Mahinga Pai me te Whakamahinga Whanui
Easy Amp, na te hangarau o ite whakanui i te waikawa nucleic sothermale whakaatuhia ana me te teiteitairongo mepototauhohengawā kahore he whakaritenga mo tukanga huringa pāmahana. No reira, kei a iaputaedhei tete nuingahangarau pai mo te whakamatautau waikawa karihi tere.Te kore uaua tnga punaha paihikara hermal, he taputapu whakakaha hau werawera ngawari me te kaweka taea te makona i tetukanga tauhohenga katoa.
Ko te Tonotono me te Moroiti-Whakamātautau kua whanake takitahika whakarewahiate EasyAmp,he taputapu whakakaha hau werawera kawemo nga momo tono.
I tenei wa, ko teAmp ngawari kua whakamanahia heihe hototahi ki te maha o nga tikanga whakakaha hauwaariki: Roop-Mediated Isothermal Amplification(LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification(NASBA), me Helicase Dependent Amplification(HDA).Ko tetahi atu taputapu tapahi mo te whakamatautau waikawa karihi tere me nga tono whanui ake.
· Koropiko-Mediated Isothermal Amplification (LAMP):Whakanuia i60-65°Cme te katoatauhohengatukangaka oti i roto i te 30 meneti.Ka kite i te Whakaahua 1 mo tona whakakahaparau tumu:
Fig 1. Tikangaof RAMA
· Recombinase Polymerase Amplification (RPA/RAA/MIRA):Whakanuia i37°C-42°Cme te katoatauhohengatukangaka oti i roto i te 10-30 meneti.Ka kite i te Whakaahua 2 mo onate kaupapa whakakaha:
Fig 2. Tikangaof Recombinase Polymerase Amplification
·Whakanuia i runga i te Raupapa Waikawa Nucleic (NASBA):Whakanuia i42°Cme te katoatauhohengatukangaka oti i roto i te 40-120 meneti.Ka kite i te Whakaahua 3 mo onate kaupapa whakakaha:
Fig 3. Tikangaof Te Whakanuia i te Raupapa Waikawa Nucleic
· Ko te Whakanui-a-Helicase (HDA):Whakanuia i65°Cme te katoatauhohengatukangaka oti i roto i te 70-90 meneti.Ka kite i te Whakaahua 4 mo onate kaupapa whakakaha:
Fig4.Tikangaof Ko te Whakanuia Tiaki Helicase
· Te EasyAmptaeas te kaha me te kaha o te whakakaha mo te rapunga DNA me te RNA ma te whakamahi i nga tikanga weariki e wha kua kiia ake nei.Ka tutuki i te kaha whakakaha ake ki te 200 kape/mL me te whakarite i te tino motuhake me te kore he tauhohenga whakawhiti i roto i te whakanui i te whaainga..(Ie whakaatuhia ana i nga Whakaahua 5 ki te 7).
Te Tirohanga DNA Te Tirohanga RNA
Fig5.RAA/MIRA Platform
Te Tirohanga DNA Te Tirohanga RNA
Fig6.RAMA Paerewa
Te Tirohanga DNA Te Tirohanga RNA
Fig7.Paerewa NASBA
Hua Painga:
tere
· EPIAmohio hangarauhua paikitea i roto i te 5 min.
· He mahinga ngawari me te tangohanga kotahi-taahiraamo te whakauru i runga taputapu.
He pai
· E 4 nga waahanga motuhake mo te tono, whakamatautau kotahi mo te 4 rerekemomo whakamatautaus.
· Ka taea e ia kōwae te wetewete i nga tauira e wha i te wa kotahi.
· Tārua/Tawhāfwhakahihirihongeremo te rapunga-maha-whanui.
Hototahi
· Hototahi kimahatüäpapa hauwaro përä i te LAMP, RPA, NASBA, HDA.
· I hoahoatia mo te whakamaaramatanga teitei o te pāmahana i muri i te whakamaoritanga whakamuri o te pāmahana iti.
· Whakariteataeaki te whakatutuki i nga hiahia o te kiritaki.
Kaiwhakamahi-hoa
· Phoahoa ortable(290×245×128mm, 2kg) mo momo senarios tono.
· 7-inihi mata pa taemo te ngawari o te whakahaere me te whakaatu hua marama.
· He pūreretā waiariki i hangaia mo te tānga hua tonu.
· Matawai whakauru mo te panui tuemi kitea aunoa.
Wā tuku: Tihema-22-2023