Te Huaketo Dengue, te Huaketo Zika me te Huaketo Chikungunya Multiplex
Ingoa hua
Kete Kimi Waikawa Matūriki HWTS-FE040 mō te Huaketo Dengue, te Huaketo Zika me te Huaketo Chikungunya (Fluorescence PCR)
Te mate urutā
Ko te kirika dengue (DF), e puta mai ana i te mate huaketo dengue (DENV), ko tētahi o ngā mate pāwera arbovirus tino horapa. Kei roto i tana ara tuku ko Aedes aegypti me Aedes albopictus. Ko te DF te mea tino horapa i ngā rohe wera me te haurua-mā-uru. Nō te flavivirus i raro i te flaviviridae te DENV, ā, ka taea te whakarōpū ki ngā momo momo e 4 e ai ki te antigen mata. Ko ngā whakaaturanga haumanu o te mate DENV ko te ānini, te kirika, te ngoikore, te whakanui i te kōpū lymph, te leukopenia me ētahi atu, me te toto, te ohorere, te whara ate, tae noa ki te mate i ngā take kino. I ngā tau tata nei, nā te huringa āhuarangi, te noho tāone, te tere o te whanaketanga o te tūruhi me ētahi atu āhuatanga kua tere ake, kua watea ake hoki ngā āhuatanga mō te tuku me te horapa o te DF, ka hua ake te whānui tonu o te rohe mate urutā o te DF.
Hongere
| FAM | Waikawa karihi DENV |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | -18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | Serum hou |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Ngā Tārua/mL |
| Te motuhaketanga | E whakaatu ana ngā hua o te whakamātautau whakararuraru, ina kore te kukū o te bilirubin i roto i te toto e neke ake i te 168.2μmol/ml, kāore te kukū hemoglobin i puta mai i te hemolysis e neke ake i te 130g/L, kāore te kukū lipid toto e neke ake i te 65mmol/ml, kāore te kukū IgG katoa i roto i te toto e neke ake i te 5mg/mL, kāore he pānga ki te kitenga o te huaketo dengue, te huaketo Zika, te huaketo chikungunya rānei. Ka tīpakohia te huaketo Hepatitis A, te huaketo Hepatitis B, te huaketo Hepatitis C, te huaketo Herpes, te huaketo Eastern hockey encephalitis, te Hantavirus, te huaketo Bunya, te huaketo West Nile me ngā tauira serum ira tangata mō te whakamātautau tauhohenga whakawhiti, ā, e whakaatu ana ngā hua kāore he tauhohenga whakawhiti i waenga i tēnei kete me ngā mate pukupuku kua whakahuatia i runga ake nei. |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Kete DNA/RNA Huakita TIANamp (YDP315-R), ā, me whakahaere te tangohanga kia rite ki ngā tohutohu whakamahi. Ko te rōrahi tauira i tangohia he 140μL, ā, ko te rōrahi wairewa i tūtohutia he 60μL.
Kōwhiringa 2.
Kete Whakamātautau Ira Whānui mō te Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ā, me whakahaere te tangohanga kia rite ki ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 200μL, ā, ko te rahinga wairewa i tūtohutia he 80μL.
