Tangata PML-RARA Fusion Gene Mutation
ingoa hua
HWTS-TM017ATangata PML-RARA Fusion Gene Mutation Kit Detection (Fluorescence PCR)
Epidemiology
Ko te Acute promyelocytic leukemia (APL) he momo motuhake o te acute myeloid leukemia (AML). Tata ki te 95% o nga turoro APL e haere tahi ana me te huringa cytogenetic motuhake, ara ko t(15;17)(q22;q21), e hanga ana i te ira PML i runga i te chromosome 15 me te retinoic acid receptor α gene (RARA) i runga i te chromosome 17 ka honoa hei hanga i te ira whakakotahitanga PML-RARA. Na te rereke o nga waahi pakaru o te ira PML, ka taea te wehewehe i te ira whakakotahitanga PML-RARA ki te momo roa (momo L), momo poto (momo S) me te momo rereke (momo V), e tata ana ki te 55%, 40% me te 5%.
Hongere
| FAM | ira whakakotahitanga PML-RARA |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ I te pouri |
| Papa-ora | 9 marama |
| Momo Tauira | wheua wheua |
| CV | <5.0％ |
| LoD | 1000 kape/mL. |
| Tauwhāiti | Karekau he tauhohenga whakawhiti me etahi atu ira hanumi BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, me TEL-AML1 ira whakakotahi |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent tangohanga e manakohia ana: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Me whakahaere te tangohanga kia rite ki te IFU.
