Te Waikawa me te Rifampicin (RIF), te Ātete (INH) o te Mycobacterium Tuberculosis

Whakaahua Matua o te Mycobacterium Tuberculosis Nucleic Acid and Rifampicin (RIF), Resistance (INH)

Whakaahuatanga Poto:

E whakamahia ana tēnei kete mō te kimi i te DNA Mycobacterium tuberculosis i roto i te huakita tangata, te ahurea totoka (LJ Medium) me te ahurea wai (MGIT Medium), te wai horoi pūkahukahu, me ngā whakarerekētanga i roto i te rohe codon waikawa amino 507-533 (81bp, rohe whakatau ātete rifampicin) o te ira rpoB o te Mycobacterium tuberculosis rifampicin resistance, me ngā whakarerekētanga i roto i ngā wāhi whakarerekētanga matua o te Mycobacterium tuberculosis isoniazid resistance. Ka āwhina i te tautuhi i te mate Mycobacterium tuberculosis, ā, ka kitea ngā ira ātete matua o te rifampicin me te isoniazid, e āwhina ana ki te mārama ki te ātete rongoā o te Mycobacterium tuberculosis i pangia e te tūroro.

Tukua mai he īmēra ki a mātou

Taipitopito Hua

Ngā Tohu Hua

Ingoa hua

Kete Kimi HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid me te Rifampicin (RIF), (INH) (Pīkoi Rewa)

Te mate urutā

Ko te Mycobacterium tuberculosis, ko te ingoa poto ko te Tubercle bacillus (TB), te huakita mate e hua ai te mate kohi, ā, i tēnei wā, ko ngā rongoā ārai-kohi e whakamahia whānuitia ana ko te isoniazid, rifampicin me te ethambutol, me ētahi atu.^[1]Heoi, nā te whakamahinga hē o ngā rongoā ārai-mate kohi me ngā āhuatanga o te hanganga pakitara pūtau o te mycobacterium tuberculosis tonu, kua whanake te ātete rongoā o te mycobacterium tuberculosis ki ngā rongoā ārai-mate kohi, ā, ko tētahi momo tino morearea ko te mate kohi ātete-maha-rongoā (MDR-TB), e ātete ana ki ngā rongoā e rua e tino kitea ana, e tino whai hua ana hoki, arā, te rifampicin me te isoniazid.^[2].

Kei ngā whenua katoa i rangahauhia e te WHO te raruraru o te ātete ki ngā rongoā kohi. Hei whakarato i ngā mahere maimoatanga tika ake mō ngā tūroro kohi, me kitea te ātete ki ngā rongoā ārai-kohi, inā koa te ātete ki te rifampicin, kua waiho hei taahiraa tātaritanga e taunakitia ana e te WHO mō te rongoā kohi.^[3]Ahakoa he tata rite te kitenga o te ātete ki te rifampicin ki te kitenga o te MDR-TB, ko te kimi noa i te ātete ki te rifampicin ka warewarehia ngā tūroro he INH ātete-mono (e pā ana ki te ātete ki te isoniazid engari he tairongo ki te rifampicin) me te rifampicin ātete-mono (tairongo ki te isoniazid engari he ātete ki te rifampicin), ka arahi pea ki ngā tūroro ki ngā tikanga maimoatanga tuatahi kāore i te tika. Nō reira, ko ngā whakamātautau ātete ki te isoniazid me te rifampicin ngā whakaritenga iti rawa e hiahiatia ana i roto i ngā kaupapa whakahaere DR-TB katoa.^[⁴^].

Ngā Tawhā Hangarau

Rokiroki	≤-18℃
Te roa o te oranga	12 marama
Momo Tauira	Tauira Hupa, Ahurea Totoka (Waenga LJ), Ahurea Wai (Waenga MGIT)
CV	<5.0%
LoD	Ko te LoD o te kete mō te kimi i te Mycobacterium tuberculosis he 10 huakita/mL;Ko te LoD o te kete mō te kimi i te rifampicin momo mohoao me te momo whakarerekētanga he 150 huakita/mL; Ko te LoD o te kete mō te kimi i te momo isoniazid mohoao me te momo mutant he 200 huakita/mL.
Te motuhaketanga	1) Kāore he tauhohenga whakawhiti i te whakamahinga o te kete hei kimi i te DNA ira tangata (500ng), ētahi atu momo mate manawa e 28, me ngā momo mycobacteria kāore i te mate kohi e 29 (e whakaaturia ana i te Ripanga 3).2) Kāore he tauhohenga whakawhiti i te wā e whakamahia ana te kete hei kimi i ngā wāhi whakarerekētanga o ētahi atu ira ātete-rongoa o te rifampicin me te Mycobacterium tuberculosis e tairongo ana ki te isoniazid (e whakaaturia ana i te Ripanga 4).3) Kāore he pānga o ngā matū e pokanoa ana i roto i ngā tauira hei whakamātautau, pērā i te rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), ki ngā hua whakamātautau o te kete.
Ngā Taputapu e Pā Ana	Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.), Pūnaha PCR Wā-Tūturu BioRad CFX96