Staphylococcus Aureus me te Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

Whakaahuatanga Poto:

Ka whakamahia tenei kete mo te rapunga tohu o te staphylococcus aureus me te methicillin-resistant staphylococcus aureus waikawa nucleic i roto i nga tauira sputum tangata, tauira pania ihu me nga tauira mate kiri me te kiko ngohengohe in vitro.


Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-OT062 Staphylococcus Aureus me te Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Kete Rapu Waikawa Nucleic(Fluorescence PCR)

Tiwhikete

CE

Epidemiology

Ko Staphylococcus aureus tetahi o nga huakita pathogenic nui o te mate nosocomial.Ko te Staphylococcus aureus (SA) no te staphylococcus, he kanohi mo nga huakita Gram-positive, ka taea te whakaputa i nga momo paitini me nga whākōkī urutomo.Ko nga huakita nga ahuatanga o te tohatoha whanui, te kaha o te pathogenicity me te tere o te aukati.Ko te ira karihi Thermotable (nuc) he ira tino tiakina o staphylococcus aureus.

Hongere

FAM ira mecA ātete-methicillin
ROX

Mana o roto

CY5 staphylococcus aureus ira nuc

Tawhā Hangarau

Rokiroki ≤-18 ℃ & parea i te marama
Papa-ora 12 marama
Momo Tauira te huhu, nga tauira mate kiri me te kiko ngohe, me nga tauira pania ihu
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL huakita ātete-methicillin.Ina kitea e te kete te tohutoro LoD a-motu, ka kitea te 1000/mL staphylococcus aureus.
Tauwhāiti E whakaatu ana te whakamatautau tauhohenga whakawhiti karekau he tauhohenga whiti o tenei kete ki etahi atu tukumate manawa penei i te methicillin-tairongo staphylococcus aureus, coagulase-kino staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniellaprotete mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Nga taputapu e tika ana Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu

QuantStudio®5 Pūnaha PCR Wā-tūturu

SLAN-96P Pūnaha PCR Wā-tūturu

Kaihurihuri®480 Pūnaha PCR Wā-tūturu

LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu

MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha

BioRad CFX96 Pūnaha PCR Wā-tūturu

BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu

Rere Mahi

Kōwhiringa 1.

Tonotono & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Taapirihia te 200µL o te tote noa ki te parapara kua oti te tukatuka, me te tango i nga mahi ka whai ake i runga i nga tohutohu, a ko te 80µL te rōrahi elution e taunaki ana.

Kōwhiringa 2.

Tonotono & Micro-Test Tauira Tuku Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Taapirihia te 1mL o te tote noa ki te para i muri i te horoinga ki te tote noa, ka uru pai.Whakanuia ki te 13,000r/min mo te 5 meneti, tangohia te supernatant (rahui 10-20µL o te supernatant), ka whai i nga tohutohu mo te tangohanga o muri.

Reagent tangohanga Manakohia: Tangohanga Acid Nucleic or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd.Ko te tangohanga me tino rite ki te taahiraa 2 o te pukapuka tohutohu.E taunaki ana kia whakamahia te wai RNase me te DNase-kore mo te whakamaarama me te rahi o te 100µL.

 


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