Mahi Tino Pai & Whakamahinga Whānui
Ngāwari te Whakakaha, mā te hangarau o ite whakanui waikawa karihi sothermalhe mea whakaatu ki te teiteite aro me tepototauhohengawā kāore he whakaritenga mō tukanga whakarerekētanga pāmahana. Nō reira, kei a iaputa maiedhei tete nuingate hangarau pai mō te whakamātautau tere o te waikawa karihi.Kāore he uauatangangā pūnaha huringa hermal, he taputapu whakanui isothermal māmā, kawe hokika taea te whakamātau i tete tukanga tauhohenga katoa.
Kua whakawhanakehia motuhaketia e te Macro & Micro-Testā, i whakarewahiate EasyAmp,he taputapu whakanui isothermal fluorescent kawemō ngā momo tono.
I tēnei wā, ko teWhakakaha Ngāwari kua whakamanahia kiahototahi ki ētahi tikanga whakanui isothermal: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), me Helicase Dependent Amplification (HDA)He taputapu hou anō tēnei mō te whakamātautau tere i te waikawa karihi, ā, he whānui ake ngā tono.
· Whakareitanga Isothermal Whakawhitiwhiti-Aroturuki (RAMA):Whakarei ake i60-65°Cme te katoatauhohengatukangaoti i roto i te 30 meneti.Tirohia te Pikitia 1 mō tōna whakanuikaupapa:
Pikitia 1. Mātāponoof RAMA
· Te Whakarei i te Recombinase Polymerase (RPA/RAA/MIRA):Whakarei ake i37°C-42°Cme te katoatauhohengatukangaoti i roto i te 10-30 meneti.Tirohia te Pikitia 2 mō tōnate kaupapa whakanui:
Pikitia 2. Mātāponoof Whakarei ake i te Recombinase Polymerase
·Te Whakarei i te Raupapa Waikawa Karihi (NASBA):Whakarei ake i42°Cme te katoatauhohengatukangai oti i roto i te 40-120 meneti.Tirohia te Pikitia 3 mō tōnate kaupapa whakanui:
Pikitia 3. Mātāponoof Whakarei ake i te raupapatanga waikawa karihi
· Whakareitanga Whakawhirinaki ki te Helicase (HDA):Whakarei ake i65°Cme te katoatauhohengatukangai oti i roto i te 70-90 meneti.Tirohia te Pikitia 4 mō tōnate kaupapa whakanui:
Pikitia 4. Mātāponoof Whakareitanga e whakawhirinaki ana ki te Helicase
· Te EasyAmpwhakahoheHe whakakaha whai hua, pumau hoki mō te kimi i te DNA me te RNA mā te whakamahi i ngā tikanga isothermal e whā kua whakahuatia ake nei. Ka tutuki i a ia te aro ki te whakakaha tae atu ki te 200 tārua/mL, ā, ka whakarite i te tino motuhake me te kore he tauhohenga whakawhiti i roto i te whakakaha ūnga..(I(e whakaaturia ana i roto i ngā Pikitia 5 ki te 7).
Te Kimi i te DNA Te Kimi i te RNA
Pikitia 5. Pūhara RAA/MIRA
Te Kimi i te DNA Te Kimi i te RNA
Pikitia 6. Papa RĀMA
Te Kimi i te DNA Te Kimi i te RNA
Pikitia 7. Pūhara NASBA
Ngā Painga o te Hua:
Tere
· EPIAka mōhio te hangarauhua paii kitea i roto i te 5 meneti.
· He ngāwari te whakahaere me te tango kotahi-taahiraamō te whakanui i runga i te taputapu.
Whai hua
· 4 ngā kōwae motuhake mō te tono-ā-ringa, te whakamātautau i te wā kotahi o ngā momo rerekē e 4momo whakamātautaus.
· Ka taea e ia kōwae te tātari i ngā tauira e whā i te wā kotahi.
· Takirua/Takiwhāfte kanapangā hongeremō te kimi whānui i ngā ūnga maha.
Hototahitanga
· Hototahi kimahangā papaaho isothermal pēnei i te LAMP, RPA, NASBA, HDA.
· I hangaia mō te whakanui pāmahana teitei i muri i te tuhi whakamuri pāmahana iti.
· Whakariteaāheihei whakatutuki i ngā hiahia motuhake a te kiritaki.
He ngāwari ki te whakamahi
· Phoahoa papaaho(290×245×128mm, 2kg) mō ngā momo kaupapa tono.
· Mata pa tae 7-inihimō te whakahaere ngāwari, me te whakaaturanga hua oraora.
· He pūreretā wera ā-roto mō te tānga tere tonu.
· Matawai whakauru mō te pānui aunoa i ngā tūemi.
Wā tuku: Hakihea-22-2023