Te Whakapūtanga Ira Tangata CYP2C9 me VKORC1
Ingoa hua
Kete Kimi i te HWTS-GE014A-CYP2C9 me te VKORC1 Ira Tangata (PCR Fluorescence)
Tiwhikete
CE/TFDA
Te mate urutā
Ko te Warfarin he rongoā ārai toto ā-waha e whakamahia whānuitia ana i roto i ngā mahi haumanu i ēnei rā, ā, ko te nuinga o te wā he ārai me te rongoā i ngā mate thromboembolic. Heoi, he iti noa te wā maimoatanga o te warfarin, ā, he rerekē rawa i waenga i ngā iwi me ngā tāngata takitahi. E ai ki ngā tatauranga, neke atu i te 20 ngā wā ka rerekē te horopeta pumau i roto i ngā tāngata takitahi. Ka puta te toto kino i roto i te 15.2% o ngā tūroro e tango ana i te warfarin i ia tau, ā, 3.5% o ēnei ka puta he toto whakamate. Kua whakaatuhia e ngā rangahau pharmacogenomic ko te polymorphism ira o te whākōkī ūnga VKORC1 me te whākōkī metabolic CYP2C9 o te warfarin he mea nui e pā ana ki te rerekētanga o te horopeta o te warfarin. Ko te Warfarin he aukati motuhake o te huaora K epoxide reductase (VKORC1), ā, nā reira ka aukati i te hanganga o te ira toto e uru ana ki te huaora K, ā, ka whakarato i te anticoagulation. He maha ngā rangahau kua tohu ko te polymorphism ira o te kaiwhakatairanga VKORC1 te mea nui e pā ana ki te iwi me ngā rerekētanga takitahi o te horopeta warfarin e hiahiatia ana. Ka whakapūmautia te warfarin e CYP2C9, ā, ka tino puhoi ake te pākia o te warfarin e ōna matū whakarerekētanga. He nui ake te mōrearea (e rua ki te toru ngā wā) o te toto i te hunga e whakamahi ana i te warfarin i te wā tōmua o te whakamahinga.
Hongere
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Ngā Tawhā Hangarau
| Rokiroki | Wai: ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Toto hou kua āraihia te toto EDTA |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Te motuhaketanga | Kāore he tauhohenga whakawhiti me ētahi atu raupapatanga tino ōrite o te ira tangata (ira CYP2C19 tangata, ira RPN2 tangata); te whakarerekētanga o te CYP2C9*13 me te VKORC1 (3730G>A) i waho o te awhe kitea o tēnei kete. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Tere-Wā-Tūturu o Applied Biosystems 7500 Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu SLAN-96P Pūnaha PCR Wā-Tūturu LightCycler®480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Te matū tango e taunakitia ana: Kete Macro & Micro-Test Viral DNA/RNA (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
