Mycobacterium Tuberculosis Waikawa Nucleic and Rifampicin(RIF), Ātete(INH)

Whakaahuatanga Poto:

Ka whakamahia tenei kete mo te rapunga kounga in vitro o Mycobacterium tuberculosis DNA i roto i te sputum tangata, ahurea totoka (LJ Waenga) me te ahurea wai (MGIT Waenga), wai horoi bronchial, me nga huringa i roto i te rohe codon waikawa amino 507-533 (81bp, rifampicin resistance te rohe whakatau) o te ira rpoipouri rpob. i roto i nga waahi rerekee matua o te Mycobacterium tuberculosis isoniazid resistance.He awhina ki te tātaritanga o te mate Mycobacterium tuberculosis, a ka kitea nga ira atete matua o te rifampicin me te isoniazid, e awhina ana ki te mohio ki te aukati tarukino o Mycobacterium tuberculosis i pangia e te manawanui.

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-RT147 Mycobacterium Tuberculosis Waikawa Nucleic and Rifampicin(RIF), (INH) Kete Rapu (Ana Rewa)

Epidemiology

Ko te Mycobacterium tuberculosis, ko te Tubercle bacillus (TB), ko te huakita pathogenic e puta ai te mate kohi, a, i tenei wa, ko nga raau taero anti-tuberculosis rarangi tuatahi ko te isoniazid, rifampicin me te ethambutol, aha atu.^[1]. Heoi, na te he o te whakamahi i nga raau taero anti-tuberculosis me nga ahuatanga o te hanganga pakitara o te mycobacterium tuberculosis ake, kua whakawhanakehia e te mycobacterium tuberculosis te aukati i nga raau taero ki nga raau taero anti-tuberculosis, a ko te ahua tino kino ko te mate kohi multidrug-resistant tuberculosis (MDR-TB), he ātete ki nga rongoa e rua tino noa me te whai hua, rifampicin me te rifampicin.^[2].

Ko te raruraru o te aukati i nga raau taero mate kohi kei nga whenua katoa i rangahauhia e te WHO. Hei whakarato i nga mahere maimoatanga tika mo nga turoro mate kohi, he mea tika ki te kite i te aukati ki nga raau taero anti-tuberculosis, ina koa te aukati rifampicin, kua waiho hei waahanga tātaritanga e taunakihia ana e te WHO mo te maimoatanga o te mate kohi.^[3]. Ahakoa ko te kitenga o te parenga rifampicin he rite tonu ki te kitenga o te MDR-TB, ko te kite i te parenga rifampicin ka kore e aro ki nga turoro me te INH mono-atete (e tohu ana i te parenga ki te isoniazid engari he tairongo ki te rifampicin) me te rifampicin mono-atete (te aro ki te isoniazid engari he ātete ki te rifampicin), tera pea ka tukuna nga turoro ki te maimoatanga tuatahi kaore i te tika. No reira, ko nga whakamatautau aukati isoniazid me rifampicin he whakaritenga iti rawa i roto i nga kaupapa whakahaere DR-TB katoa^[⁴^].

Tawhā Hangarau

Rokiroki	≤-18 ℃
Papa-ora	12 marama
Momo Tauira	Tauira Sputum, Tikanga Toka (LJ Waenga), Ahurea Wai (MGIT Waenga)
CV	<5.0%
LoD	Ko te LoD o te kete mo te kimi Mycobacterium tuberculosis he 10 huakita/mL;ko te LoD o te kete mo te kimi momo mohoao rifampicin me te momo mutant he 150 huakita/mL; Ko te LoD o te kete mo te kimi momo mohoao isoniazid me te momo mutant he 200 huakita/mL.
Tauwhāiti	1) Karekau he tauhohenga whakawhiti i te wa e whakamahi ana i te kete ki te kite i te DNA genomic tangata (500ng), etahi atu momo 28 o te tukumate manawa, me te 29 momo mycobacteria kore-tuberculous (e whakaatuhia ana i te Ripanga 3).2) Karekau he tauhohenga whakawhiti i te wa e whakamahi ana i te kete ki te kite i nga waahi rerekee o etahi atu ira whakararu o te rifampicin me te isoniazid tairongo Mycobacterium tuberculosis (penei i te Ripanga 4).3) Nga matū pokanoa noa i roto i nga tauira ka whakamatauria, penei i te rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), (0mg/L), pyrazinamide (45mg/L), (0mg/L), 5mg/L. nga raau taero, kaore he paanga ki nga hua whakamatautau kete.
Nga taputapu e tika ana	SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Pūnaha PCR Wā-tūturu