Virus Dengue, Virus Zika me Chikungunya Virus Multiplex
ingoa hua
HWTS-FE040 Dengue Virus, Zika Virus me Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ko te kirika Dengue (DF), na te mate dengue huaketo (DENV), tetahi o nga mate hopuhopu arbovirus. Ko tana tikanga tuku ko Aedes aegypti me Aedes albopictus. Ko te nuinga o te DF kei roto i nga waahi ngaru me nga waahi iti. No flavivirus a DENV i raro i te flaviviridae, ka taea te whakarōpūtia ki te 4 serotypes i runga i te antigen mata. Ko nga whakaaturanga haumanu o te mate DENV te nuinga ko te mahunga, te kirikaa, te ngoikore, te whakanui i te kopuku lymph, te leukopenia me etahi atu, me te toto, te ohorere, te whara ate, te mate ranei i nga keehi kino. I nga tau tata nei, ko te huringa o te rangi, te noho taone nui, te tere tere o te mahi tuuruhi me etahi atu mea kua kaha ake te tere me te watea mo te tuku me te horapa o te DF, e arai atu ana ki te roha tonu o te waahi mate uruta o DF.
Hongere
| FAM | DENV waikawa karihi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | -18℃ |
| Papa-ora | 9 marama |
| Momo Tauira | Serum hou |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 kape/mL |
| Tauwhāiti | Ko nga hua whakamatautau pokanoa e whakaatu ana ka kore e nui ake te kukū o te bilirubin i roto i te serum i te 168.2μmol / ml, ko te kukume hemoglobin i puta mai i te hemolysis kaore i neke ake i te 130g / L, ko te toto lipid toto e kore e neke atu i te 65mmol / ml, te tapeke IgG i roto i te serum kaua e neke ake i te 5mg / mL, kaore he paanga ki te huaketo chikunguka dengue ranei. Hepatitis A, Hepatitis B, Hepatitis C, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile huaketo me nga tauira serum genomic tangata ka tohua mo te whakamatautau whakawhiti-reactivity, a ko nga hua e whakaatu ana kaore he tauhohenga whakawhiti i waenga i tenei kete me nga tukumate kua whakahuahia i runga ake nei. |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi. Ko te rōrahi tauira tangohia ko 140μL, me te rōrahi elution tūtohu ko 60μL.
Kōwhiringa 2.
Tonotono & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006-3C) & H6TSB-3C) Micro-Test Med-Tech Co., Ltd., me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi. Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 80μL.
