Te Whakarerekētanga Ira Tangata EML4-ALK
Ingoa hua
Kete Kimi Huringa Ira HWTS-TM006-Human EML4-ALK (PCR Whakapūtanga)
Tiwhikete
TFDA
Te mate urutā
E whakamahia ana tēnei kete hei kimi i ngā momo whakarerekētanga ira 12 o te ira whakakotahitanga EML4-ALK i roto i ngā tauira o ngā tūroro matepukupuku pūkahukahu tangata ehara i te pūtau iti i roto i te vitro. Ko ngā hua whakamātautau he tohutoro haumanu anake, kaua hoki e whakamahia hei pūtake anake mō te maimoatanga takitahi o ngā tūroro. Me whakatau whānui ngā tākuta hauora i ngā hua whakamātautau i runga i ngā āhuatanga pēnei i te āhua o te tūroro, ngā tohu rongoā, te urupare maimoatanga, me ētahi atu tohu whakamātautau taiwhanga. Ko te matepukupuku pūkahukahu te pukupuku kino tino noa o te ao, ā, 80%~85% o ngā take he matepukupuku pūkahukahu ehara i te pūtau iti (NSCLC). Ko te whakakotahitanga ira o te pūmua-rite-4 e hono ana ki te microtubule echinoderm (EML4) me te lymphoma kinase anaplastic (ALK) he whāinga hou i roto i te NSCLC, kei roto i ngā roopu P21 me P23 tangata i runga i te chromosome 2 te EML4 me te ALK, ā, e wehea ana e te 12.7 miriona ngā takirua turanga. Neke atu i te 20 ngā momo whakakotahitanga kua kitea, ā, i roto i ēnei ko ngā momo whakakotahitanga 12 i roto i te Ripanga 1 he mea noa, ko te momo whakakotahitanga 1 (E13; A20) te mea tino noa, ā, whai muri ko ngā momo whakakotahitanga 3a me 3b (E6; A20), e 33% me te 29% o ngā tūroro me te ira whakakotahitanga EML4-ALK NSCLC, ia. Ko ngā aukati ALK e tohuhia ana e Crizotinib he rongoā arotahi-ngota iti i whakawhanakehia mō ngā whakarerekētanga whakakotahitanga ira ALK. Mā te aukati i te mahi o te rohe ALK tyrosine kinase, ka aukati i ōna ara tohu rerekē i raro iho, ka aukati i te tipu o ngā pūtau pukupuku, kia tutuki ai te rongoā arotahi mō ngā pukupuku. Kua whakaatuhia e ngā rangahau haumanu he nui ake i te 61% te tere whai hua o Crizotinib i roto i ngā tūroro me ngā whakarerekētanga whakakotahitanga EML4-ALK, engari kāore he pānga ki ngā tūroro momo-mohoao. Nō reira, ko te kitenga o te whakarerekētanga whakakotahitanga EML4-ALK te kaupapa me te pūtake mō te ārahi i te whakamahinga o ngā rongoā Crizotinib.
Hongere
| FAM | Pūnaha tauhohenga 1, 2 |
| VIC(HEX) | Pūtāhui tauhohenga 2 |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | ngā tauira kiko mate, ngā tauira wāhanga rānei kua whakauruhia ki te paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Ka taea e tēnei kete te kite i ngā whakarerekētanga hanumi tae noa ki te 20 tārua. |
| Ngā Taputapu e Pā Ana: | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu SLAN ®-96P Ngā Pūnaha PCR Wā-Tūturu a QuantStudio™ 5 Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Kete RNeasy FFPE (73504) nā QIAGEN, Kete Tango RNA Katoa o ngā Wāhanga Tisu kua whakauruhia ki te Paraffin (DP439) nā Tiangen Biotech (Beijing) Co.,Ltd.
