Enterovirus 71 (EV71)
ingoa hua
HWTS-EV003- Enterovirus 71 (EV71) Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te mate ringa-waewae-waha he mate hopuhopu na te enteroviruses (EV).I tenei wa, 108 nga momo serotypes o enteroviruses kua kitea, ka wehewehea ki nga roopu e wha: A, B, C me D. I roto i a raatau, ko te enterovirus EV71 me te CoxA16 nga pathogens matua.Ko te nuinga o te mate ka pa ki nga tamariki kei raro iho i te 5 tau te pakeke, ka pa te mate herpes ki nga ringa, waewae, waha me etahi atu waahanga.He tokoiti o nga tamariki ka puta nga raruraru penei i te myocarditis, te edema pulmonary, me te maningoencephalitis aseptic.
Hongere
| FAM | EV71 |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 9 marama |
| Momo Tauira | ūkui oropharyngealHerpes wai |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Kape/mL |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Reagent tangohanga e taunaki ana: Tonotono & Micro-Test Tauira Tuku Reagent (HWTS-3005-8), me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko nga tauira kua tangohia ko te oropharyngeal swabs, herpes wai tauira ranei mai i nga turoro i kohia i runga i te waahi.Taapirihia nga tapahi kua kohia ki roto i te Tohutono & Micro-Test Sample Reagent Reagent (HWTS-3005-8) tika, vortex me te uru pai, tuu ki te pāmahana rūma mo te 5 meneti, tangohia ki waho ka huri kia uru pai, kia whiwhi ai te RNA o ia tauira.
Kōwhiringa 2.
Reagent tangohanga Manakohia: Tohutono & Moroiti-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Tonotono & Micro-Test Tango Waikawa Nucleic Aunoa (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., me whakahaere te tangohanga kia rite ki te tohutohu mo te whakamahi.Ko te ruri tauira kua tangohia ko te 200µL, ko te 80µL te ruri whakahiato e taunakitia ana.
Kōwhiringa3.
Reagent tangohanga Manakohia: QIAamp Viral RNA Mini Kit (52904) na QIAGEN or TIANamp Virus DNA/RNA Kit (YDP315-R), a me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te 140μL te rahinga tauira i tangohia, me te 60μL te rōrahi elution tūtohu.
