Virus Dengue, Virus Zika me Chikungunya Virus Multiplex
ingoa hua
HWTS-FE040 Dengue Virus, Zika Virus me Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ko te kirika Dengue (DF), na te mate dengue huaketo (DENV), tetahi o nga mate hopuhopu arbovirus.Ko tana tikanga tuku ko Aedes aegypti me Aedes albopictus.Ko te nuinga o te DF kei roto i nga waahi ngaru me nga waahi iti.No flavivirus a DENV i raro i te flaviviridae, ka taea te whakarōpūtia ki te 4 serotypes i runga i te antigen mata.Ko nga whakaaturanga haumanu o te mate DENV te nuinga ko te mahunga, te kirikaa, te ngoikore, te whakanui i te kopuku lymph, te leukopenia me etahi atu, me te toto, te ohorere, te whara ate, te mate ranei i nga keehi kino.I nga tau tata nei, ko te huringa o te rangi, te noho taone nui, te tere tere o te mahi tuuruhi me etahi atu mea kua kaha ake te tere me te watea mo te tuku me te horapa o te DF, e arai atu ana ki te roha tonu o te waahi mate uruta o DF.
Hongere
| FAM | MP waikawa karihi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | -18℃ |
| Papa-ora | 9 marama |
| Momo Tauira | Serum hou |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kape/mL |
| Tauwhāiti | Ko nga hua whakamatautau pokanoa e whakaatu ana ka kore e neke ake te kukū o te bilirubin i roto i te serum i te 168.2μmol/ml, ko te kukū o te hemoglobin i puta mai i te hemolysis kaua e neke ake i te 130g/L, ko te kukū o te lipid toto kaua e neke ake i te 65mmol/ml, te tapeke IgG Ko te kukume i roto i te serum kaua e neke ake i te 5mg/mL, karekau he paanga ki te huaketo dengue, te huaketo Zika me te kitenga huaketo chikungunya.Ko te huaketo Hepatitis A, ko te Hepatitis B, ko te Hepatitis C, ko te Herpes, ko te Eastern equine encephalitis, ko te Hantavirus, ko te Bunya, ko te West Nile, ko nga tauira serum genomic tangata ka tohua mo te whakamatautau-whakawhitiwhiti, a ka whakaatu nga hua kaore he tauhohenga whakawhiti i waenganui i tenei kete me nga tukumate kua whakahuahia ake nei. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te rōrahi tauira tangohia ko 140μL, me te rōrahi elution tūtohu ko 60μL.
Kōwhiringa 2.
Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 80μL.
