Mycoplasma Pneumoniae (MP)
Ingoa hua
Kete Kimi Waikawa Matū HWTS-RT024 Mycoplasma Pneumoniae（MP） (PCR Whakaata)
Te mate urutā
Ko te Mycoplasma pneumoniae (MP) he momo moroiti prokaryotic iti rawa, kei waenganui i te huakita me te huaketo, he hanganga pūtau engari kāore he pakitara pūtau. Ko te MP te nuinga o te mate manawa o te tangata, inā koa i roto i ngā tamariki me ngā taiohi. Ka taea e ia te mate mycoplasma tangata, te mate manawa o ngā tamariki me te niumōnia rerekē. He rerekē ngā whakaaturanga haumanu, ko te nuinga he maremare nui, he kirika, he makariri, he ānini, he korokoro mamae. Ko ngā mate manawa o runga me te niumōnia pūkahukahu ngā mea tino noa. Ka taea e ētahi tūroro te whakawhanake mai i te mate manawa o runga ki te niumōnia kino, ka pāngia e te mamae manawa nui me te mate.
Hongere
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Hupa, ūkui waha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Ngā Tārua/mL |
| Te motuhaketanga | a) Tauhohenga whakawhiti: kāore he tauhohenga whakawhiti ki te Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, huaketo rewharewha A, huaketo rewharewha B, huaketo parainfluenza momo I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus me te human genomic acid. b) Te kaha ārai-whakararuraru: kāore he whakararuraru i te wā i whakamatautauria ai ngā matū whakararuraru me ēnei kukū: te hemoglobin (50mg/L), te bilirubin (20mg/dL), te mucin (60mg/mL), te toto tangata 10% (v/v), te levofloxacin (10μg/mL), te moxifloxacin (0.1g/L), te gemifloxacin (80μg/mL), te azithromycin (1mg/mL), te clarithromycin (125μg/mL), te erythromycin (0.5g/L), te doxycycline (50mg/L), te minocycline (0.1g/L). |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer) MA-6000 Pūrere Huringa Wera Tau-Wā-Tūturu (Suzhou Molarray Co., Ltd.) Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
(1) Tauira Huware
Te matū tango e taunakitia ana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tāpirihia he 200µL o te tote noa ki te matū kua tukatukahia. Me mahi te tango i muri mai kia rite ki te tohutohu whakamahi. Ko te rōrahi wairewa e taunakitia ana ko te 80µL. Te matū tango e taunakitia ana: Nucleic Acid Extraction, Purification Reagent rānei (YDP315-R). Me mahi te tango kia rite tonu ki te tohutohu whakamahi. Ko te rōrahi wairewa e taunakitia ana ko te 60µL.
(2) Te ūkui waha
Te matū tango e taunakitia ana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me mahi te tango i runga i ngā tohutohu whakamahi. Ko te rōrahi tango tauira e taunakitia ana ko 200µL, ā, ko te rōrahi wairewa e taunakitia ana ko 80µL. Te matū tango e taunakitia ana: QIAamp Viral RNA Mini Kit (52904) Nucleic Acid Extraction, Purification Reagent rānei (YDP315-R). Me mahi te tango i runga i ngā tohutohu whakamahi. Ko te rōrahi tango tauira e taunakitia ana ko 140µL, ā, ko te rōrahi wairewa e taunakitia ana ko 60µL.
