Te Waikawa Matū o Zaire me Sudan Ebolavirus kua maroke-tio
Ingoa hua
HWTS-FE035-Kete Kimi Waikawa Matūriki o Zaire me Sudan Ebolavirus (PCR Fluorescence) kua maroke-tio
Te mate urutā
Nō Filoviridae te huaketo Ebola, he huaketo RNA aho-kotahi kāore i te wehewehea. He miro roa ngā huaketo, ko te roa toharite o te virion he 1000nm, ā, ko te whānui he 100nm pea. Ko te ira Ebola he RNA aho-kotahi kāore i te wehewehea, ko te rahi he 18.9kb, e whakamārama ana i ngā pūmua hanganga e 7 me te pūmua kore-hanganga kotahi. Ka taea te wehewehe i te huaketo Ebola ki ngā momo pēnei i a Zaire, Sudan, Bundibugyo, Tai Forest me Reston. I roto i ēnei, kua pūrongohia ko te momo Zaire me te momo Sudan te take o te mate o te tini tāngata. Ko te EHF (Ebola Hemorrhagic Fever) he mate pāwera toto nui i puta mai i te huaketo Ebola. Ko te nuinga o te tangata ka pangia e te pā atu ki ngā wai tinana, ngā mea huna me ngā paru o ngā tūroro, o ngā kararehe pangia rānei, ā, ko ngā whakaaturanga haumanu ko te kirika e puta mai ana, te toto me te kino o ngā okana maha. He teitei te reiti mate o te EHF, 50%-90%. I tēnei wā, ko ngā tikanga mō te tātaritanga o te huaketo Ebola he whakamātautau taiwhanga te nuinga, tae atu ki ngā āhuatanga e rua: te kimi pūtake me te kimi serological. Ko te kimi pūtake ko te kimi i ngā antigens huaketo i roto i ngā tauira toto mā te ELISA, te kimi i ngā waikawa karihi mā ngā tikanga whakanui pēnei i te RT-PCR, me ētahi atu, me te whakamahi i ngā pūtau Vero, Hela, me ētahi atu hei wehe me te whakatipu huaketo. Ko te kimi serological ko te kimi i ngā paturopi IgM motuhake i roto i te toto mā te hopu ELISA, me te kimi i ngā paturopi IgG motuhake i roto i te toto mā te ELISA, immunofluorescence, me ētahi atu.
Ngā Tawhā Hangarau
| Rokiroki | ≤30℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | serum, plangā tauira sma |
| CV | ≤5.0% |
| LoD | 500 Ngā Tārua/μL |
| Ngā Taputapu e Pā Ana | E pā ana ki te matū kimi momo I: Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu, QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu, Ngā Pūnaha PCR Wā-Tūturu SLAN-96P (Hongshi Medical Technology Co., Ltd.), Ngā Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer), MA-6000 Pūrere Huringa Wera Tau-Tūturu (Suzhou Molarray Co., Ltd.), Pūnaha PCR Wā-Tūturu BioRad CFX96, Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96. E pā ana ki te matū kimi momo II: ŪtemonaTMAIO800 (HWTS-EQ007) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Rere Mahi
Kete Whakamātautau-Macro & Micro-Test Viral DNA/RNA (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Me whakahaere te tangohanga kia rite ki ngā tohutohu, ā, ko te rōrahi tauira i tangohia he 200μL, ā, ko te rōrahi wairewa i tūtohutia he 80μL.
