Te Whakarerekētanga Ira Tangata BCR-ABL
Ingoa hua
Kete Kimi Huringa Ira HWTS-GE010A-Human BCR-ABL (Fluorescence PCR)
HWTS-GE016A-Kete Kimi Whakarerekētanga Ira Tangata BCR-ABL kua Maroke-tio (PCR Whakapūngao)
Te mate urutā
Ko te mate pūkahukahu myelogenous mau tonu (CML) he mate kino o ngā pūtau take hematopoietic. Neke atu i te 95% o ngā tūroro CML kei a rātou te chromosome Philadelphia (Ph) i roto i ō rātou pūtau toto. Ko te pathogenesis matua o te CML e whai ake nei: Ko te ira whakakotahi BCR-ABL ka hangaia mā te whakawhiti i waenga i te abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) i te ringa roa o te chromosome 9 (9q34) me te ira breakpoint cluster region (BCR) i te ringa roa o te chromosome 22 (22q11); ko te pūmua whakakotahi i whakakōrerohia e tēnei ira he tyrosine kinase (TK) te mahi, ā, ka whakahohe i ōna ara tohu o raro (pērā i te RAS, PI3K, me te JAK/STAT) hei whakatairanga i te wehenga pūtau me te aukati i te apoptosis pūtau, ka nui haere ngā pūtau kino, ā, ka puta te CML. Ko te BCR-ABL tētahi o ngā tohu tātaritanga nui o te CML. Ko te huringa hihiri o tōna taumata tuhinga he tohu pono mō te whakatau matapae o te leukemia, ā, ka taea te whakamahi hei matapae i te hokinga mai o te leukemia i muri i te maimoatanga.
Hongere
| FAM | Ira honohono BCR-ABL |
| VIC/HEX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | Wai: ≤-18℃ I te pōuri |
| Te roa o te oranga | Wai: 9 marama |
| Momo Tauira | Ngā tauira hinu wheua |
| LoD | 1000 Ngā Tārua/mL |
| Te motuhaketanga
| Kāore he tauhohenga whakawhiti me ētahi atu ira honohono TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, me PML-RARa |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio® 5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
