15 Ngā Momo Ira Tangata Papillomavirus E6/E7 mōrearea-nui mRNA
Ingoa hua
HWTS-CC005A-15 Ngā Momo Kete Kimi Ira MRNA Papillomavirus Tangata E6/E7 mōrearea nui (PCR Fluorescence)
Te mate urutā
Ko te mate pukupuku o te kōpū tetahi o ngā momo mate pukupuku wahine e tino kitea ana puta noa i te ao, ā, he hononga tata tōna putanga mai ki ngā huaketo papilloma tangata (HPV), engari he iti noa iho te wāhanga o ngā mate HPV ka taea te whanake hei mate pukupuku. Ka pangia e te HPV mōrearea nui ngā pūtau epithelial o te kōpū, ā, ka puta mai ngā oncoprotein e rua, arā, ko E6 me E7. Ka taea e tēnei pūmua te pā ki ngā momo pūmua pūtau (pēnei i ngā pūmua pehi pukupuku pRB me p53), ka whakaroa i te huringa pūtau, ka pā ki te hanganga DNA me te pumau o te ira, ā, ka whakararuraru i ngā urupare ārai mate huaketo me te ārai pukupuku.
Hongere
| Hongere | Wāhanga | I whakamatautauria te momoira |
| FAM | Pūnaha Tauhohenga HPV 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Ira β-actin tangata | |
| FAM | Pūnaha Tauhohenga HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Ira INS tangata |
Ngā Tawhā Hangarau
| Rokiroki | Wai: ≤-18℃ |
| Te roa o te oranga | 9 marama |
| Momo Tauira | Te ūkui o te kaki |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me whakahaere te tango kia rite tonu ki ngā tohutohu whakamahi. Ko te rōrahi wairewa e taunakitia ana ko te 50μL. Mena kāore te tauira i tino nakunaku, whakahokia ki te taahiraa 4 hei nakunaku anō. Kātahi ka whakamātauria kia rite ki ngā tohutohu whakamahi.
Te matū tango e taunakitia ana: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Me whakahaere te tango kia rite ki ngā tohutohu whakamahi (I te taahiraa 5, whakareatia te kukū o te otinga mahi DNaseI, arā, tangohia te 20μL o te otinga rakau RNase-Free DNaseI (1500U) ki roto i tētahi ngongo centrifuge RNase-Free hou, tāpirihia te 60μL o te RDD buffer, ka whakaoho mārie). Ko te rōrahi wairewa e taunakitia ana ko te 60μL. Mena kāore te tauira i tino nakunaku, whakahokia ki te taahiraa 5 kia nakunaku anō. Kātahi ka whakamātauria kia rite ki ngā tohutohu whakamahi.
