28 Momo o te Human Papilloma Virus (16/18 Pato) Waikawa Nucleic
ingoa hua
HWTS-CC006A-28 Nga Momo Waoronga Papilloma Tangata (16/18 Pato) Kete Rapu Waikawa Nucleic (Fluorescence PCR)
Epidemiology
Ko te mate pukupuku servikal tetahi o nga pukupuku kino e tino kitea ana i roto i te waahanga whanau wahine. Kua whakaatuhia e nga rangahau ko nga mate HPV mau tonu me nga mate maha tetahi o nga tino take o te mate pukupuku pukupuku. I tenei wa, kei te ngaro tonu nga rongoa whai hua mo te mate pukupuku pukupuku na te HPV, no reira ko te kitenga wawe me te aukati i te mate pukupuku na te HPV te mea matua hei aukati i te mate pukupuku pukupuku. He mea tino nui ki te whakarite i tetahi whakamatautau tātari etiology ngawari, motuhake me te tere mo te taatai haumanu me te maimoatanga o te mate pukupuku pukupuku.
Hongere
| Tauhohenga Mix | Hongere | Momo |
| PCR-Ranu1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Mana o roto | |
| PCR-Ranu2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Mana o roto |
Tawhā Hangarau
| Rokiroki | Wai: ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te Mimi, Te Wahine Wahine, Te Mimi |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300Topi/mL |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent tangohanga Manakohia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor-300 HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Taapirihia te 200μL o te tote noa hei whakamutu i te pire i te taahiraa 2.1, katahi ka whakahaerehia te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te 80μL te rōrahi elution tūtohu.
Reagent tangohanga Manakohia: QIAamp DNA Mini Kit (51304) or Tonotono & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Taapirihia te 200μL o te tote noa ki te whakamutu i te pire i te taahiraa 2.1, katahi ka whakahaerehia te tangohanga kia rite ki nga tohutohu mo te whakamahi i tenei reagent tangohanga. Ko te 200μL te rahinga tauira kua tangohia, ko te 100μL te rōrahi elution e taunaki ana.
