Mycoplasma Pneumoniae （MP）
ingoa hua
HWTS-RT024 Mycoplasma Pneumoniae（MP） Kete Rapu Waikawa Nucleic(Fluorescence PCR)
Epidemiology
Ko te Mycoplasma pneumoniae (MP) he momo moroiti prokaryotic iti rawa, kei waenga i te huakita me te huaketo, me te hanganga pūtau engari kaore he pakitara o te pūtau.Ko te nuinga o nga mema o te MP e pa ana ki te mate rewharewha o te tangata, ina koa ki nga tamariki me nga taiohi.Ka taea e te mate pukupuku mycoplasma tangata, te mate rewharewha o nga tamariki me te pneumonia atypical.He maha nga ahuatanga o te haumanu, ko te nuinga he mare nui, he kirikaa, he makariri, he mahunga, he korokoro.Ko te mate rewharewha o runga me te pneumonia bronchial te nuinga.Ka taea e etahi o nga turoro te mate mai i te mate rewharewha o runga ki te mate kawa kawa, ka pa te mamae o te manawa me te mate.
Hongere
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Sputum, pania oropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 kape/mL |
| Tauwhāiti | a） Whakahohenga whiti: karekau he tauhohenga whiti me te Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legion baconella Psetobainosa, Legionella ba. rewharewha A huaketo , Irewharewha B huaketo, Parainfluenza wheori momo I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial wheori me te tangata genomic waikawa nucleic. b）Te kaha ki te aukati i te pokanoa: karekau he pokanoa i te wa i whakamatauria ai nga matū whakararuraru ki enei kohinga e whai ake nei: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) toto tangata, levofloxacin (10μg/mL), moxifloxacin(0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin(125μg/mL), erythromycin(0.5g/L), doxycycline (50mg /L), minocycline (0.1g/L). |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu (Hongshi Medical Technology Co., Ltd.) Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu (FQD-96A, Hangarau Hangzhou Bioer) MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
(1) Tauira Sputum
Reagent tangohanga Manakohia: Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Tonotono & Micro-Test Tango Waikawa Nucleic Aunoa (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Taapirihia te 200µL o te tote noa ki te parapara kua mahia.Ko te tangohanga i muri mai me mahi kia rite ki nga tohutohu mo te whakamahi.Ko te 80µL te rōrahi elution e taunakitia ana. Reagent tangohanga e taunakitia ana: Tangohanga Acid Nucleic, Reagent Purification ranei (YDP315-R).Ko te tangohanga me tino rite ki nga tohutohu mo te whakamahi.Ko te rōrahi hauhā e tūtohu ana ko te 60µL.
(2) Oropharyngeal swab
Reagent tangohanga Manakohia: Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Tonotono & Micro-Test Tango Waikawa Nucleic Aunoa (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me mahi te tangohanga kia rite ki nga tohutohu mo te whakamahi.Ko te rahinga tangohanga o te tauira ko te 200µL, a ko te 80µL te rōrahi elution e taunakitia ana. Reagent tangohanga e taunakitia ana: QIAamp Viral RNA Mini Kit (52904) or Nucleic Acid Extraction or Purification Reagent (YDP315-R).Ko te tangohanga me tino rite ki nga tohutohu mo te whakamahi.Ko te 140µL te rōrahi tangohanga o te tauira, me te 60µL te rōrahi hauhā.
