—— He Rangahau Ngātahi nā Zhejiang CDC, Macro & Micro-Test, me Haina CDC I whakaputaina ki Frontiers in Cellular and Infection Microbiology
Tirohanga Whānui o te Akoranga
I te marama o Mei 2026, i whakaputa a Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) i tētahi pepa i arahina e te Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), me te tīma bioinformatics mai i Beijing Macro & Micro-Test Bio-Tech Co., Ltd. me te National Institute for Communicable Disease Control and Prevention (China CDC) hei kaituhi tahi. Ko te taitara o te rangahau ko:
"Te tautuhi me te tātaritanga phylogenetic o ngā momo Brucella abortus e whitu i Zhejiang, Haina."
Ko tēnei rangahau te tātaritanga pūnaha tuatahi mō te whai i te Brucella abortus (B. abortus) e ahu mai ana i te ira katoa i Haina. I tātarihia e te tīma e whitu ngā momo i kohia mai i te tau 2015 ki te 2025 (e whā ngā momo i ahu mai i te tangata me ētahi atu momo i ahu mai i te kau mai i Jinhua, Quzhou, me Ningbo). Mā ngā kitenga ka kitea he taunakitanga ira mō te takenga mai me ngā ara tuku o tēnei "momo matua ki te raki" i tētahi rohe mate urutā ki te tonga kāore i te tino kitea i te rawhiti o Haina.
Papamuri me te Hiranga
He mate kararehe te Brucellosis i puta mai i ngā huakita o te puninga Brucella. Ko te Brucella abortus te mate nui ki ngā kau, engari ka taea hoki e ia te mate i roto i te tangata. I Haina, he rerekētanga ā-whenua te brucellosis: ko te nuinga o te mate ka puta i ngā kawanatanga o te raki (hei tauira, Inner Mongolia, Shanxi, Heilongjiang). He rerekē, ko ngā kawanatanga o te tonga, tae atu ki a Zhejiang, kua roa e noho ana te Brucella melitensis, ā, he iti noa iho ngā kēhi kua pūrongohia mō te B. abortus. Nā tēnei rerekētanga ā-rohe, ko te whakaahuatanga ira me te rapunga pūtake o te B. abortus i Zhejiang te kaupapa matua mō te hauora tūmatanui.
Ngā Tikanga me ngā Kitenga Matua
I whakamahia e te tīma rangahau tētahi rautaki maha-mātāpuna e whakakotahi ana i te koiora ngota me te koiora-pārongo:
1.Te tautuhi i te mate urutā me te momo taketake
I whakaūtia e te PCR ira BCSP-31 me te AMOS-PCR ko ngā momo ira e whitu he B. abortus.
I whakaatuhia e te momo raupapatanga maha-locus (MLST) i runga i ngā ira e iwa e pupuri ana i ngā ira, nō te momo raupapatanga ST2 ngā momo katoa, e tohu ana i te ōritetanga ira teitei i waenga i ngā momo B. abortus e horapa ana i Zhejiang.
2.Te tautuhi i te ira katoa
I whakahaerehia te raupapatanga ira-katoa i runga i te tūāpapa Illumina NovaSeq. I whakaatuhia e te tātaritanga tuakiri nucleotide toharite (ANI) he rite te āhua o ngā momo Zhejiang ki te momo tohutoro B. abortus 544 tae atu ki te 99.99%.
I whakaatuhia e te tātaritanga whānui-ira he taupori tino tiakina: 3,084 ngā ira matua i tautuhia, me ngā ira anga 10 anake, ā, kāore i kitea he ira matua ngohengohe, he ira kapua rānei.
3.Ngā kōtaha ira o te kaha ki te wero me te ātete ki ngā huakita
E 68 katoa ngā āhuatanga e pā ana ki te kaha o te mate i matapaetia, e kapi ana i ngā ara tawhito pērā i te hanga LPS, te pūnaha huna T4SS, me te pūnaha whakahaere wāhanga-rua o te BvrR-BvrS. He mea nui, kāore i roto i ngā momo katoa ngā ira piri bmaA me btaF. I kitea e te tātari ira ātete te ira mprF anake i roto i te pātengi raraunga CARD, kāore hoki i kitea ētahi atu āhuatanga ātete.
4. Te hanga anō i te hanganga ira me te whai i te tuku
Nā te tātaritanga o te ira matua kotahi-nucleotide polymorphism (cgSNP) i whakanoho ngā momo Zhejiang ki tētahi tūranga motuhake i roto i te rākau whakawhanaungatanga ā-ao. I whakaatuhia e ngā hua he rōpū monophyletic ngā momo Zhejiang me ngā momo mai i Rūhia, Mongolia, me ētahi kawanatanga o te raki o Haina (Ningxia, Heilongjiang, Mongolia o Roto, Hebei, Gansu, Beijing). Ka wehea anō tēnei rōpū ki ngā momo iti motuhake e toru (Momo 1–3), e tohu ana i ngā huihuinga whakauru motuhake maha.
Ngā Whakatau me ngā Pānga
Nā tēnei rangahau i whakarato te huinga raraunga ira tuatahi tino tika o B. abortus i te Porowini o Zhejiang, ā, e hua ake ana ētahi whakatau matua:
- Clear papamuri ira– Nō te ST2 ngā momo B. abortus e horapa ana i Zhejiang, ā, he tino tiakina te ira, ā, he momo whakapapa noa iho nō te brucellosis kau.
2. Evite kaha o te tuku whakawhiti-rohe– Kāore te tātaritanga phylogenetic e tautoko i te noho o tētahi whakapapa taketake motuhake i Zhejiang. Engari, e kī ana ngā raraunga i ahu mai ēnei momo i te raki o Haina, ā, tera pea he rite tonu te papamuri whanaketanga ki ngā momo i Rūhia me Mongolia. Ko te noho mai o ngā momo iti e toru e tohu ana i ngā huihuinga whakaurunga maha.
3. Ngā pānga ki te hauora tūmatanui– E whakaatu ana ngā kitenga i te hiranga o te tirotiro ira mō te brucellosis ahakoa i ngā rohe kāore i te noho tahi i te nuinga o te wā pērā i a Zhejiang. Ahakoa he iti te maha o ngā keehi o nāianei, ka taea e ngā taputapu whakatau teitei pērā i te cgSNP te whai i te pūtake o ngā pakarutanga kawemai me te whakarato taunakitanga pūtaiao hei whakakore i ngā mekameka tuku e pā ana ki te kawe kararehe i waenga i ngā rohe.
Ehara i te mea ka whakakīia e tēnei mahi tētahi āputa rangahau i te Porowini o Zhejiang anake, engari ka whakaratohia hoki he raraunga tūāpapa hou mō te tirotiro i ngā mate urutā me te aromatawai i te mōrearea o te brucellosis i te rohe o te awa o Yangtze.
Ngā Mōhiohio Pepa:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Te tautuhi me te tātaritanga phylogenetic o ngā momo Brucella abortus e whitu i Zhejiang, Haina. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Wā tuku: Pipiri-10-2026