Tauira Tukunga Tauira
Ingoa hua
Kaiwhakaora Tukunga Tauira Macro me Micro-Test
Tiwhikete
CE, FDA, NMPA
Ngā wāhanga matua
| Ingoa | Ngā wāhanga matua | Wāhangangā whakaritenga | Te rahinga |
| Tukunga Tauiramatū matū | Dithiothreitol, konutai dodecylte whanariki (SDS), te aukati RNase,te matū horoi, te wai parakore | 0.5mL/Ipu | 50 Pounamu |
Kia mōhio: Kāore e taea te whakawhiti i ngā wāhanga o ngā puranga kete rerekē.
Ngā tikanga rokiroki me te roa o te wā
Penapenahia, ā, kawehia ki te pāmahana rūma. E 24 marama te roa o te oranga.
Ngā taputapu e tika ana
Ngā taputapu me ngā taputapu i te wā e tukatuka ana i te tauira, pērā i ngā pipettes, ngā mīhini whakaranu vortex,ngā kaukau wai, me ētahi atu
Ngā whakaritenga tauira
Ngā ūkui waha, ngā ūkui ihu hou kua kohia.
Te tika
Ina whakamahia tēnei kete mō te tango mai i te CV tohutoro tika ā-whare mō ngā tāruarua 10, kāore te tauwehenga rerekētanga (CV, %) o te uara Ct e neke atu i te 10%.
Te rerekētanga i waenga i ngā puranga
Ina whakamatautauria te tohutoro tika o roto i runga i ngā puranga kete e toru kei raro i te whakamātautau whakaputa i runga i te tangohanga anō, ā, kāore te tauwehenga rerekētanga (CV, %) o te uara Ct e neke atu i te 10%.
Whakatairite mahi
● Te whakataurite i te whai huatanga o te tango
| Te whakataurite i te whai huatanga o te tikanga mō ngā pirepire aukume me te kaiwhakaputa tauira | ||||
| te arotahi | tikanga mō ngā pirepire aukume | kaiwhakaputa tauira | ||
| kākahu | N | kākahu | N | |
| 20000 | 28.01 | 28.76 | 28.6 | 29.15 |
| 2000 | 31.53 | 31.9 | 32.35 | 32.37 |
| 500 | 33.8 | 34 | 35.25 | 35.9 |
| 200 | 35.25 | 35.9 | 35.83 | 35.96 |
| 100 | 36.99 | 37.7 | 38.13 | kāore i te wehe |
He rite tonu te whai huatanga o te tangohanga o te kaiwhakaputa tauira ki te tikanga o ngā piripiri aukume, ā, ko te kukū o te mate huaketo ka eke ki te 200Copy/mL.
● Te whakataurite i te uara CV
| Te āheinga ki te tukurua i te tangohanga tauira | ||
| kukū: 5000 Ngā Tārua/mL | ORF1ab | N |
| 30.17 | 30.38 | |
| 30.09 | 30.36 | |
| 30.36 | 30.26 | |
| 30.03 | 30.48 | |
| 30.14 | 30.45 | |
| 30.31 | 30.16 | |
| 30.38 | 30.7 | |
| 30.72 | 30.79 | |
| CV | 0.73% | 0.69% |
I te whakamātautau i te 5,000 kape/mL, ko te CV o orFab me N he 0.73% me te 0.69%, ia.
