15 nga momo o te tangata-nui rawa atu o te tangata papillomavirus e6 / e7 gener mrna
Ingoa Hua
HWSS-CC005A-15 nga momo o te tangata-nui rawa o te papillillomavirus e6 / E7 ira tohu mot (florescence pcr)
Manganga
Ko te mate pukupuku cervical tetahi o nga momo mate pukupuku o te ao, ko tona mahinga ka piri ki te papillomavirus tangata (HPV), engari he iti noa iho te mate o te mate HPV ka taea e te mate pukupuku. Ko te HPV tino tupono kei te mate ka mate nga pūtau epithelial cervical ka whakaputa e rua oncoproteins, E6 me E7. Ka taea e tenei pūmua te pa ki te maha o nga pūtau pūtau (pēnei i te huringa o te puku o te puku, ka pa ki te huringa DNA.
Takiwā rerenga
| Takiwā rerenga | Wāhanga | I whakamatauria a Genotype |
| Whānau | HPV tauhohenga HPV 1 | HPV16,31,33,35,51,52,58 |
| Vic / Hex | Tangata β-Actin Gene | |
| Whānau | HPV tauhohenga HPV 2 | HPV 18,39,45,53,53,59,66,66,68 |
| Vic / Hex | Ira tangata |
Tohu hangarau
| Pākoro | Wai: ≤-18 ℃ |
| Te Haahi-Ora | 9 marama |
| Momo tauira | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| Koropū | 500 kape / ml |
| Nga taputapu e tika ana | Te tono Biosystems 7500 Pūnaha PCR-RealTe tono Biosystems 7500 nga punaha PCR-tino-tino tere Raupaparorohiko PCR-RealTudio®5 Slan-96P Pūnaha PCR tūturu Pūnaha PCR Realcycler®480 Ko te Pūnaha Tango-Real PCR Ma-6000 Motuhake TheMal Thermal Thermal Biorad CFX96 Pūnaha Real-Time Biorad CFX Opus 96 Pūnaha Real-Time |
Rere mahi
Whakahoahoa Whakawhiwhinga Tauhokohoko: Macro & Kit-Roro-Whakamātautau Virat (HWS-3020-50-HPV15) na Jiangsu Macro & Midd-Add-Tech CA., Ltd. Ko te tangohanga kia rite ki nga tohutohu mo te whakamahi . Ko te rōrahi whakahiatotanga ko te 50μl. Mena kaore e tino keri te tauira, whakahokia mai ki te taahiraa 4 mo te whakatikatika. Katahi ka whakamatau kia rite ki nga tohutohu mo te whakamahi.
Whakawhiwhinga Tango: rnaprep parakore te kiko kararehe matū rna e tango ana i te kete tangohanga (DP431). Me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi i te tino mahi (i te kaha o te otinga mahi DNASEI, ara, 20μL) te otinga a Rnase-Free Dnase-Free ki roto i te Tube Rnase-koreutu. Tāpirihia te 60μl o Rdd Buffer, ka uru ki te ata). Ko te rōrahi whakahiatotanga e 60μl. Mena kaore e tino keri te tauira, whakahokia mai ki te taahiraa 5 mo te whakatikatika. Katahi ka whakamatau kia rite ki nga tohutohu mo te whakamahi.
