Chlamydia Trachomatis, Neisseria Gonorrhoeae me Trichomonas vaginalis
Ingoa hua
HWTS-UR041 Kete Kimi Waikawa Nukleika mō te Chlamydia Trachomatis, Neisseria Gonorrhoeae me te Trichomonas vaginalis (PCR Fluorescence)
Te mate urutā
Ko te Chlamydia trachomatis (CT) he momo moroiti prokaryotic e pirinoa ana i roto i ngā pūtau eukaryotic. Kua wehea te Chlamydia trachomatis ki ngā momo AK e ai ki te tikanga momo. Ko ngā mate urogenital te nuinga o te wā e puta mai ana i ngā momo DK rerekē o te trachoma, ā, ko ngā tāne ka puta ko te urethritis, ka taea te whakaora me te kore rongoā, engari ko te nuinga o ēnei ka roa, ka kino haere i ētahi wā, ā, ka taea te hono atu ki te epididymitis, te proctitis, me ētahi atu.
Hongere
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Te mate pāwera Trichomonal |
| VIC/HEX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | -18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Te ūkui waha o te wahine,Te ūkui tara wahine,Te ūkui urethral tāne |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Ngā Tārua/mL |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti ki ētahi atu mate tuku iho i waho o te awhe kitea o te kete whakamātautau, pērā i te Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, me ētahi atu. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Te matū tango e taunakitia ana: Tāpirihia te 1mL o te tauira hei whakamātautau ki roto i te ngongo centrifuge kore-DNase/RNase 1.5mL, centrifuge i te 12000rpm mō te 3 meneti, maka atu te wai i roto, ā, puritia te wai i roto. Tāpirihia he 200µL o te tote noa ki te wai hei whakapūmau anō. Kete DNA/RNA Whānui Macro & Micro-Test (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me mahi te tango kia rite ki ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 200µL, ā, ko te rahinga wai i tūtohutia he 80µL.
Kōwhiringa 2.
Te matū tango e taunakitia ana: Kete Tango Waikawa Nucleic, Kete Purification rānei (YDP302). Me mahi te tango kia rite tonu ki ngā tohutohu whakamahi. Ko te rōrahi wairewa e taunakitia ana ko 80µL.
