Enterovirus Universal
ingoa hua
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ko te mate ringa-waewae-waha he mate hopuhopu na te enteroviruses (EV).I tenei wa, 108 nga momo serotypes o enteroviruses kua kitea, ka wehewehea ki nga roopu e wha: A, B, C me D. I roto i a raatau, ko te enterovirus EV71 me te CoxA16 nga pathogens matua.Ko te nuinga o te mate ka pa ki nga tamariki kei raro iho i te 5 tau te pakeke, ka pa te mate herpes ki nga ringa, waewae, waha me etahi atu waahanga.He tokoiti o nga tamariki ka puta nga raruraru penei i te myocarditis, te edema pulmonary, me te maningoencephalitis aseptic.
Hongere
| FAM | EV RNA |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | ūkui oropharyngealHerpes wai |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Kape/mL |
| Nga taputapu e tika ana | Hangaia nga Pūnaha koiora 7500/7500 Pūnaha PCR Wā-tūturu Tere, QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Tūtohu Kete Tangohanga: Tohutono & Mokoiti-Whakamātautau General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Tonotono & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), me tino tangohia kia rite ki nga tohutohu.Ko te rahinga tauira ko te 200 μL, ko te 80µL te rōrahi elution e taunaki ana.
Kōwhiringa 2.
Ko te kete tangohanga e taunakihia ana: Tonotono me te Micro-Test Sample Release Reagent (HWTS-3005-8), me tino tangohia kia rite ki nga tohutohu.
Kōwhiringa3.
Ko te kete tangohanga e manakohia ana: QIAamp Viral RNA Mini Kit (52904) or Nucleic Acid Extraction or Purification Kit (YDP315-R), me tango kia rite ki nga tohutohu.Ko te rahinga tauira he 140 μL, ko te rōrahi elution e taunaki ana ko te 60μL.
