Te huaketo Entero 71 (EV71)
Ingoa hua
Kete Kimi Waikawa Karihi HWTS-EV003- Enterovirus 71 (EV71) (PCR Whakamātautau Hiko)
Te mate urutā
Ko te mate ringa-waewae-waha he mate hopuhopu i puta mai i ngā enterovirus (EV). I tēnei wā, e 108 ngā momo serotype o ngā enterovirus kua kitea, e wehea ana ki ngā rōpū e whā: A, B, C me D. I roto i ēnei, ko te enterovirus EV71 me te CoxA16 ngā mate matua. Ko te nuinga o te mate ka pā ki ngā tamariki kei raro iho i te 5 tau te pakeke, ā, ka taea e ia te whakaputa i te herpes ki ngā ringa, waewae, waha me ētahi atu wāhanga. Ka pāngia he iti noa ngā tamariki e ngā raruraru pēnei i te myocarditis, te pulmonary edema, me te aseptic meningoencephalitis.
Hongere
| FAM | EV71 |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | He ūkui waha,Wai herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®480 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Ko te matū tango e taunakitia ana: Macro & Micro-Test Sample Release Agent (HWTS-3005-8), ā, me whakahaere te tango i runga i ngā tohutohu whakamahi. Ko ngā tauira i tangohia ko ngā ūkui o te waha, ngā tauira wai herpes rānei mai i ngā tūroro i kohia i te wāhi. Tāpirihia ngā ūkui i kohia ki roto i te Macro & Micro-Test Sample Release Agent (HWTS-3005-8), hurihia, ka whakaohokia kia pai, waiho ki te pāmahana rūma mō te 5 meneti, tangohia ki waho, kātahi ka hurihia kia pai te whakaranu, kia whiwhi ai i te RNA o ia tauira.
Kōwhiringa 2.
Te matū tango e taunakitia ana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ā, me whakahaere te tango kia rite ki ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 200µL, ā, ko te rahinga wairewa e taunakitia ana he 80µL.
Kōwhiringa3.
Te matū tango e taunakitia ana: QIAamp Viral RNA Mini Kit (52904) mā QIAGEN, TIANamp Virus DNA/RNA Kit rānei (YDP315-R), ā, me whakahaere te tango i runga anō i ngā tohutohu whakamahi. Ko te rahinga tauira i tangohia he 140μL, ā, ko te rahinga wairewa e taunakitia ana he 60µL.
