Chlamydia Trachomatis, Neisseria Gonorrhoeae me Trichomonas vaginalis
ingoa hua
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae me Trichomonas vaginalis Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko Chlamydia trachomatis (CT) he momo moroiti prokaryotic he tino parapara i roto i nga pūtau eukaryotic.Ka wehewehea a Chlamydia trachomatis ki nga AK serotypes i runga i te tikanga serotype.Ko nga mate o te urogenital te nuinga ka puta mai i te trachoma biological variant DK serotypes, a ko te nuinga o nga tane e whakaatuhia ana ko te urethritis, ka taea te rongoa me te kore maimoatanga, engari ko te nuinga o ratou ka roa, ka kaha haere, ka taea te whakakotahi ki te epididymitis, proctitis, etc.
Hongere
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Mana o roto |
Tawhā Hangarau
| Rokiroki | -18℃ |
| Papa-ora | 12 marama |
| Momo Tauira | ūkui waha wahineūkui tara wahine，ūkui urethral tane |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Nga kape/mL |
| Tauwhāiti | Karekau he tauhohenga whakawhiti me etahi atu mate mate STD i waho o te awhe kitenga o te kete whakamatautau, penei i te Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex momo huaketo 1, Herpes simplex momo huaketo 2, Candida albicans, etc. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Reagent tangohanga e taunakitia ana: Pipette 1mL o te tauira kia whakamatauria ki te 1.5mL o te ngongo centrifuge DNase/RNase-kore, centrifuge i te 12000rpm mo te 3 meneti, makahia te supernatant me te pupuri i te rerenga.Taapirihia te 200µL o te tote noa ki te wai tote kia whakatarewa ano.Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me mahi te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te ruri tauira kua tangohia ko te 200µL, ko te 80µL te ruri whakahiato e taunakitia ana.
Kōwhiringa 2.
Reagent tangohanga e manakohia ana: Tangohanga Acid Nucleic, Kete Pure ranei (YDP302).Ko te tangohanga me tino rite ki nga tohutohu mo te whakamahi.Ko te 80µL te rahinga o te elution.
