Coxsackie Virus Momo A16 Waikawa Nucleic

Ko tenei kete ka tango i te Enzymatic Probe Isothermal Amplification (EPIA), me te hoahoa i nga tohu tuatahi me nga rangahau-a-papa RNA (rProbe) mo te rohe tino tiakina o Cox A16, me te taapiri i te Bst enzyme me te RNaseH enzyme i te wa ano, kei reira te taha maui me te Ko nga pito matau o te turanga RNA o te rProbe kua tapaina ki nga roopu rewharewha me te tinei.Whakamahia te ngohe DNA polymerase me te whenu displacement ngohe o Bst enzyme ki te whakanui ake i te whainga kia whakamatauria i te mahana tonu, ka taea e te enzyme RNaseH te wawahi i nga turanga RNA i runga i te mekameka ranu-probe-target, kia noho ai te roopu rewharewha me te tinei o te rProbe. ka wehea na reira ka rere ana.I tua atu, ko te kongakonga maui o te toenga rProbe RNA turanga ka taea te whakamahi hei tuatahi ki te toro atu ki te hanga hua, ka whakaemi ake i te hua.Kei te whakaemi tonu te tohu fluorescent me te hanganga o te hua, na reira ka mohio te kitenga o te waikawa nucleic target.