Mycobacterium Tuberculosis Waikawa Nucleic and Rifampicin(RIF)，Isoniazid Resistance(INH)
ingoa hua
HWTS-RT147 Mycobacterium Tuberculosis Waikawa Nucleic and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)
Epidemiology
Ko te Mycobacterium tuberculosis, ko te Tubercle bacillus (TB), ko te huakita pathogenic e puta ai te mate kohi.I tenei wa, ko nga raau taero anti-tuberculosis tuatahi e whakamahia ana ko te isoniazid, rifampicin me te ethambutol, me etahi atu. Heoi, na te he o te whakamahi i nga raau taero anti-mate kohi me nga ahuatanga o te hanganga pakitara o te mycobacterium tuberculosis, ka whakawhanake te mycobacterium tuberculosis i te aukati i nga raau taero ki nga raau taero anti-tuberculosis, e kawe mai ana i nga wero nui ki te aukati me te maimoatanga o te mate kohi.
Hongere
| Ingoa Whainga | Kairīpoata | Whakamutua | ||
| Tauhohenga BufferA | Tauhohenga BufferB | Tauhohenga BufferC | ||
| rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | Karekau |
| rpoB 520-527 | rpoB 527-533 | / | CY5 | Karekau |
| / | / | Mana whakahaere o roto | HEX(VIC) | Karekau |
| Tauhohenga BufferD | Kairīpoata | Whakamutua |
| InhA rohe kaiwhakatairanga -15C>T, -8T>A, -8T>C | FAM | Karekau |
| KatG 315 codon 315G>A、315G>C | CY5 | Karekau |
| AhpC rohe kaiwhakatairanga -12C>T, -6G>A | ROX | Karekau |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | huka |
| CV | ≤5.0% |
| LoD | Ko te LoD o te mycobacterium tuberculosis tohutoro motu he 50 huakita/mL.He 2×10 te LoD o te momo mohoao-atete rifampicin mohoao3huakita/mL, me te LoD o te momo mutant he 2×103huakita/mL.Ko te LoD o te momo mohoao huakita atete isoniazid he 2x103huakita/mL, me te LoD o te huakita mutant he 2x103huakita/mL. |
| Tauwhāiti | Ko nga hua o te whakamatautau whiti i whakaatu karekau he tauhohenga whiti i te kitenga o te ira tangata, etahi atu mate mate kohi kore kohi me te pneumonia tukumate ki tenei kete;Karekau he tauhohenga whakawhiti i kitea i nga waahi rerekee o etahi atu ira atete ki te tarukino i roto i te momo mohoao Mycobacterium tuberculosis. |
| Nga taputapu e tika ana | SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Pūnaha PCR Wā-tūturu, Hangarau Hangzhou Bioer QuantGene 9600 Pūnaha PCR Wā-tūturu, QuantStudio®5 Pūnaha PCR Wā-tūturu.
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Tapeke PCR Solution
