te ngakau, te nerve, me te hononga, me etahi atu, he rereke nga whakaaturanga haumanu.E ai ki te whakawhanaketanga o nga akoranga mate, ka taea te wehea ki nga mate moata o te rohe, te mate kua horahia waenga me te mate mau tonu o te mutunga, he tino kino ki te hauora o te taupori.Na reira, i roto i te taatai haumanu o Borrelia burgdorferi, he mea nui ki te whakatu i tetahi tikanga ngawari, motuhake me te tere mo te taatai etiological o Borrelia burgdorferi.
Hongere
| FAM | DNA o Borrelia burgdorferi |
| VIC/HEX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | He tauira toto katoa |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kape/mL |
| Nga taputapu e tika ana | ABI 7500 Pūnaha PCR Wā-tūturu ABI 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
QIAamp DNA Blood Midi Kit na Qiagen (51185).It me unui roto i te tino tikangaki te ako, me te rōrahi elution tūtohu ko100μL.
Kōwhiringa 2.
TotoGDNA taitamarikiEkete tangohanga (DP318,Kao.: JingchangPūrere Pūrere20210062) na Tiangen Biochemical Technology (Beijing) Co., Ltd.. It me unui roto i te tino tikangaki te ako, me te rōrahi elution tūtohu ko100μL.
Kōwhiringa 3.
Wizard® Genomic DNA Purification Kit (A1120) na Promega.It me unui roto i te tino tikangaki te ako, me te rōrahi elution tūtohu ko100μL.
