Te Whakarerekētanga Ira Tangata BRAF V600E
Ingoa hua
HWTS-TM007-Kete Kimi Whakarerekētanga Ira Tangata BRAF V600E (PCR Whakapūtanga Hiko)
Tiwhikete
CE/TFDA
Te mate urutā
Neke atu i te 30 ngā momo whakarerekētanga BRAF kua kitea, ā, tata ki te 90% o ēnei kei roto i te exon 15, te wāhi e kiia ana ko te whakarerekētanga V600E te whakarerekētanga tino noa, arā, ka whakarerekētia te thymine(T) i te tūranga 1799 i roto i te exon 15 ki te adenine (A), ka hua ake te whakakapinga o te valine (V) i te tūranga 600 ki te waikawa glutamic (E) i roto i te hua pūmua. He maha ngā wā ka kitea ngā whakarerekētanga BRAF i roto i ngā pukupuku kino pērā i te melanoma, te mate pukupuku colorectal, te mate pukupuku thyroid, me te mate pukupuku pūkahukahu. Ko te mārama ki te whakarerekētanga o te ira BRAF kua noho hei take mō te tirotiro i ngā rongoā EGFR-TKI me ngā rongoā e aro ana ki te whakarerekētanga ira BRAF i roto i te rongoā haumanu e aro ana ki ngā tūroro ka taea te whai hua.
Hongere
| FAM | Te whakarerekētanga V600E, te mana whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | ngā tauira kiko mate kua whakauruhia ki te paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Whakamahia ngā kete hei kimi i te mana whakahaere kounga LoD e rite ana. a) i raro i te papamuri momo-mohoao 3ng/μL, ka taea te kimi i te tere whakarerekētanga 1% i roto i te pūwero tauhohenga me te pumau; b) i raro i te tere whakarerekētanga 1%, ko te whakarerekētanga o te 1×103Ngā tārua/mL i te papamuri momo-mohoao o te 1×105Ka taea te kimi pūmau i ngā tārua/mL i roto i te pūwero tauhohenga; c) ka taea e te Pūwero Tauhohenga IC te kimi i te rohenga kitenga iti rawa o te mana whakahaere kounga SW3 o te mana whakahaere ā-roto o te kamupene. |
| Ngā Taputapu e Pā Ana: | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-TūturuPūnaha Koiora Whakamahia 7300 PCR Wā-Tūturu Ngā Pūnaha, Ngā Pūnaha PCR Wā-Tūturu QuantStudio® 5 Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha PCR Wā-Tūturu BioRad CFX96 |
Rere Mahi
Ngā matū tango e taunakitia ana: Kete Tisu QIAamp DNA FFPE a QIAGEN (56404), Kete Tango Tere o te Tisu Paraffin-embedded (DP330) i hangaia e Tiangen Biotech (Beijing) Co., Ltd.
