28 Ngā Momo o te Huaketo Papilloma Tangata Morearea-Tiketike (16/18 Momo) Waikawa Nucleic
Ingoa hua
HWTS-CC006A-28 Ngā Momo Huaketo Papilloma Tangata Morearea-Tiketike (16/18 Momo) Kete Kimi Waikawa Karihi (PCR Fluorescence)
Te mate urutā
Ko te mate pukupuku o te kōpū tetahi o ngā pukupuku kino tino kitea o te ara whakaputa uri wahine. Kua whakaatuhia e ngā rangahau ko ngā mate HPV pumau me ngā mate maha tetahi o ngā tino take o te mate pukupuku o te kōpū. I tēnei wā, kāore anō kia kitea he maimoatanga whai hua mō te mate pukupuku o te kōpū i puta mai i te HPV, nō reira ko te kitenga wawe me te aukati i te mate pukupuku o te kōpū i puta mai i te HPV te mea nui ki te aukati i te mate pukupuku o te kōpū. He mea nui te whakatū i tētahi whakamātautau tātaritanga take māmā, motuhake me te tere mō te tātaritanga haumanu me te maimoatanga o te mate pukupuku o te kōpū.
Hongere
| Ranunga Tauhohenga | Hongere | Momo |
| PCR-Whakaranu1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Mana Whakahaere ā-roto | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | Wai: ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Te ūkui waha, te ūkui tara, te mimi |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tāpirihia he 200μL o te tote noa hei whakapūmau anō i te pire i te taahiraa 2.1, kātahi ka whakahaerehia te tangohanga kia rite ki ngā tohutohu mō te whakamahinga o tēnei matū tangohanga. Ko te rōrahi wairewa e taunakitia ana ko te 80μL.
Te matū tango e taunakitia ana: QIAamp DNA Mini Kit (51304) te Macro & Micro-Test Viral DNA/RNA Column rānei (HWTS-3020-50). Tāpirihia he 200μL o te tote noa hei whakapūmau anō i te pire i te taahiraa 2.1, kātahi ka whakahaerehia te tangohanga kia rite ki ngā tohutohu mō te whakamahinga o tēnei matū tangohanga. Ko te rahinga tauira i tangohia o ngā tauira he 200μL katoa, ā, ko te rahinga wairewa e taunakitia ana he 100μL.
