Te Whakarerekētanga Ira PML-RARA Tangata
Ingoa hua
HWTS-TM017AKete Kimi Whakarerekētanga Ira Whakakotahitanga PML-RARA Tangata (PCR Whakapūtanga)
Te mate urutā
Ko te mate pūkahukahu promyelocytic acute (APL) he momo motuhake o te mate pūkahukahu myeloid acute (AML). Tata ki te 95% o ngā tūroro APL e haere tahi ana me te huringa cytogenetic motuhake, arā, ko te t(15;17)(q22;q21), e honoa ai te ira PML i runga i te chromosome 15 me te ira α receptor waikawa retinoic (RARA) i runga i te chromosome 17 hei hanga i te ira whakakotahitanga PML-RARA. Nā te rerekētanga o ngā pūwāhi pakaru o te ira PML, ka taea te wehewehe i te ira whakakotahitanga PML-RARA ki te momo roa (momo L), te momo poto (momo S) me te momo rerekē (momo V), e 55%, 40% me te 5% te nui.
Hongere
| FAM | Ira honohono PML-RARA |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ I te pōuri |
| Te roa o te oranga | 9 marama |
| Momo Tauira | hinu wheua |
| CV | <5.0% |
| LoD | 1000 Ngā Tārua/mL. |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti me ētahi atu ira whakakotahi BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, me ngā ira whakakotahi TEL-AML1 |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Me whakahaere te tango kia rite ki te IFU.
