Enterovirus Ao Whānui
Ingoa hua
HWTS-EV001- Kete Kimi Waikawa Karihi Ao Whānui mō te Enterovirus (PCR Fluorescence)
Te mate urutā
Ko te mate ringa-waewae-waha he mate hopuhopu i puta mai i ngā enterovirus (EV). I tēnei wā, e 108 ngā momo serotype o ngā enterovirus kua kitea, e wehea ana ki ngā rōpū e whā: A, B, C me D. I roto i ēnei, ko te enterovirus EV71 me te CoxA16 ngā mate matua. Ko te nuinga o te mate ka pā ki ngā tamariki kei raro iho i te 5 tau te pakeke, ā, ka taea e ia te whakaputa i te herpes ki ngā ringa, waewae, waha me ētahi atu wāhanga. Ka pāngia he iti noa ngā tamariki e ngā raruraru pēnei i te myocarditis, te pulmonary edema, me te aseptic meningoencephalitis.
Hongere
| FAM | RNA EV |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | He ūkui waha,Wai herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/mL |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha PCR Tere Wā-Tūturu o Applied Biosystems 7500/7500, QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Kete Tangohanga e taunakitia ana: Kete Whakamātautau-Makro me te Iti-Whakamātautau Whānui DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) me te Macro & Micro-Whakamātautau Aunoa Waikawa Nucleic (HWTS-3006B, HWTS-3006C), me tango kia rite ki ngā tohutohu. Ko te rahinga tauira he 200 μL, ko te rahinga wairewa e taunakitia ana he 80µL.
Kōwhiringa 2.
Kete tango e taunakitia ana: Macro & Micro-Test Sample Release Agent (HWTS-3005-8), me tango kia rite ki ngā tohutohu.
Kōwhiringa3.
Kete tango e taunakitia ana: QIAamp Viral RNA Mini Kit (52904) Nucleic Acid Extraction, Purification Kit rānei (YDP315-R), me tango kia rite tonu ki ngā tohutohu. Ko te rahinga tauira he 140 μL, ko te rahinga wairewa e taunakitia ana he 60µL.
