Te Whakarerekētanga Ira ROS1 Tangata
Ingoa hua
HWTS-TM009-Kete Kimi Whakarerekētanga Ira ROS1 Tangata (PCR Whakapūtanga)
Tiwhikete
CE
Te mate urutā
Ko te ROS1 he tyrosine kinase whakawhiti-membrane o te whānau kaiwhakawhiwhi insulin. Kua whakaūtia te ira whakakotahi ROS1 hei ira nui anō mō te mate pukupuku pūkahukahu ehara i te pūtau iti. Hei māngai mō tētahi momo ngota ngota ahurei hou, ko te auau o te ira whakakotahi ROS1 i roto i te NSCLC. Tata ki te 1% ki te 2% ka huri te nuinga o te ROS1 i roto i ōna exons 32, 34, 35 me te 36. I muri i te whakakotahitanga ki ngā ira pēnei i te CD74, EZR, SLC34A2, me te SDC4, ka haere tonu tana whakahohe i te rohe ROS1 tyrosine kinase. Ka taea e te ROS1 kinase kua whakahohehia te whakahohe i ngā ara tohu o raro pēnei i te RAS/MAPK/ERK, PI3K/Akt/mTOR, me te JAK3/STAT3, ā, ka uru atu ki te tipu, te rerekētanga me te metastasis o ngā pūtau pukupuku, me te mate pukupuku. I roto i ngā whakarerekētanga ROS1, ko te CD74-ROS1 te 42%, ko te EZR te 15%, ko te SLC34A2 te 12%, ā, ko te SDC4 te 7%. Kua whakaatuhia e ngā rangahau he rite te pae here ATP o te rohe whakaterenga o te ROS1 kinase me te pae here ATP o te ALK kinase ki te 77%, nō reira he mārama te pānga whakaora o te aukati ngota iti o te ALK tyrosine kinase crizotinib me ētahi atu i roto i te maimoatanga o te NSCLC me te whakarerekētanga ROS1. Nō reira, ko te kitenga o ngā whakarerekētanga ROS1 te kaupapa me te pūtake mō te ārahi i te whakamahinga o ngā rongoā crizotinib.
Hongere
| FAM | Pūwero Tauhohenga 1, 2, 3 me te 4 |
| VIC(HEX) | Pūtāhui Tauhohenga 4 |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | kiko mate kua whakauruhia ki te parafini, ngā tauira tapatapahi rānei |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Ka taea e tēnei kete te kite i ngā whakarerekētanga hanumi tae noa ki te 20 tārua. |
| Ngā Taputapu e Pā Ana: | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-TūturuNgā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu SLAN ®-96P Ngā Pūnaha PCR Wā-Tūturu a QuantStudio™ 5 Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Kete RNeasy FFPE (73504) mai i QIAGEN, Kete Tango RNA Katoa o te Wāhanga Tisu Whakauru Paraffin (DP439) mai i Tiangen Biotech (Beijing) Co.,Ltd.
